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Article: Sequential establishment of stripe patterns in an expanding cell population
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TitleSequential establishment of stripe patterns in an expanding cell population
 
AuthorsLiu, C1
Fu, X1
Liu, L1
Ren, X1
Chau, CKL1
Li, S1
Xiang, L1 4
Zeng, H1
Chen, G1
Tang, LH2
Lenz, P3
Cui, X1
Huang, W1 1
Hwa, T1
Huang, JD1
 
Issue Date2011
 
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
 
CitationScience, 2011, v. 334 n. 6053, p. 238-241 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.1209042
 
AbstractPeriodic stripe patterns are ubiquitous in living organisms, yet the underlying developmental processes are complex and difficult to disentangle. We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises from a recurrent aggregation process at the front of the continuously expanding cell population. The number of stripes formed could be tuned by modulating the basal expression of a single gene. The results establish motility control as a simple route to establishing recurrent structures without requiring an extrinsic pacemaker.
 
ISSN0036-8075
2012 Impact Factor: 31.027
2012 SCImago Journal Rankings: 10.618
 
DOIhttp://dx.doi.org/10.1126/science.1209042
 
ISI Accession Number IDWOS:000295833600050
Funding AgencyGrant Number
Hong Kong University (HKU)
HKU Committee
Research Grants Council (RGC)HKU1/CRF/10
HKU Faculty of Medicine
NSF through the Center for Theoretical Biological PhysicsPHY-0822283
State Hessen
RGC of the Hong Kong Special Administrative Region201606
Funding Information:

We are grateful to members of the University of Hong Kong Team for The International Genetic Engineering Machine Competition (iGEM) 2008 for their contribution to the project. We thank H. Berg, A. Courey, A. Danchin, D. Smith, J. Tailleur, and C. Voigt for valuable comments. This project was supported by a Hong Kong University (HKU) University Development Fund, a Small Project Grant from the HKU Committee on Research and Conference Grants, and a Collaborative Research Fund from the Research Grants Council (RGC) (HKU1/CRF/10) to J.D.H., and a HKU Faculty of Medicine Development Fund to W.H. TH is supported by the NSF through the Center for Theoretical Biological Physics (grant PHY-0822283) and additionally acknowledges an HKU Distinguished Visiting Professorship. P.L. acknowledges support through the LOEWE program of the State Hessen. L.H.T. acknowledges support by the RGC of the Hong Kong Special Administrative Region under grant 201606. E. coli strains and plasmids are available under a material transfer agreement with the University of Hong Kong.

 
ReferencesReferences in Scopus
 
GrantsProgramming the Second Generation Tumor-targeting Bacteria
 
DC FieldValue
dc.contributor.authorLiu, C
 
dc.contributor.authorFu, X
 
dc.contributor.authorLiu, L
 
dc.contributor.authorRen, X
 
dc.contributor.authorChau, CKL
 
dc.contributor.authorLi, S
 
dc.contributor.authorXiang, L
 
dc.contributor.authorZeng, H
 
dc.contributor.authorChen, G
 
dc.contributor.authorTang, LH
 
dc.contributor.authorLenz, P
 
dc.contributor.authorCui, X
 
dc.contributor.authorHuang, W
 
dc.contributor.authorHwa, T
 
dc.contributor.authorHuang, JD
 
dc.date.accessioned2012-05-29T06:05:11Z
 
dc.date.available2012-05-29T06:05:11Z
 
dc.date.issued2011
 
dc.description.abstractPeriodic stripe patterns are ubiquitous in living organisms, yet the underlying developmental processes are complex and difficult to disentangle. We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises from a recurrent aggregation process at the front of the continuously expanding cell population. The number of stripes formed could be tuned by modulating the basal expression of a single gene. The results establish motility control as a simple route to establishing recurrent structures without requiring an extrinsic pacemaker.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationScience, 2011, v. 334 n. 6053, p. 238-241 [How to Cite?]
DOI: http://dx.doi.org/10.1126/science.1209042
 
dc.identifier.citeulike9905436
 
dc.identifier.doihttp://dx.doi.org/10.1126/science.1209042
 
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dc.identifier.isiWOS:000295833600050
Funding AgencyGrant Number
Hong Kong University (HKU)
HKU Committee
Research Grants Council (RGC)HKU1/CRF/10
HKU Faculty of Medicine
NSF through the Center for Theoretical Biological PhysicsPHY-0822283
State Hessen
RGC of the Hong Kong Special Administrative Region201606
Funding Information:

We are grateful to members of the University of Hong Kong Team for The International Genetic Engineering Machine Competition (iGEM) 2008 for their contribution to the project. We thank H. Berg, A. Courey, A. Danchin, D. Smith, J. Tailleur, and C. Voigt for valuable comments. This project was supported by a Hong Kong University (HKU) University Development Fund, a Small Project Grant from the HKU Committee on Research and Conference Grants, and a Collaborative Research Fund from the Research Grants Council (RGC) (HKU1/CRF/10) to J.D.H., and a HKU Faculty of Medicine Development Fund to W.H. TH is supported by the NSF through the Center for Theoretical Biological Physics (grant PHY-0822283) and additionally acknowledges an HKU Distinguished Visiting Professorship. P.L. acknowledges support through the LOEWE program of the State Hessen. L.H.T. acknowledges support by the RGC of the Hong Kong Special Administrative Region under grant 201606. E. coli strains and plasmids are available under a material transfer agreement with the University of Hong Kong.

 
dc.identifier.issn0036-8075
2012 Impact Factor: 31.027
2012 SCImago Journal Rankings: 10.618
 
dc.identifier.issue6053
 
dc.identifier.pmid21998392
 
dc.identifier.scopuseid_2-s2.0-80054124775
 
dc.identifier.spage238
 
dc.identifier.urihttp://hdl.handle.net/10722/147646
 
dc.identifier.volume334
 
dc.languageeng
 
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofScience
 
dc.relation.projectProgramming the Second Generation Tumor-targeting Bacteria
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshAcyl-Butyrolactones - Metabolism
 
dc.subject.meshBacterial Load
 
dc.subject.meshCell Proliferation
 
dc.subject.meshCulture Media
 
dc.subject.meshDiffusion
 
dc.subject.meshEscherichia Coli K12 - Cytology - Genetics - Growth & Development - Physiology
 
dc.subject.meshGene Expression Regulation, Bacterial
 
dc.subject.meshGene Regulatory Networks
 
dc.subject.meshKinetics
 
dc.subject.meshModels, Biological
 
dc.subject.meshMovement
 
dc.subject.meshQuorum Sensing
 
dc.subject.meshSynthetic Biology
 
dc.titleSequential establishment of stripe patterns in an expanding cell population
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Baptist University
  3. Universität Marburg
  4. University of California, San Diego