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Article: Modified alkali digestion method for pulmonary asbestos fibre counts

TitleModified alkali digestion method for pulmonary asbestos fibre counts
Authors
Issue Date1987
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00313025.asp
Citation
Pathology, 1987, v. 19 n. 2, p. 159-161 How to Cite?
AbstractIn using the standard alkali digestion method for pulmonary asbestos fibre count, it was found that carbonaceous particles often obscured the presence of asbestos bodies (coated fibres) rendering their quantification inaccurate, particularly in lungs with a high soot particle content and a low fibre count. We applied the chloroform-ethanol separation technique from the bleaching agent digestion method to the alkali digestion method to remove the carbonaceous particles. It was found that coated fibres are much easier to visualize and count. The uncoated fibre count was nevertheless more accurate with the original method. The coated and uncoated fibre counts from 21 lung specimens were compared using both methods. The total fibre count was considered better derived from using both techniques.
Persistent Identifierhttp://hdl.handle.net/10722/147790
ISSN
2015 Impact Factor: 2.968
2015 SCImago Journal Rankings: 1.049
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, PCen_US
dc.contributor.authorChung, WBen_US
dc.contributor.authorChan, KWen_US
dc.contributor.authorKung, ITMen_US
dc.date.accessioned2012-05-29T06:09:14Z-
dc.date.available2012-05-29T06:09:14Z-
dc.date.issued1987en_US
dc.identifier.citationPathology, 1987, v. 19 n. 2, p. 159-161en_US
dc.identifier.issn0031-3025en_US
dc.identifier.urihttp://hdl.handle.net/10722/147790-
dc.description.abstractIn using the standard alkali digestion method for pulmonary asbestos fibre count, it was found that carbonaceous particles often obscured the presence of asbestos bodies (coated fibres) rendering their quantification inaccurate, particularly in lungs with a high soot particle content and a low fibre count. We applied the chloroform-ethanol separation technique from the bleaching agent digestion method to the alkali digestion method to remove the carbonaceous particles. It was found that coated fibres are much easier to visualize and count. The uncoated fibre count was nevertheless more accurate with the original method. The coated and uncoated fibre counts from 21 lung specimens were compared using both methods. The total fibre count was considered better derived from using both techniques.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00313025.aspen_US
dc.relation.ispartofPathologyen_US
dc.subject.meshAlkalies - Diagnostic Use - Pharmacologyen_US
dc.subject.meshAsbestos - Analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshLung - Analysis - Drug Effectsen_US
dc.subject.meshMethodsen_US
dc.titleModified alkali digestion method for pulmonary asbestos fibre countsen_US
dc.typeArticleen_US
dc.identifier.emailChan, KW:hrmtckw@hku.hken_US
dc.identifier.authorityChan, KW=rp00330en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3453997en_US
dc.identifier.scopuseid_2-s2.0-0023189818en_US
dc.identifier.volume19en_US
dc.identifier.issue2en_US
dc.identifier.spage159en_US
dc.identifier.epage161en_US
dc.identifier.isiWOS:A1987H219200009-
dc.publisher.placeUnited Kingdomen_US

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