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Article: The role of alternative splicing patterns of BCR/ABL transcripts in the generation of the blast crisis of chronic myeloid leukaemia

TitleThe role of alternative splicing patterns of BCR/ABL transcripts in the generation of the blast crisis of chronic myeloid leukaemia
Authors
Issue Date1990
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH
Citation
British Journal Of Haematology, 1990, v. 76 n. 1, p. 33-38 How to Cite?
AbstractThree major types of mRNA can be expressed as a result of the Philadelphia translocation, dependent on the position of the break within the BCR gene on chromosome 22. In addition, alternative splicing of the mRNA transcribed from the BCR/ABL fusion gene has been reported and it has been suggested that this may play a role in the generation of the acute phase of Philadelphia positive chronic myeloid leukaemia (CML). We have examined the fusion RNA present in 24 cases of chronic phase CML and 21 cases of patients with CML in blast crisis using the polymerase chain reaction. In no case was it possible to detect the presence of the e1a2 junction which encodes the p190 hybrid protein product. We conclude that the acquisition of the p190 does not play a significant role in the generation of the blast crisis of CML. Neither could we detect a significant difference in the number of cases which simultaneously express both b2a2 and b3a2 junction products in samples isolated from chronic phase and blast crisis. In the series analysed by ethidium bromide stained gels, there was, however, an increase in the percent-age of cases expressing the b3a2 junction in the mononuclear cells of blast crisis patients as compared to the white blood cells of patients in chronic phase.
Persistent Identifierhttp://hdl.handle.net/10722/147859
ISSN
2015 Impact Factor: 5.401
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMorgan, GJen_US
dc.contributor.authorHernandez, Aen_US
dc.contributor.authorChan, LCen_US
dc.contributor.authorHughes, Ten_US
dc.contributor.authorMartiat, Pen_US
dc.contributor.authorWiedemann, LMen_US
dc.date.accessioned2012-05-29T06:09:36Z-
dc.date.available2012-05-29T06:09:36Z-
dc.date.issued1990en_US
dc.identifier.citationBritish Journal Of Haematology, 1990, v. 76 n. 1, p. 33-38en_US
dc.identifier.issn0007-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/147859-
dc.description.abstractThree major types of mRNA can be expressed as a result of the Philadelphia translocation, dependent on the position of the break within the BCR gene on chromosome 22. In addition, alternative splicing of the mRNA transcribed from the BCR/ABL fusion gene has been reported and it has been suggested that this may play a role in the generation of the acute phase of Philadelphia positive chronic myeloid leukaemia (CML). We have examined the fusion RNA present in 24 cases of chronic phase CML and 21 cases of patients with CML in blast crisis using the polymerase chain reaction. In no case was it possible to detect the presence of the e1a2 junction which encodes the p190 hybrid protein product. We conclude that the acquisition of the p190 does not play a significant role in the generation of the blast crisis of CML. Neither could we detect a significant difference in the number of cases which simultaneously express both b2a2 and b3a2 junction products in samples isolated from chronic phase and blast crisis. In the series analysed by ethidium bromide stained gels, there was, however, an increase in the percent-age of cases expressing the b3a2 junction in the mononuclear cells of blast crisis patients as compared to the white blood cells of patients in chronic phase.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJHen_US
dc.relation.ispartofBritish Journal of Haematologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlast Crisis - Geneticsen_US
dc.subject.meshElectrophoresis, Agar Gelen_US
dc.subject.meshFusion Proteins, Bcr-Abl - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukemia, Myelogenous, Chronic, Bcr-Abl Positive - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna Splicing - Physiologyen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshTranscription, Genetic - Physiologyen_US
dc.titleThe role of alternative splicing patterns of BCR/ABL transcripts in the generation of the blast crisis of chronic myeloid leukaemiaen_US
dc.typeArticleen_US
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_US
dc.identifier.authorityChan, LC=rp00373en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2223646-
dc.identifier.scopuseid_2-s2.0-0025153520en_US
dc.identifier.volume76en_US
dc.identifier.issue1en_US
dc.identifier.spage33en_US
dc.identifier.epage38en_US
dc.identifier.isiWOS:A1990DY68300006-
dc.publisher.placeUnited Kingdomen_US

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