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Article: Utility of Hoxb2 enhancer-mediated Cre activity for functional studies in the developing inner ear

TitleUtility of Hoxb2 enhancer-mediated Cre activity for functional studies in the developing inner ear
Authors
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/68503812
Citation
Genesis, 2009, v. 47 n. 6, p. 361-365 How to Cite?
AbstractThe rhombomere 4(r4)-restricted expression of the mouse Hoxb2 gene is regulated by a 1.4-kb enhancer-containing fragment. Here,we show that transgenic mouse lines expressing cre driven by this fragment (B2-r4-Cre), activated the R26R Cre reporter in rhombomere 4 and the second branchial arch, the epithelium of the first branchial arch, apical ectodermal ridge of the limb buds and the tail region. Of particular interest is Cre activity in the developing inner ear. Cre activity was found in the preotic field and otic placode at E8.5 and otocyst at E9.5-E12.5, in the cochleovestibular and facio-acoustic ganglia at E10.5 and the vestibular and spiral ganglia and all the otic epithelia derived from the otocyst at E15.5 and P0. Our data suggest that the B2-r4-Cre transgenic mice provide an important tool for conditional gene manipulation and lineage tracing in the inner ear. In combination with other transgenic lines expressing cre exclusively in the otic vesicle, the relative contributions of the hindbrain, periotic mesenchyme and otic epithelium in otic development can be dissected. © 2009 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/147610
ISSN
2015 Impact Factor: 2.165
2015 SCImago Journal Rankings: 1.324
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants, Council of the Hong Kong Special Administrative Region, ChinaHKU2/01C
HKU4/05C
University Research Committee, University of Hong Kong
Funding Information:

Contract grant sponsor: Research Grants, Council of the Hong Kong Special Administrative Region, China; Contract grant number: HKU2/01C, HKU4/05C; Contract grant sponsor: University Research Committee, University of Hong Kong

References

 

DC FieldValueLanguage
dc.contributor.authorSzeto, IYYen_US
dc.contributor.authorLeung, KKHen_US
dc.contributor.authorSham, MHen_US
dc.contributor.authorCheah, KSEen_US
dc.date.accessioned2012-05-29T06:04:58Z-
dc.date.available2012-05-29T06:04:58Z-
dc.date.issued2009en_US
dc.identifier.citationGenesis, 2009, v. 47 n. 6, p. 361-365en_US
dc.identifier.issn1526-954Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/147610-
dc.description.abstractThe rhombomere 4(r4)-restricted expression of the mouse Hoxb2 gene is regulated by a 1.4-kb enhancer-containing fragment. Here,we show that transgenic mouse lines expressing cre driven by this fragment (B2-r4-Cre), activated the R26R Cre reporter in rhombomere 4 and the second branchial arch, the epithelium of the first branchial arch, apical ectodermal ridge of the limb buds and the tail region. Of particular interest is Cre activity in the developing inner ear. Cre activity was found in the preotic field and otic placode at E8.5 and otocyst at E9.5-E12.5, in the cochleovestibular and facio-acoustic ganglia at E10.5 and the vestibular and spiral ganglia and all the otic epithelia derived from the otocyst at E15.5 and P0. Our data suggest that the B2-r4-Cre transgenic mice provide an important tool for conditional gene manipulation and lineage tracing in the inner ear. In combination with other transgenic lines expressing cre exclusively in the otic vesicle, the relative contributions of the hindbrain, periotic mesenchyme and otic epithelium in otic development can be dissected. © 2009 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/68503812en_US
dc.relation.ispartofGenesisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshEar, Inner - Cytology - Embryology - Metabolismen_US
dc.subject.meshEnhancer Elements, Genetic - Geneticsen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshGenetic Techniquesen_US
dc.subject.meshHomeodomain Proteins - Geneticsen_US
dc.subject.meshIntegrases - Genetics - Metabolismen_US
dc.subject.meshLac Operon - Geneticsen_US
dc.subject.meshMesoderm - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Strainsen_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshRhombencephalon - Embryology - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTranscription Factors - Geneticsen_US
dc.subject.meshBeta-Galactosidase - Metabolismen_US
dc.titleUtility of Hoxb2 enhancer-mediated Cre activity for functional studies in the developing inner earen_US
dc.typeArticleen_US
dc.identifier.emailLeung, KKH:keithlee@hku.hken_US
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_US
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_US
dc.identifier.authorityLeung, KKH=rp00298en_US
dc.identifier.authoritySham, MH=rp00380en_US
dc.identifier.authorityCheah, KSE=rp00342en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/dvg.20507en_US
dc.identifier.pmid19370753-
dc.identifier.scopuseid_2-s2.0-70349179475en_US
dc.identifier.hkuros157185-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349179475&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume47en_US
dc.identifier.issue6en_US
dc.identifier.spage361en_US
dc.identifier.epage365en_US
dc.identifier.isiWOS:000267352400001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSzeto, IYY=6602491785en_US
dc.identifier.scopusauthoridLeung, KKH=7401860467en_US
dc.identifier.scopusauthoridSham, MH=7003729109en_US
dc.identifier.scopusauthoridCheah, KSE=35387746200en_US

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