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Article: Evidence that 2-allyl-2-isopropylacetamide, phenobarbital and 3,5-diethoxycarbonyl-1,4-dihydrocollidine induce the same cytochrome P450 mRNA in chick embryo liver

TitleEvidence that 2-allyl-2-isopropylacetamide, phenobarbital and 3,5-diethoxycarbonyl-1,4-dihydrocollidine induce the same cytochrome P450 mRNA in chick embryo liver
Authors
Issue Date1983
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 1983, v. 136 n. 2, p. 327-332 How to Cite?
AbstractThe induction of cytochrome P450 in chick embryo liver has been studied using three different porphyrinogenic drugs, 2-allyl-2-isopropylacetamide, 3,5-diethoxycarbonyl-1,4-dihydrocollidine and phenobarbital. Pulse-labelling studies have shown that for each drug the cytochrome P450 synthesised either in ovo or in a wheat germ translation system reacted immunologically with antibody raised against the purified 2-allyl-2-isopropylacetamide-induced enzyme (M(r) = 50,000). To investigate whether this is due to the three drugs inducing the same protein or different proteins with common immunological determinants, nucleic acid hybridization studies have been carried out using a recently characterised 2-allyl-2-isopropylacetamide-induced cytochrome P450 cloned cDNA probe. It has been shown that the mRNA induced by each drug hybridizes within this probe and all are of similar size. The melting profile of the mRNA · cDNA hybrids indicates that the mRNAs induced by the three drugs have at least 98% homology with the cDNA probe. Restriction endonuclease digestions of total chick embryo genomal DNA and a chick cytochrome P450 genomal clone indicates that the cytochrome P450 gene homologous with the cDNA probe is represented in the genome only once. These results strongly suggest that the three drugs cause increased levels of the same cytochrome P450 mRNA, possibly due to enhanced expression of the same gene. Results are also presented which show that other cytochrome P450-inducing drugs, 3-methylcholanthrene, β-naphthoflavone or pregnenolone-16α-carbonitrile do not increase the level of the 2-allyl-2-isopropylacetamide-inducible mRNA but rather reduce it to a level which was lower than that of the untreated controls.
Persistent Identifierhttp://hdl.handle.net/10722/147739
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBrooker, JDen_US
dc.contributor.authorSrivastava, Gen_US
dc.contributor.authorBorthwick, IAen_US
dc.date.accessioned2012-05-29T06:09:00Z-
dc.date.available2012-05-29T06:09:00Z-
dc.date.issued1983en_US
dc.identifier.citationEuropean Journal Of Biochemistry, 1983, v. 136 n. 2, p. 327-332en_US
dc.identifier.issn0014-2956en_US
dc.identifier.urihttp://hdl.handle.net/10722/147739-
dc.description.abstractThe induction of cytochrome P450 in chick embryo liver has been studied using three different porphyrinogenic drugs, 2-allyl-2-isopropylacetamide, 3,5-diethoxycarbonyl-1,4-dihydrocollidine and phenobarbital. Pulse-labelling studies have shown that for each drug the cytochrome P450 synthesised either in ovo or in a wheat germ translation system reacted immunologically with antibody raised against the purified 2-allyl-2-isopropylacetamide-induced enzyme (M(r) = 50,000). To investigate whether this is due to the three drugs inducing the same protein or different proteins with common immunological determinants, nucleic acid hybridization studies have been carried out using a recently characterised 2-allyl-2-isopropylacetamide-induced cytochrome P450 cloned cDNA probe. It has been shown that the mRNA induced by each drug hybridizes within this probe and all are of similar size. The melting profile of the mRNA · cDNA hybrids indicates that the mRNAs induced by the three drugs have at least 98% homology with the cDNA probe. Restriction endonuclease digestions of total chick embryo genomal DNA and a chick cytochrome P450 genomal clone indicates that the cytochrome P450 gene homologous with the cDNA probe is represented in the genome only once. These results strongly suggest that the three drugs cause increased levels of the same cytochrome P450 mRNA, possibly due to enhanced expression of the same gene. Results are also presented which show that other cytochrome P450-inducing drugs, 3-methylcholanthrene, β-naphthoflavone or pregnenolone-16α-carbonitrile do not increase the level of the 2-allyl-2-isopropylacetamide-inducible mRNA but rather reduce it to a level which was lower than that of the untreated controls.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_US
dc.relation.ispartofEuropean Journal of Biochemistryen_US
dc.subject.meshAcetamides - Pharmacologyen_US
dc.subject.meshAllylisopropylacetamide - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChemical Phenomenaen_US
dc.subject.meshChemistryen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshCytochrome P-450 Enzyme System - Biosynthesisen_US
dc.subject.meshDnaen_US
dc.subject.meshDicarbethoxydihydrocollidine - Pharmacologyen_US
dc.subject.meshEnzyme Induction - Drug Effectsen_US
dc.subject.meshLiver - Enzymologyen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPhenobarbital - Pharmacologyen_US
dc.subject.meshPyridines - Pharmacologyen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.titleEvidence that 2-allyl-2-isopropylacetamide, phenobarbital and 3,5-diethoxycarbonyl-1,4-dihydrocollidine induce the same cytochrome P450 mRNA in chick embryo liveren_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6688781-
dc.identifier.scopuseid_2-s2.0-0021062267en_US
dc.identifier.volume136en_US
dc.identifier.issue2en_US
dc.identifier.spage327en_US
dc.identifier.epage332en_US
dc.identifier.isiWOS:A1983RQ53300014-
dc.publisher.placeUnited Kingdomen_US

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