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Article: Drug induction of P450IIB1/IIB2 and 5-aminolevulinate synthase mRNAs in rat tissues

TitleDrug induction of P450IIB1/IIB2 and 5-aminolevulinate synthase mRNAs in rat tissues
Authors
Issue Date1989
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
Citation
Biochimica Et Biophysica Acta - Gene Structure And Expression, 1989, v. 1007 n. 2, p. 192-195 How to Cite?
AbstractExpression of the phenobarbitone-inducible cytochrome P-450 mRNA species (cytochrome P450IIB1/IIB2) has been investigated in tissues of rats following administration of 2-allyl-2-isopropylacetamide or phenobarbitone. Using a cDNA probe complementary to these mRNAs, a 2.1 kb mRNA species was detected in liver and lung of untreated rats and to a lesser extent in kidney. This species was not detected in testis, brain or erythroid spleen. Following drug treatment, this mRNA species was increased in liver and kidney, particularly after administration of 2-allyl-2-isopropylacetamide. Using oligomeric probes specific for cytochrome P-450 IIB1/IIB2 mRNAs it was shown that both mRNAs are present in untreated liver and are induced by drug treatment, while in lung only cytochrome P-450 IIB1 was detected and this was not induced. However, in kidney, neither of these mRNAs could be detected, even after drug treatment. This indicates that either the levels of these mRNAs in kidney are below the limit of detection or that a related mRNA is induced which does not hybridize to the oligomeric probes. This represents the first report of a phenobarbitone-inducible cytochrome P-450 mRNA in rat kidney. The tissue-specific drug induction of cytochrome P-450 mRNAs correlated with an increased level of mRNA for 5-aminolevulinate synthase. This finding is compatible with the proposal that induction of 5-aminolevulinate synthase is dependent upon the lowering of heme levels through increased amounts of cytochrome P-450 apoprotein.
Persistent Identifierhttp://hdl.handle.net/10722/147842
ISSN
2007 Impact Factor: 1.704
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSrivastava, Gen_US
dc.contributor.authorBawden, MJen_US
dc.contributor.authorAnderson, Aen_US
dc.contributor.authorMay, BKen_US
dc.date.accessioned2012-05-29T06:09:30Z-
dc.date.available2012-05-29T06:09:30Z-
dc.date.issued1989en_US
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 1989, v. 1007 n. 2, p. 192-195en_US
dc.identifier.issn0167-4781en_US
dc.identifier.urihttp://hdl.handle.net/10722/147842-
dc.description.abstractExpression of the phenobarbitone-inducible cytochrome P-450 mRNA species (cytochrome P450IIB1/IIB2) has been investigated in tissues of rats following administration of 2-allyl-2-isopropylacetamide or phenobarbitone. Using a cDNA probe complementary to these mRNAs, a 2.1 kb mRNA species was detected in liver and lung of untreated rats and to a lesser extent in kidney. This species was not detected in testis, brain or erythroid spleen. Following drug treatment, this mRNA species was increased in liver and kidney, particularly after administration of 2-allyl-2-isopropylacetamide. Using oligomeric probes specific for cytochrome P-450 IIB1/IIB2 mRNAs it was shown that both mRNAs are present in untreated liver and are induced by drug treatment, while in lung only cytochrome P-450 IIB1 was detected and this was not induced. However, in kidney, neither of these mRNAs could be detected, even after drug treatment. This indicates that either the levels of these mRNAs in kidney are below the limit of detection or that a related mRNA is induced which does not hybridize to the oligomeric probes. This represents the first report of a phenobarbitone-inducible cytochrome P-450 mRNA in rat kidney. The tissue-specific drug induction of cytochrome P-450 mRNAs correlated with an increased level of mRNA for 5-aminolevulinate synthase. This finding is compatible with the proposal that induction of 5-aminolevulinate synthase is dependent upon the lowering of heme levels through increased amounts of cytochrome P-450 apoprotein.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexpen_US
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expressionen_US
dc.subject.mesh5-Aminolevulinate Synthetase - Biosynthesisen_US
dc.subject.meshAllylisopropylacetamide - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCytochrome P-450 Enzyme System - Biosynthesisen_US
dc.subject.meshEnzyme Induction - Drug Effectsen_US
dc.subject.meshKidney - Enzymologyen_US
dc.subject.meshLiver - Enzymologyen_US
dc.subject.meshLung - Enzymologyen_US
dc.subject.meshMaleen_US
dc.subject.meshPhenobarbital - Pharmacologyen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshTissue Distributionen_US
dc.titleDrug induction of P450IIB1/IIB2 and 5-aminolevulinate synthase mRNAs in rat tissuesen_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2920173-
dc.identifier.scopuseid_2-s2.0-0024586425en_US
dc.identifier.volume1007en_US
dc.identifier.issue2en_US
dc.identifier.spage192en_US
dc.identifier.epage195en_US
dc.identifier.isiWOS:A1989T653600008-
dc.publisher.placeNetherlandsen_US

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