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Article: Dual-color flow cytometric analysis of megakaryocytic DNA ploidy in the investigation of blastic phase chronic myelogenous leukemia with rearrangement of 3q26

TitleDual-color flow cytometric analysis of megakaryocytic DNA ploidy in the investigation of blastic phase chronic myelogenous leukemia with rearrangement of 3q26
Authors
Keywordschronic myelogenous leukemia
megakaryoblastic transformation
megakaryocytic ploidy
myeloblastic transformation
rearrangement of 3q26
Issue Date1993
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 1993, v. 71 n. 12, p. 3882-3887 How to Cite?
AbstractA patient is described with chronic myelogenous leukemia in blastic crisis, in whom numerous circulating platelet fragments and megakaryocytic nuclei were present, with 50% blasts and 50% micromegakaryocytes in the marrow. The blasts expressed myeloid-associated antigens CD34, CD33, and CD13, whereas the micromegakaryocytes were positive for CD41, CD42b, and CD61. These findings suggested a myeloblastic transformation with a possible megakaryoblastic component. Cytogenetic analysis showed rearrangement of 3q26 in the form of t(2;3) (p13;q26), in addition to t(9;22) (q34;q11). Dual- color flow cytometric analysis of DNA content of CD42b-positive cells showed that the micromegakaryocytes were predominantly 2N, indicating a maturation block before nuclear endoreplication and polyploidization. These findings confirmed a combined myeloblastic and megakaryoblastic transformation. It is concluded that dual-color flow cytometric DNA analysis is a useful method for the investigation of abnormal megakaryocytopoiesis in hematologic malignancies.
Persistent Identifierhttp://hdl.handle.net/10722/147948
ISSN
2015 Impact Factor: 5.649
2015 SCImago Journal Rankings: 3.188
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorRobertson, EPen_HK
dc.contributor.authorLee, CPen_HK
dc.contributor.authorChan, LCen_HK
dc.date.accessioned2012-05-29T06:10:03Z-
dc.date.available2012-05-29T06:10:03Z-
dc.date.issued1993en_HK
dc.identifier.citationCancer, 1993, v. 71 n. 12, p. 3882-3887en_HK
dc.identifier.issn0008-543Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/147948-
dc.description.abstractA patient is described with chronic myelogenous leukemia in blastic crisis, in whom numerous circulating platelet fragments and megakaryocytic nuclei were present, with 50% blasts and 50% micromegakaryocytes in the marrow. The blasts expressed myeloid-associated antigens CD34, CD33, and CD13, whereas the micromegakaryocytes were positive for CD41, CD42b, and CD61. These findings suggested a myeloblastic transformation with a possible megakaryoblastic component. Cytogenetic analysis showed rearrangement of 3q26 in the form of t(2;3) (p13;q26), in addition to t(9;22) (q34;q11). Dual- color flow cytometric analysis of DNA content of CD42b-positive cells showed that the micromegakaryocytes were predominantly 2N, indicating a maturation block before nuclear endoreplication and polyploidization. These findings confirmed a combined myeloblastic and megakaryoblastic transformation. It is concluded that dual-color flow cytometric DNA analysis is a useful method for the investigation of abnormal megakaryocytopoiesis in hematologic malignancies.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_HK
dc.relation.ispartofCanceren_HK
dc.subjectchronic myelogenous leukemiaen_HK
dc.subjectmegakaryoblastic transformationen_HK
dc.subjectmegakaryocytic ploidyen_HK
dc.subjectmyeloblastic transformationen_HK
dc.subjectrearrangement of 3q26en_HK
dc.subject.meshAdulten_US
dc.subject.meshBone Marrow - Pathologyen_US
dc.subject.meshCell Nucleolus - Ultrastructureen_US
dc.subject.meshCell Nucleus - Ultrastructureen_US
dc.subject.meshChromosomes, Human, Pair 3en_US
dc.subject.meshCytoplasm - Ultrastructureen_US
dc.subject.meshDna, Neoplasm - Geneticsen_US
dc.subject.meshDiploidyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGene Rearrangementen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunophenotypingen_US
dc.subject.meshLeukemia, Myeloid, Chronic-Phase - Genetics - Pathologyen_US
dc.subject.meshMegakaryocytes - Metabolism - Pathologyen_US
dc.subject.meshPloidiesen_US
dc.subject.meshPolyploidyen_US
dc.titleDual-color flow cytometric analysis of megakaryocytic DNA ploidy in the investigation of blastic phase chronic myelogenous leukemia with rearrangement of 3q26en_HK
dc.typeArticleen_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8508354-
dc.identifier.scopuseid_2-s2.0-0027160559en_HK
dc.identifier.volume71en_HK
dc.identifier.issue12en_HK
dc.identifier.spage3882en_HK
dc.identifier.epage3887en_HK
dc.identifier.isiWOS:A1993LH25600014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridRobertson, EP=7202391529en_HK
dc.identifier.scopusauthoridLee, CP=7410149538en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK

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