Article: Sequential establishment of stripe patterns in an expanding cell population

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TitleSequential establishment of stripe patterns in an expanding cell population
AuthorsLiu, C
Fu, X1
Liu, L1
Ren, X1
Chau, CKL1
Li, S1
Xiang, L1 4
Zeng, H1
Chen, G1
Tang, LH2
Lenz, P3
Cui, X1
Huang, W1
Hwa, T1
Huang, JD1
Issue Date2011
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
CitationScience, 2011, v. 334 n. 6053, p. 238-241 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.1209042
AbstractPeriodic stripe patterns are ubiquitous in living organisms, yet the underlying developmental processes are complex and difficult to disentangle. We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises from a recurrent aggregation process at the front of the continuously expanding cell population. The number of stripes formed could be tuned by modulating the basal expression of a single gene. The results establish motility control as a simple route to establishing recurrent structures without requiring an extrinsic pacemaker.
ISSN0036-8075
2011 Impact Factor: 31.201
2011 SCImago Journal Rankings: 5.425
DOIhttp://dx.doi.org/10.1126/science.1209042
ISI Accession Number IDWOS:000295833600050
Funding AgencyGrant Number
Hong Kong University (HKU)
HKU Committee
Research Grants Council (RGC)HKU1/CRF/10
HKU Faculty of Medicine
NSF through the Center for Theoretical Biological PhysicsPHY-0822283
State Hessen
RGC of the Hong Kong Special Administrative Region201606
Funding Information:

We are grateful to members of the University of Hong Kong Team for The International Genetic Engineering Machine Competition (iGEM) 2008 for their contribution to the project. We thank H. Berg, A. Courey, A. Danchin, D. Smith, J. Tailleur, and C. Voigt for valuable comments. This project was supported by a Hong Kong University (HKU) University Development Fund, a Small Project Grant from the HKU Committee on Research and Conference Grants, and a Collaborative Research Fund from the Research Grants Council (RGC) (HKU1/CRF/10) to J.D.H., and a HKU Faculty of Medicine Development Fund to W.H. TH is supported by the NSF through the Center for Theoretical Biological Physics (grant PHY-0822283) and additionally acknowledges an HKU Distinguished Visiting Professorship. P.L. acknowledges support through the LOEWE program of the State Hessen. L.H.T. acknowledges support by the RGC of the Hong Kong Special Administrative Region under grant 201606. E. coli strains and plasmids are available under a material transfer agreement with the University of Hong Kong.

ReferencesReferences in Scopus
GrantsProgramming the Second Generation Tumor-targeting Bacteria
DC Field
Value
dc.contributor.authorLiu, C
dc.contributor.authorFu, X
dc.contributor.authorLiu, L
dc.contributor.authorRen, X
dc.contributor.authorChau, CKL
dc.contributor.authorLi, S
dc.contributor.authorXiang, L
dc.contributor.authorZeng, H
dc.contributor.authorChen, G
dc.contributor.authorTang, LH
dc.contributor.authorLenz, P
dc.contributor.authorCui, X
dc.contributor.authorHuang, W
dc.contributor.authorHwa, T
dc.contributor.authorHuang, JD
dc.date.accessioned2012-05-29T06:05:11Z
dc.date.available2012-05-29T06:05:11Z
dc.date.issued2011
dc.description.abstractPeriodic stripe patterns are ubiquitous in living organisms, yet the underlying developmental processes are complex and difficult to disentangle. We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises from a recurrent aggregation process at the front of the continuously expanding cell population. The number of stripes formed could be tuned by modulating the basal expression of a single gene. The results establish motility control as a simple route to establishing recurrent structures without requiring an extrinsic pacemaker.
dc.description.grantProgramming the Second Generation Tumor-targeting Bacteria
dc.description.grantcode103836
dc.description.naturepublished_or_final_version
dc.identifier.citationScience, 2011, v. 334 n. 6053, p. 238-241 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.1209042
dc.identifier.citeulike9905436
dc.identifier.doihttp://dx.doi.org/10.1126/science.1209042
dc.identifier.epage241
dc.identifier.hkuros203767
dc.identifier.hkuros209359
dc.identifier.isiWOS:000295833600050
Funding AgencyGrant Number
Hong Kong University (HKU)
HKU Committee
Research Grants Council (RGC)HKU1/CRF/10
HKU Faculty of Medicine
NSF through the Center for Theoretical Biological PhysicsPHY-0822283
State Hessen
RGC of the Hong Kong Special Administrative Region201606
Funding Information:

We are grateful to members of the University of Hong Kong Team for The International Genetic Engineering Machine Competition (iGEM) 2008 for their contribution to the project. We thank H. Berg, A. Courey, A. Danchin, D. Smith, J. Tailleur, and C. Voigt for valuable comments. This project was supported by a Hong Kong University (HKU) University Development Fund, a Small Project Grant from the HKU Committee on Research and Conference Grants, and a Collaborative Research Fund from the Research Grants Council (RGC) (HKU1/CRF/10) to J.D.H., and a HKU Faculty of Medicine Development Fund to W.H. TH is supported by the NSF through the Center for Theoretical Biological Physics (grant PHY-0822283) and additionally acknowledges an HKU Distinguished Visiting Professorship. P.L. acknowledges support through the LOEWE program of the State Hessen. L.H.T. acknowledges support by the RGC of the Hong Kong Special Administrative Region under grant 201606. E. coli strains and plasmids are available under a material transfer agreement with the University of Hong Kong.

dc.identifier.issn0036-8075
2011 Impact Factor: 31.201
2011 SCImago Journal Rankings: 5.425
dc.identifier.issue6053
dc.identifier.pmid21998392
dc.identifier.scopuseid_2-s2.0-80054124775
dc.identifier.spage238
dc.identifier.urihttp://hdl.handle.net/10722/147646
dc.identifier.volume334
dc.languageeng
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
dc.publisher.placeUnited States
dc.relation.ispartofScience
dc.relation.referencesReferences in Scopus
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshAcyl-Butyrolactones - Metabolism
dc.subject.meshBacterial Load
dc.subject.meshCell Proliferation
dc.subject.meshCulture Media
dc.subject.meshDiffusion
dc.subject.meshEscherichia Coli K12 - Cytology - Genetics - Growth & Development - Physiology
dc.subject.meshGene Expression Regulation, Bacterial
dc.subject.meshGene Regulatory Networks
dc.subject.meshKinetics
dc.subject.meshModels, Biological
dc.subject.meshMovement
dc.subject.meshQuorum Sensing
dc.subject.meshSynthetic Biology
dc.titleSequential establishment of stripe patterns in an expanding cell population
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Baptist University
  3. Universität Marburg
  4. University of California, San Diego