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Article: Chondroitin sulfates in the developing rat hindbrain confine commissural projections of vestibular nuclear neurons

TitleChondroitin sulfates in the developing rat hindbrain confine commissural projections of vestibular nuclear neurons
Authors
Issue Date2012
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.neuraldevelopment.com
Citation
Neural Development, 2012, v. 7, article no. 6 How to Cite?
AbstractBackground: Establishing correct neuronal circuitry is crucial to proper function of the vertebrate nervous system. The abundance of chondroitin sulfate (CS) proteoglycans in embryonic neural environments suggests that matrix proteoglycans regulate axonal projections when fiber tracts have not yet formed. Among the early-born neurons, the vestibular nucleus (VN) neurons initiate commissural projections soon after generation at E12.5 and reach the contralateral target by E15.5 in the rat hindbrain. We therefore exploited 24-hour cultures (1 day in vitro (DIV)) of the rat embryos and chondroitinase ABC treatment of the hindbrain matrix to reveal the role of CS moieties in axonal initiation and projection in the early hindbrain.Results: DiI tracing from the VN at E12.5 (+1 DIV) showed contralaterally projecting fibers assuming fascicles that hardly reached the midline in the controls. In the enzyme-treated embryos, the majority of fibers were unfasciculated as they crossed the midline at 90°. At E13.5 (+1 DIV), the commissural projections formed fascicles and crossed the midline in the controls. Enzyme treatment apparently did not affect the pioneer axons that had advanced as thick fascicles normal to the midline and beyond, towards the contralateral VN. Later projections, however, traversed the enzyme-treated matrix as unfasciculated fibers, deviated from the normal course crossing the midline at various angles and extending beyond the contralateral VN. This suggests that CSs also limit the course of the later projections, which otherwise would be attracted to alternative targets.Conclusions: CS moieties in the early hindbrain therefore control the course and fasciculation of axonal projections and the timing of axonal arrival at the target. © 2012 Kwok et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/147659
ISSN
2015 Impact Factor: 3.038
2015 SCImago Journal Rankings: 2.199
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7670/06M
Funding Information:

This work was supported by a GRF research grant from the Hong Kong Research Grants Council to YSC and DKYS (HKU 7670/06M). We thank Alice YY Lui, Kimmy FL Tsang and Simon CM Chan of The University of Hong Kong for their excellent technical support in this work.

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DC FieldValueLanguage
dc.contributor.authorKwok, JCFen_HK
dc.contributor.authorYuen, YLen_HK
dc.contributor.authorLau, WKen_HK
dc.contributor.authorZhang, FXen_HK
dc.contributor.authorFawcett, JWen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2012-05-29T06:05:18Z-
dc.date.available2012-05-29T06:05:18Z-
dc.date.issued2012en_HK
dc.identifier.citationNeural Development, 2012, v. 7, article no. 6en_HK
dc.identifier.issn1749-8104en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147659-
dc.description.abstractBackground: Establishing correct neuronal circuitry is crucial to proper function of the vertebrate nervous system. The abundance of chondroitin sulfate (CS) proteoglycans in embryonic neural environments suggests that matrix proteoglycans regulate axonal projections when fiber tracts have not yet formed. Among the early-born neurons, the vestibular nucleus (VN) neurons initiate commissural projections soon after generation at E12.5 and reach the contralateral target by E15.5 in the rat hindbrain. We therefore exploited 24-hour cultures (1 day in vitro (DIV)) of the rat embryos and chondroitinase ABC treatment of the hindbrain matrix to reveal the role of CS moieties in axonal initiation and projection in the early hindbrain.Results: DiI tracing from the VN at E12.5 (+1 DIV) showed contralaterally projecting fibers assuming fascicles that hardly reached the midline in the controls. In the enzyme-treated embryos, the majority of fibers were unfasciculated as they crossed the midline at 90°. At E13.5 (+1 DIV), the commissural projections formed fascicles and crossed the midline in the controls. Enzyme treatment apparently did not affect the pioneer axons that had advanced as thick fascicles normal to the midline and beyond, towards the contralateral VN. Later projections, however, traversed the enzyme-treated matrix as unfasciculated fibers, deviated from the normal course crossing the midline at various angles and extending beyond the contralateral VN. This suggests that CSs also limit the course of the later projections, which otherwise would be attracted to alternative targets.Conclusions: CS moieties in the early hindbrain therefore control the course and fasciculation of axonal projections and the timing of axonal arrival at the target. © 2012 Kwok et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.neuraldevelopment.com-
dc.relation.ispartofNeural Developmenten_HK
dc.rightsNeural Development. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshChondroitin Sulfates - physiology-
dc.subject.meshEfferent Pathways - cytology - embryology - metabolism-
dc.subject.meshNeurons - cytology - metabolism-
dc.subject.meshRhombencephalon - cytology - embryology - metabolism-
dc.subject.meshVestibular Nuclei - cytology - embryology - metabolism-
dc.titleChondroitin sulfates in the developing rat hindbrain confine commissural projections of vestibular nuclear neuronsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailKwok, JC: h9600218@hkusua.hku.hk-
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1186/1749-8104-7-6en_HK
dc.identifier.pmid22305371-
dc.identifier.pmcidPMC3295737-
dc.identifier.scopuseid_2-s2.0-84862816090en_HK
dc.identifier.hkuros209194-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862816090&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.eissn1749-8104-
dc.identifier.isiWOS:000301581200001-
dc.publisher.placeUnited Kingdom-
dc.relation.projectRoles of chondroitin sulfates in axonal growth and patterning in the developing hindbrain-
dc.identifier.scopusauthoridKwok, JCF=23492624000en_HK
dc.identifier.scopusauthoridYuen, YL=55262517000en_HK
dc.identifier.scopusauthoridLau, WK=55262095100en_HK
dc.identifier.scopusauthoridZhang, FX=7404969043en_HK
dc.identifier.scopusauthoridFawcett, JW=7202551686en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.customcontrol.immutablejt 130418-

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