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Article: Radiochemical assay for δ-aminolevulinate synthase

TitleRadiochemical assay for δ-aminolevulinate synthase
Authors
Issue Date1982
Citation
Enzyme, 1982, v. 28 n. 2-3, p. 109-117 How to Cite?
AbstractWe describe here a standard protocol for the radiochemical assay of δ-aminolevulinate synthase from 17-day chick embryo liver mitochondria and starved male rat liver mitochondria. The assay is sensitive to at least 15 pmol of the δ-aminolevulinate produced and can be adapted to suit most tissue types. A number of possible modifications to the procedure is discussed.
Persistent Identifierhttp://hdl.handle.net/10722/147730
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBrooker, JDen_US
dc.contributor.authorSrivastava, Gen_US
dc.contributor.authorMay, BKen_US
dc.contributor.authorElliott, WHen_US
dc.date.accessioned2012-05-29T06:08:57Z-
dc.date.available2012-05-29T06:08:57Z-
dc.date.issued1982en_US
dc.identifier.citationEnzyme, 1982, v. 28 n. 2-3, p. 109-117en_US
dc.identifier.issn0013-9432en_US
dc.identifier.urihttp://hdl.handle.net/10722/147730-
dc.description.abstractWe describe here a standard protocol for the radiochemical assay of δ-aminolevulinate synthase from 17-day chick embryo liver mitochondria and starved male rat liver mitochondria. The assay is sensitive to at least 15 pmol of the δ-aminolevulinate produced and can be adapted to suit most tissue types. A number of possible modifications to the procedure is discussed.en_US
dc.languageengen_US
dc.relation.ispartofEnzymeen_US
dc.subject.mesh5-Aminolevulinate Synthetase - Analysisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarbon Radioisotopes - Diagnostic Useen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshMaleen_US
dc.subject.meshMitochondria, Liver - Enzymologyen_US
dc.subject.meshProteins - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshSuccinates - Pharmacologyen_US
dc.subject.meshSuccinic Aciden_US
dc.titleRadiochemical assay for δ-aminolevulinate synthaseen_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7140714en_US
dc.identifier.scopuseid_2-s2.0-0020347161en_US
dc.identifier.volume28en_US
dc.identifier.issue2-3en_US
dc.identifier.spage109en_US
dc.identifier.epage117en_US
dc.identifier.isiWOS:A1982PK27800004-

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