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Article: An examination of the immune system of the duck (Anas platyrhynchos) for factors resembling some defined mammalian cytokines

TitleAn examination of the immune system of the duck (Anas platyrhynchos) for factors resembling some defined mammalian cytokines
Authors
Issue Date1993
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/devcompimm
Citation
Developmental And Comparative Immunology, 1993, v. 17 n. 4, p. 341-355 How to Cite?
AbstractDuck lymphoblasts generated by phytohaemagglutinin (PHA) did not respond to recombinant or Jurkat cell line human interleukin (IL)-2 or possess surface antigens resembling mammalian IL-2 receptors or IL-1β. Supernatant fluids from normal and PHA-stimulated duck lymphocyte cultures, and normal and lipopolysaccharide (LPS)-stimulated monocytes, gave negative results in a range of assays for biological activity and immunochemical presence of factors resembling mammalian IL-1 and IL-2. However, supernatant fluids from LPS-stimulated duck monocytes contained IL-6-like activity (up to 35 units/mL) assessed on the 7TD-1 murine cell line. We were unable to demonstrate mRNA that would hybridize to cDNA probes for human IL-1β, IL-6, and tumour necrosis factor (TNF) in extracts of blood and lymphoid organs from normal and antigen-stimulated ducks. Because homologous serum or plasma is essential for duck lymphocytes and macrophages to respond to mitogens in vitro, we asked whether this growth-factor-like activity might be caused by substances resembling mammalian cytokines. Serum and plasma were examined for activity consistent with IL-1 and IL-6 on mammalian target cells. None was detected. Instead, both serum and plasma contained inhibitors of human IL-1β and IL-6, detected at dilutions up to 1:100. Inhibition by serum was heat (56°C, 30 min) labile but inhibition by plasma was heat stable. The identities and biological functions of these inhibitors remain to be defined.
Persistent Identifierhttp://hdl.handle.net/10722/147962
ISSN
2015 Impact Factor: 3.62
2015 SCImago Journal Rankings: 1.335
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHiggins, DAen_US
dc.contributor.authorCromie, RLen_US
dc.contributor.authorSrivastava, Gen_US
dc.contributor.authorHerzbeck, Hen_US
dc.contributor.authorSchluter, Cen_US
dc.contributor.authorGerdes, Jen_US
dc.contributor.authorDiamantstein, Ten_US
dc.contributor.authorFlad, HDen_US
dc.date.accessioned2012-05-29T06:10:07Z-
dc.date.available2012-05-29T06:10:07Z-
dc.date.issued1993en_US
dc.identifier.citationDevelopmental And Comparative Immunology, 1993, v. 17 n. 4, p. 341-355en_US
dc.identifier.issn0145-305Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/147962-
dc.description.abstractDuck lymphoblasts generated by phytohaemagglutinin (PHA) did not respond to recombinant or Jurkat cell line human interleukin (IL)-2 or possess surface antigens resembling mammalian IL-2 receptors or IL-1β. Supernatant fluids from normal and PHA-stimulated duck lymphocyte cultures, and normal and lipopolysaccharide (LPS)-stimulated monocytes, gave negative results in a range of assays for biological activity and immunochemical presence of factors resembling mammalian IL-1 and IL-2. However, supernatant fluids from LPS-stimulated duck monocytes contained IL-6-like activity (up to 35 units/mL) assessed on the 7TD-1 murine cell line. We were unable to demonstrate mRNA that would hybridize to cDNA probes for human IL-1β, IL-6, and tumour necrosis factor (TNF) in extracts of blood and lymphoid organs from normal and antigen-stimulated ducks. Because homologous serum or plasma is essential for duck lymphocytes and macrophages to respond to mitogens in vitro, we asked whether this growth-factor-like activity might be caused by substances resembling mammalian cytokines. Serum and plasma were examined for activity consistent with IL-1 and IL-6 on mammalian target cells. None was detected. Instead, both serum and plasma contained inhibitors of human IL-1β and IL-6, detected at dilutions up to 1:100. Inhibition by serum was heat (56°C, 30 min) labile but inhibition by plasma was heat stable. The identities and biological functions of these inhibitors remain to be defined.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/devcompimmen_US
dc.relation.ispartofDevelopmental and Comparative Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCytokines - Analysis - Physiologyen_US
dc.subject.meshDucks - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInterleukin-2 - Pharmacologyen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshMiceen_US
dc.subject.meshRna - Analysisen_US
dc.titleAn examination of the immune system of the duck (Anas platyrhynchos) for factors resembling some defined mammalian cytokinesen_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0145-305X(93)90006-Cen_US
dc.identifier.pmid7690719-
dc.identifier.scopuseid_2-s2.0-0027312582en_US
dc.identifier.volume17en_US
dc.identifier.issue4en_US
dc.identifier.spage341en_US
dc.identifier.epage355en_US
dc.identifier.isiWOS:A1993LP25500006-
dc.publisher.placeUnited Kingdomen_US

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