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Article: Molecular cloning of hepatic 5-aminolevulinate synthase.

TitleMolecular cloning of hepatic 5-aminolevulinate synthase.
Authors
Issue Date1984
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 1984, v. 144 n. 1, p. 95-99 How to Cite?
AbstractHepatic 5-aminolevulinate synthase was induced in chick embryos by administration of the porphyrinogenic drugs 2-allyl-2-isopropylacetamide and 3,5-diethoxycarbonyl-1,4-dihydrocollidine. A cDNA library was constructed from drug-induced liver mRNA and clones containing sequences coding for 5-aminolevulinate synthase were identified by hybrid-selected translation. The identity of these clones was confirmed by comparing DNA sequence data with the amino acid sequence of peptides from purified 5-aminolevulinate synthase. From Northern blot analysis the size of the mRNA for 5-aminolevulinate synthase was estimated to be 2800 base pairs, approximately 600 base pairs more than that required to code for the primary translation product of relative molecular mass 74000.
Persistent Identifierhttp://hdl.handle.net/10722/147747
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBorthwick, IAen_US
dc.contributor.authorSrivastava, Gen_US
dc.contributor.authorHobbs, AAen_US
dc.contributor.authorPirola, BAen_US
dc.contributor.authorBrooker, JDen_US
dc.contributor.authorMay, BKen_US
dc.contributor.authorElliott, WHen_US
dc.date.accessioned2012-05-29T06:09:02Z-
dc.date.available2012-05-29T06:09:02Z-
dc.date.issued1984en_US
dc.identifier.citationEuropean Journal Of Biochemistry, 1984, v. 144 n. 1, p. 95-99en_US
dc.identifier.issn0014-2956en_US
dc.identifier.urihttp://hdl.handle.net/10722/147747-
dc.description.abstractHepatic 5-aminolevulinate synthase was induced in chick embryos by administration of the porphyrinogenic drugs 2-allyl-2-isopropylacetamide and 3,5-diethoxycarbonyl-1,4-dihydrocollidine. A cDNA library was constructed from drug-induced liver mRNA and clones containing sequences coding for 5-aminolevulinate synthase were identified by hybrid-selected translation. The identity of these clones was confirmed by comparing DNA sequence data with the amino acid sequence of peptides from purified 5-aminolevulinate synthase. From Northern blot analysis the size of the mRNA for 5-aminolevulinate synthase was estimated to be 2800 base pairs, approximately 600 base pairs more than that required to code for the primary translation product of relative molecular mass 74000.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_US
dc.relation.ispartofEuropean Journal of Biochemistryen_US
dc.subject.mesh5-Aminolevulinate Synthetase - Biosynthesis - Geneticsen_US
dc.subject.meshAllylisopropylacetamide - Pharmacologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna - Isolation & Purificationen_US
dc.subject.meshDicarbethoxydihydrocollidine - Pharmacologyen_US
dc.subject.meshEnzyme Induction - Drug Effectsen_US
dc.subject.meshLiver - Enzymologyen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPolyribosomes - Metabolismen_US
dc.subject.meshProtein Biosynthesis - Drug Effectsen_US
dc.subject.meshRna, Messenger - Isolation & Purificationen_US
dc.titleMolecular cloning of hepatic 5-aminolevulinate synthase.en_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6548188-
dc.identifier.scopuseid_2-s2.0-0021503581en_US
dc.identifier.volume144en_US
dc.identifier.issue1en_US
dc.identifier.spage95en_US
dc.identifier.epage99en_US
dc.identifier.isiWOS:A1984TN89500013-
dc.publisher.placeUnited Kingdomen_US

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