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Article: Amelioration of gentamicin nephrotoxicity by phospholipids

TitleAmelioration of gentamicin nephrotoxicity by phospholipids
Authors
Issue Date1991
PublisherOxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/
Citation
Nephrology Dialysis Transplantation, 1991, v. 6 n. 9, p. 608-614 How to Cite?
AbstractThe effect of phospholipids on gentamicin-induced nephrotoxicity was studied in Sprague-Dawley rats. Group 1a (5 rats) were given daily intraperitoneal injections of 100 mg/kg of gentamicin and sacrificed on day 7. Group 1b (10 rats) were similarly treated but were sacrificed on day 14. Group 2a (5 rats) were given 30 mg/kg of phospholipids for 6 days and sacrificed on day 7, serving as phospholipid controls. Group 2b (5 rats) were similarly treated, and from day 7 onwards daily intraperitoneal injections of 100 mg/kg of gentamicin were given while oral phospholipids were continued until the rats were sacrificed 7 days after gentamicin treatment. Group 2c (10 rats) were treated in the same manner as group 2b but the animals were sacrificed on day 28 after gentamicin treatment. Group 3 (10 rats) were given 30 mg/kg of phospholipids concurrently with intraperitoneal gentamicin injections and were sacrificed on day 28. Protein concentrations, N-acetyl-β-glucosaminidase (NAG) activities and creatinine were measured in 24-h urine samples. Serum creatinine concentrations were measured in blood samples and 24-h creatinine clearance calculated. Gentamicin concentrations were determined in kidney tissues from which sections were also taken for light- and electron-microscopy. Results showed that gentamicin induced a marked increase in NAG and protein excretion, and a marked decrease in creatinine clearance with six rats succumbing to uraemia. Phospholipid treatment, whether started before or concurrently with gentamicin injections, reduced gentamicin-induced nephrotoxicity. The rats did not lose weight. Urinary excretion of NAG and protein was significantly reduced. Serum creatinine concentrations were not increased to the same extent and there were less myelin figures in cytosegregosomes ion electron-microscopy. Gentamicin concentrations in kidney tissues, however, were not altered by the administration of phospholipids.
Persistent Identifierhttp://hdl.handle.net/10722/147882
ISSN
2015 Impact Factor: 4.085
2015 SCImago Journal Rankings: 1.780
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, MKen_US
dc.contributor.authorChan, KWen_US
dc.contributor.authorNg, WLen_US
dc.date.accessioned2012-05-29T06:09:43Z-
dc.date.available2012-05-29T06:09:43Z-
dc.date.issued1991en_US
dc.identifier.citationNephrology Dialysis Transplantation, 1991, v. 6 n. 9, p. 608-614en_US
dc.identifier.issn0931-0509en_US
dc.identifier.urihttp://hdl.handle.net/10722/147882-
dc.description.abstractThe effect of phospholipids on gentamicin-induced nephrotoxicity was studied in Sprague-Dawley rats. Group 1a (5 rats) were given daily intraperitoneal injections of 100 mg/kg of gentamicin and sacrificed on day 7. Group 1b (10 rats) were similarly treated but were sacrificed on day 14. Group 2a (5 rats) were given 30 mg/kg of phospholipids for 6 days and sacrificed on day 7, serving as phospholipid controls. Group 2b (5 rats) were similarly treated, and from day 7 onwards daily intraperitoneal injections of 100 mg/kg of gentamicin were given while oral phospholipids were continued until the rats were sacrificed 7 days after gentamicin treatment. Group 2c (10 rats) were treated in the same manner as group 2b but the animals were sacrificed on day 28 after gentamicin treatment. Group 3 (10 rats) were given 30 mg/kg of phospholipids concurrently with intraperitoneal gentamicin injections and were sacrificed on day 28. Protein concentrations, N-acetyl-β-glucosaminidase (NAG) activities and creatinine were measured in 24-h urine samples. Serum creatinine concentrations were measured in blood samples and 24-h creatinine clearance calculated. Gentamicin concentrations were determined in kidney tissues from which sections were also taken for light- and electron-microscopy. Results showed that gentamicin induced a marked increase in NAG and protein excretion, and a marked decrease in creatinine clearance with six rats succumbing to uraemia. Phospholipid treatment, whether started before or concurrently with gentamicin injections, reduced gentamicin-induced nephrotoxicity. The rats did not lose weight. Urinary excretion of NAG and protein was significantly reduced. Serum creatinine concentrations were not increased to the same extent and there were less myelin figures in cytosegregosomes ion electron-microscopy. Gentamicin concentrations in kidney tissues, however, were not altered by the administration of phospholipids.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://ndt.oxfordjournals.org/en_US
dc.relation.ispartofNephrology Dialysis Transplantationen_US
dc.subject.meshAcetylglucosaminidase - Urineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCreatinine - Blooden_US
dc.subject.meshGentamicins - Antagonists & Inhibitors - Pharmacokinetics - Toxicityen_US
dc.subject.meshKidney - Drug Effects - Metabolism - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshPhospholipids - Pharmacologyen_US
dc.subject.meshProteinuria - Chemically Induceden_US
dc.subject.meshRatsen_US
dc.titleAmelioration of gentamicin nephrotoxicity by phospholipidsen_US
dc.typeArticleen_US
dc.identifier.emailChan, KW:hrmtckw@hku.hken_US
dc.identifier.authorityChan, KW=rp00330en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1745384-
dc.identifier.scopuseid_2-s2.0-0025807758en_US
dc.identifier.volume6en_US
dc.identifier.issue9en_US
dc.identifier.spage608en_US
dc.identifier.epage614en_US
dc.identifier.isiWOS:A1991GM02800002-
dc.publisher.placeUnited Kingdomen_US

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