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Article: Sequential establishment of stripe patterns in an expanding cell population

TitleSequential establishment of stripe patterns in an expanding cell population
Authors
Liu, CFu, XLiu, LRen, XChau, CKLLi, SXiang, LZeng, HChen, GTang, LHLenz, PCui, XHuang, WHwa, THuang, JD
Issue Date2011
PublisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.org
Citation
Science, 2011, v. 334 n. 6053, p. 238-241 How to Cite?
DOI: http://dx.doi.org/10.1126/science.1209042
AbstractPeriodic stripe patterns are ubiquitous in living organisms, yet the underlying developmental processes are complex and difficult to disentangle. We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises from a recurrent aggregation process at the front of the continuously expanding cell population. The number of stripes formed could be tuned by modulating the basal expression of a single gene. The results establish motility control as a simple route to establishing recurrent structures without requiring an extrinsic pacemaker.
Persistent Identifierhttp://hdl.handle.net/10722/147646
ISSN
0036-8075
2023 Impact Factor: 44.7
2023 SCImago Journal Rankings: 11.902
ISI Accession Number ID
WOS:000295833600050
Funding AgencyGrant Number
Hong Kong University (HKU)
HKU Committee
Research Grants Council (RGC)HKU1/CRF/10
HKU Faculty of Medicine
NSF through the Center for Theoretical Biological PhysicsPHY-0822283
State Hessen
RGC of the Hong Kong Special Administrative Region201606
Funding Information:

We are grateful to members of the University of Hong Kong Team for The International Genetic Engineering Machine Competition (iGEM) 2008 for their contribution to the project. We thank H. Berg, A. Courey, A. Danchin, D. Smith, J. Tailleur, and C. Voigt for valuable comments. This project was supported by a Hong Kong University (HKU) University Development Fund, a Small Project Grant from the HKU Committee on Research and Conference Grants, and a Collaborative Research Fund from the Research Grants Council (RGC) (HKU1/CRF/10) to J.D.H., and a HKU Faculty of Medicine Development Fund to W.H. TH is supported by the NSF through the Center for Theoretical Biological Physics (grant PHY-0822283) and additionally acknowledges an HKU Distinguished Visiting Professorship. P.L. acknowledges support through the LOEWE program of the State Hessen. L.H.T. acknowledges support by the RGC of the Hong Kong Special Administrative Region under grant 201606. E. coli strains and plasmids are available under a material transfer agreement with the University of Hong Kong.

References
References in Scopus
Grants
Programming the Second Generation Tumor-targeting Bacteria

 

DC FieldValueLanguage
dc.contributor.authorLiu, Cen_HK
dc.contributor.authorFu, Xen_HK
dc.contributor.authorLiu, Len_HK
dc.contributor.authorRen, Xen_HK
dc.contributor.authorChau, CKLen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorXiang, Len_HK
dc.contributor.authorZeng, Hen_HK
dc.contributor.authorChen, Gen_HK
dc.contributor.authorTang, LHen_HK
dc.contributor.authorLenz, Pen_HK
dc.contributor.authorCui, Xen_HK
dc.contributor.authorHuang, Wen_HK
dc.contributor.authorHwa, Ten_HK
dc.contributor.authorHuang, JDen_HK
dc.date.accessioned2012-05-29T06:05:11Z-
dc.date.available2012-05-29T06:05:11Z-
dc.date.issued2011en_HK
dc.identifier.citationScience, 2011, v. 334 n. 6053, p. 238-241en_HK
dc.identifier.issn0036-8075en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147646-
dc.description.abstractPeriodic stripe patterns are ubiquitous in living organisms, yet the underlying developmental processes are complex and difficult to disentangle. We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises from a recurrent aggregation process at the front of the continuously expanding cell population. The number of stripes formed could be tuned by modulating the basal expression of a single gene. The results establish motility control as a simple route to establishing recurrent structures without requiring an extrinsic pacemaker.en_HK
dc.languageengen_US
dc.publisherAmerican Association for the Advancement of Science. The Journal's web site is located at http://sciencemag.orgen_HK
dc.relation.ispartofScienceen_HK
dc.rightsThis is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on vol. 334, 14 Oct 2011, DOI: 10.1126/science.1209042.-
dc.subject.meshAcyl-Butyrolactones - Metabolismen_US
dc.subject.meshBacterial Loaden_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDiffusionen_US
dc.subject.meshEscherichia Coli K12 - Cytology - Genetics - Growth & Development - Physiologyen_US
dc.subject.meshGene Expression Regulation, Bacterialen_US
dc.subject.meshGene Regulatory Networksen_US
dc.subject.meshKineticsen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshMovementen_US
dc.subject.meshQuorum Sensingen_US
dc.subject.meshSynthetic Biologyen_US
dc.titleSequential establishment of stripe patterns in an expanding cell populationen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, G: ghchen@hku.hken_HK
dc.identifier.emailCui, X: xdcui@hku.hken_HK
dc.identifier.emailHuang, JD: jdhuang@hku.hken_HK
dc.identifier.authorityChen, G=rp00671en_HK
dc.identifier.authorityCui, X=rp00689en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturepostprinten_US
dc.identifier.doi10.1126/science.1209042en_HK
dc.identifier.pmid21998392-
dc.identifier.scopuseid_2-s2.0-80054124775en_HK
dc.identifier.hkuros203767-
dc.identifier.hkuros209359-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80054124775&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume334en_HK
dc.identifier.issue6053en_HK
dc.identifier.spage238en_HK
dc.identifier.epage241en_HK
dc.identifier.eissn1095-9203-
dc.identifier.isiWOS:000295833600050-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f100013985959-
dc.relation.projectProgramming the Second Generation Tumor-targeting Bacteria-
dc.identifier.scopusauthoridLiu, C=54389427600en_HK
dc.identifier.scopusauthoridFu, X=54389038900en_HK
dc.identifier.scopusauthoridLiu, L=54389429500en_HK
dc.identifier.scopusauthoridRen, X=54389742000en_HK
dc.identifier.scopusauthoridChau, CKL=54388825400en_HK
dc.identifier.scopusauthoridLi, S=54389684300en_HK
dc.identifier.scopusauthoridXiang, L=54390349600en_HK
dc.identifier.scopusauthoridZeng, H=24466915800en_HK
dc.identifier.scopusauthoridChen, G=35253368600en_HK
dc.identifier.scopusauthoridTang, LH=7402081089en_HK
dc.identifier.scopusauthoridLenz, P=7103014121en_HK
dc.identifier.scopusauthoridCui, X=10839907500en_HK
dc.identifier.scopusauthoridHuang, W=14123326400en_HK
dc.identifier.scopusauthoridHwa, T=7003505824en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.citeulike9905436-
dc.identifier.issnl0036-8075-

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