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Article: Effect of lead ions on chick-embryo liver mitochondrial δ-aminolaevulinate synthase

TitleEffect of lead ions on chick-embryo liver mitochondrial δ-aminolaevulinate synthase
Authors
Issue Date1984
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 1984, v. 222 n. 3, p. 627-630 How to Cite?
AbstractPb2+ activated native chick-embryo liver mitochondrial δ-aminolaevulinate synthase (EC 2.3.1.37). This result contradicted with the inhibitory effect observed by earlier workers who used degraded enzyme preparations. Enzyme activation was biphasic. An initial activation phase was observed with Pb2+ concentrations up to 200 μM, and a secondary phase with concentrations from 200 μM to at least 2 mM. Maximum primary activation was 2.5-fold at 200 μM-Pb2+, with a further 2-fold activation observed at 2 mM-Pb2+. Primary activation was not affected by a 10-fold molar excess of dithioerythritol, but the secondary activation was abolished by dithioerythritol. Secondary-phase activation was lost upon increasing time of incubation of the enzyme with Pb2+. The implications of these findings are discussed with reference to lead poisoning and the mechanism of δ-aminolaevulinate synthase.
Persistent Identifierhttp://hdl.handle.net/10722/147740
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPirola, BAen_US
dc.contributor.authorBorthwick, IAen_US
dc.contributor.authorSrivastava, Gen_US
dc.date.accessioned2012-05-29T06:09:00Z-
dc.date.available2012-05-29T06:09:00Z-
dc.date.issued1984en_US
dc.identifier.citationBiochemical Journal, 1984, v. 222 n. 3, p. 627-630en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/147740-
dc.description.abstractPb2+ activated native chick-embryo liver mitochondrial δ-aminolaevulinate synthase (EC 2.3.1.37). This result contradicted with the inhibitory effect observed by earlier workers who used degraded enzyme preparations. Enzyme activation was biphasic. An initial activation phase was observed with Pb2+ concentrations up to 200 μM, and a secondary phase with concentrations from 200 μM to at least 2 mM. Maximum primary activation was 2.5-fold at 200 μM-Pb2+, with a further 2-fold activation observed at 2 mM-Pb2+. Primary activation was not affected by a 10-fold molar excess of dithioerythritol, but the secondary activation was abolished by dithioerythritol. Secondary-phase activation was lost upon increasing time of incubation of the enzyme with Pb2+. The implications of these findings are discussed with reference to lead poisoning and the mechanism of δ-aminolaevulinate synthase.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.mesh5-Aminolevulinate Synthetase - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshDithioerythritol - Pharmacologyen_US
dc.subject.meshEnzyme Activation - Drug Effectsen_US
dc.subject.meshLead - Pharmacologyen_US
dc.subject.meshMitochondria, Liver - Enzymologyen_US
dc.subject.meshTime Factorsen_US
dc.titleEffect of lead ions on chick-embryo liver mitochondrial δ-aminolaevulinate synthaseen_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6487266-
dc.identifier.scopuseid_2-s2.0-0021145407en_US
dc.identifier.volume222en_US
dc.identifier.issue3en_US
dc.identifier.spage627en_US
dc.identifier.epage630en_US
dc.identifier.isiWOS:A1984TL25200008-
dc.publisher.placeUnited Kingdomen_US

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