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Article: Regulation of 5-aminolevulinate synthase mRNA in different rat tissues

TitleRegulation of 5-aminolevulinate synthase mRNA in different rat tissues
Authors
Issue Date1988
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1988, v. 263 n. 11, p. 5202-5209 How to Cite?
AbstractcDNA clones for rat liver 5-aminolevulinate synthase have been isolated and used to examine mRNA levels in different rat tissues. Northern hybridization analysis of total RNA from various rat tissues showed the presence of a single 5-aminolevulinate synthase mRNA species of estimated length 2.3 kilobases. Primer extension and RNase mapping studies indicated that the mRNA is identical in all tissues. Highest basal levels were seen in liver and heart. Administration of hemin to rats reduced the basal level of this mRNA only in liver but the heme precursor, 5-aminolevulinate (or its methyl ester), repressed the basal levels in liver, kidney, heart, testis, and brain. The drug 2-allyl-2-isopropylacetamide increased the mRNA level in liver and kidney only while human chorionic gonadotropin hormone elevated the level in testis. Administration of the heme precursor 5-aminolevulinate prevented these inductions. Nuclear transcriptional run-off experiments in liver cell nuclei showed that 2-allyl-2-isopropylacetamide and 5-aminolevulinate exert their effect by altering the rate of transcription of the 5-aminolevulinate synthase gene. The results indicate that a single 5-aminolevulinate synthase mRNA is expressed in all tissues and that its transcription is negatively regulated by heme.
Persistent Identifierhttp://hdl.handle.net/10722/147818
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSrivastava, Gen_US
dc.contributor.authorBorthwick, IAen_US
dc.contributor.authorMaguire, DJen_US
dc.contributor.authorElferink, CJen_US
dc.contributor.authorBawden, MJen_US
dc.contributor.authorMercer, JFBen_US
dc.contributor.authorMay, BKen_US
dc.date.accessioned2012-05-29T06:09:22Z-
dc.date.available2012-05-29T06:09:22Z-
dc.date.issued1988en_US
dc.identifier.citationJournal Of Biological Chemistry, 1988, v. 263 n. 11, p. 5202-5209en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/147818-
dc.description.abstractcDNA clones for rat liver 5-aminolevulinate synthase have been isolated and used to examine mRNA levels in different rat tissues. Northern hybridization analysis of total RNA from various rat tissues showed the presence of a single 5-aminolevulinate synthase mRNA species of estimated length 2.3 kilobases. Primer extension and RNase mapping studies indicated that the mRNA is identical in all tissues. Highest basal levels were seen in liver and heart. Administration of hemin to rats reduced the basal level of this mRNA only in liver but the heme precursor, 5-aminolevulinate (or its methyl ester), repressed the basal levels in liver, kidney, heart, testis, and brain. The drug 2-allyl-2-isopropylacetamide increased the mRNA level in liver and kidney only while human chorionic gonadotropin hormone elevated the level in testis. Administration of the heme precursor 5-aminolevulinate prevented these inductions. Nuclear transcriptional run-off experiments in liver cell nuclei showed that 2-allyl-2-isopropylacetamide and 5-aminolevulinate exert their effect by altering the rate of transcription of the 5-aminolevulinate synthase gene. The results indicate that a single 5-aminolevulinate synthase mRNA is expressed in all tissues and that its transcription is negatively regulated by heme.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.mesh5-Aminolevulinate Synthetase - Geneticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCodonen_US
dc.subject.meshDna - Analysisen_US
dc.subject.meshHemin - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshRibonucleases - Metabolismen_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleRegulation of 5-aminolevulinate synthase mRNA in different rat tissuesen_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3356687-
dc.identifier.scopuseid_2-s2.0-0023923103en_US
dc.identifier.volume263en_US
dc.identifier.issue11en_US
dc.identifier.spage5202en_US
dc.identifier.epage5209en_US
dc.identifier.isiWOS:A1988M886000038-
dc.publisher.placeUnited Statesen_US

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