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Article: A unique gene for 5-aminolevulinate synthase in chickens. Evidence for expression of an identical messenger RNA in hepatic and erythroid tissues.

TitleA unique gene for 5-aminolevulinate synthase in chickens. Evidence for expression of an identical messenger RNA in hepatic and erythroid tissues.
Authors
Issue Date1987
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1987, v. 262 n. 9, p. 3988-3992 How to Cite?
AbstractReports to date have led to the conclusion that there are isozymes for 5-aminolevulinate synthase in the liver and erythroid tissue of chicken. Indeed, the existence of a multigene family for chicken 5-aminolevulinate synthase has been proposed. We find no evidence to support these proposals. In this work we show that 5-aminolevulinate synthase mRNA from chicken liver and reticulocytes is identical as determined by RNase mapping and primer extension studies and that the 5-aminolevulinate synthase protein from these tissues is the same size as judged by immunoblot analysis. We also show that a single mRNA species for 5-aminolevulinate synthase is present in chicken liver, reticulocytes, brain, and heart and an avian erythroblastosis virus-transformed chicken erythroblast cell line. Southern analysis shows the presence of only one gene copy for 5-aminolevulinate synthase in the chicken haploid genome. Overall, these results lead to the conclusion that in chickens 5-aminolevulinate synthase is encoded by a unique gene and is expressed as a single mRNA species in all tissues.
Persistent Identifierhttp://hdl.handle.net/10722/147806
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorElferink, CJen_US
dc.contributor.authorSrivastava, Gen_US
dc.contributor.authorMaguire, DJen_US
dc.contributor.authorBorthwick, IAen_US
dc.contributor.authorMay, BKen_US
dc.contributor.authorElliott, WHen_US
dc.date.accessioned2012-05-29T06:09:19Z-
dc.date.available2012-05-29T06:09:19Z-
dc.date.issued1987en_US
dc.identifier.citationJournal Of Biological Chemistry, 1987, v. 262 n. 9, p. 3988-3992en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/147806-
dc.description.abstractReports to date have led to the conclusion that there are isozymes for 5-aminolevulinate synthase in the liver and erythroid tissue of chicken. Indeed, the existence of a multigene family for chicken 5-aminolevulinate synthase has been proposed. We find no evidence to support these proposals. In this work we show that 5-aminolevulinate synthase mRNA from chicken liver and reticulocytes is identical as determined by RNase mapping and primer extension studies and that the 5-aminolevulinate synthase protein from these tissues is the same size as judged by immunoblot analysis. We also show that a single mRNA species for 5-aminolevulinate synthase is present in chicken liver, reticulocytes, brain, and heart and an avian erythroblastosis virus-transformed chicken erythroblast cell line. Southern analysis shows the presence of only one gene copy for 5-aminolevulinate synthase in the chicken haploid genome. Overall, these results lead to the conclusion that in chickens 5-aminolevulinate synthase is encoded by a unique gene and is expressed as a single mRNA species in all tissues.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.mesh5-Aminolevulinate Synthetase - Geneticsen_US
dc.subject.meshAlpharetrovirusen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain Chemistryen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Transformation, Viralen_US
dc.subject.meshChickensen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshDna, Recombinanten_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshErythroblasts - Analysisen_US
dc.subject.meshImmunologic Testsen_US
dc.subject.meshLiver - Analysisen_US
dc.subject.meshMyocardium - Analysisen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshReticulocytes - Analysisen_US
dc.subject.meshRibonucleasesen_US
dc.titleA unique gene for 5-aminolevulinate synthase in chickens. Evidence for expression of an identical messenger RNA in hepatic and erythroid tissues.en_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3558402-
dc.identifier.scopuseid_2-s2.0-0023664110en_US
dc.identifier.volume262en_US
dc.identifier.issue9en_US
dc.identifier.spage3988en_US
dc.identifier.epage3992en_US
dc.identifier.isiWOS:A1987G578600013-
dc.publisher.placeUnited Statesen_US

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