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Article: Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1.

TitleRaf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1.
Authors
Issue Date2010
Citation
Methods In Molecular Biology (Clifton, N.J.), 2010, v. 647, p. 113-123 How to Cite?
AbstractThe proliferation-associated transcription factor FOXM1 is essential for cell cycle progression into mitosis. Using synchronized human fibroblasts we detected, by immunostaining, that FOXM1 is localized predominantly in the cytoplasm in cells at late-G1 and S phases. Nuclear translocation occurs just before progression into the G2/M phase of the cell cycle and requires activity of the Raf/MEK/MAPK signaling pathway. Using pharmacological modulators, we demonstrated that activity of the Raf/MEK/MAPK pathway is both necessary and sufficient for the nuclear translocation of FOXM1. Consistent with FoxM1c being the major isoform expressed in proliferating fibroblasts, constitutively active MEK1 enhances the transactivating effect of FOXM1c, but not FOXM1b, on the cyclin B1 promoter in transient reporter assays. Here, we describe in detail the methods involved in generating these findings, which support the notion that FOXM1 is an effector of Raf/MEK/MAPK signaling in G2/M regulation.
Persistent Identifierhttp://hdl.handle.net/10722/147628
ISSN

 

DC FieldValueLanguage
dc.contributor.authorMa, RYen_US
dc.contributor.authorTong, THen_US
dc.contributor.authorLeung, WYen_US
dc.contributor.authorYao, KMen_US
dc.date.accessioned2012-05-29T06:05:05Z-
dc.date.available2012-05-29T06:05:05Z-
dc.date.issued2010en_US
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2010, v. 647, p. 113-123en_US
dc.identifier.issn1940-6029en_US
dc.identifier.urihttp://hdl.handle.net/10722/147628-
dc.description.abstractThe proliferation-associated transcription factor FOXM1 is essential for cell cycle progression into mitosis. Using synchronized human fibroblasts we detected, by immunostaining, that FOXM1 is localized predominantly in the cytoplasm in cells at late-G1 and S phases. Nuclear translocation occurs just before progression into the G2/M phase of the cell cycle and requires activity of the Raf/MEK/MAPK signaling pathway. Using pharmacological modulators, we demonstrated that activity of the Raf/MEK/MAPK pathway is both necessary and sufficient for the nuclear translocation of FOXM1. Consistent with FoxM1c being the major isoform expressed in proliferating fibroblasts, constitutively active MEK1 enhances the transactivating effect of FOXM1c, but not FOXM1b, on the cyclin B1 promoter in transient reporter assays. Here, we describe in detail the methods involved in generating these findings, which support the notion that FOXM1 is an effector of Raf/MEK/MAPK signaling in G2/M regulation.en_US
dc.languageengen_US
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_US
dc.subject.meshActive Transport, Cell Nucleus - Drug Effectsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Nucleus - Drug Effects - Metabolismen_US
dc.subject.meshFibroblasts - Metabolismen_US
dc.subject.meshForkhead Transcription Factors - Metabolismen_US
dc.subject.meshGenes, Reporter - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMap Kinase Signaling System - Drug Effectsen_US
dc.subject.meshMiceen_US
dc.subject.meshMitogen-Activated Protein Kinase Kinases - Metabolismen_US
dc.subject.meshTranscriptional Activation - Drug Effectsen_US
dc.subject.meshRaf Kinases - Metabolismen_US
dc.titleRaf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1.en_US
dc.typeArticleen_US
dc.identifier.emailYao, KM:kmyao@hku.hken_US
dc.identifier.authorityYao, KM=rp00344en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid20694663-
dc.identifier.scopuseid_2-s2.0-79952113188en_US
dc.identifier.volume647en_US
dc.identifier.spage113en_US
dc.identifier.epage123en_US
dc.identifier.scopusauthoridMa, RY=8323783700en_US
dc.identifier.scopusauthoridTong, TH=8323783200en_US
dc.identifier.scopusauthoridLeung, WY=7201504543en_US
dc.identifier.scopusauthoridYao, KM=7403234578en_US

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