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Article: Identification of sperm-binding proteins in the ventral prostate of the golden hamster

TitleIdentification of sperm-binding proteins in the ventral prostate of the golden hamster
Authors
Issue Date2007
Citation
Zhonghua Nan Ke Xue = National Journal Of Andrology, 2007, v. 13 n. 10, p. 883-889 How to Cite?
AbstractOBJECTIVE: To investigate the binding of secretory proteins in the ventral prostate to the surface of sperm. METHODS: We used different techniques to demonstrate the possibility of ventral prostate secretory proteins binding to sperm in golden hamsters. Polyclonal antibodies against crude secretion of the ventral prostate cultured in rabbits were used to detect the antigens in hamster epididymal, uterine and oviductal spermatozoa by indirect immunofluorescence technique. The uterine and oviductal spermatozoa were collected after mating with the males with or without ventral prostate glands. The ventral prostate secretory proteins were isolated and transblotted to the membrane, which was incubated with the biotinylated epididymal sperm membrane proteins, and then the biotinylated binding proteins were stained. RESULTS: An immunoreaction restricted to the middle piece was observed in the sperm incubated with the ventral prostate secretion and ejaculated sperm recovered from the uteri and oviducts. The rate of the epididymal sperm bound with the ventral prostate secretory proteins was (80 +/- 5) %, and the rats of the sperm binding to the ventral prostate secretory proteins were (30.0 +/- 4.6) % from the uterus and (16.0 +/- 3.6) % from the oviduct after mating with the males with ventral prostate glands, significantly higher than after mating with those without prostate glands (P < 0.01). Five bands were identified by Western blot analysis in vitro of the ventral prostate secretory proteins incubated with biotinylated epididymal sperm membrane proteins. CONCLUSION: The present data indicate that ventral prostate secretory proteins bind to the middle piece of sperm in golden hamsters.
Persistent Identifierhttp://hdl.handle.net/10722/147591
ISSN
2015 SCImago Journal Rankings: 0.128

 

DC FieldValueLanguage
dc.contributor.authorLuo, JMen_HK
dc.contributor.authorCheng, Len_HK
dc.contributor.authorZhou, YCen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorChow, PHen_HK
dc.date.accessioned2012-05-29T06:04:49Z-
dc.date.available2012-05-29T06:04:49Z-
dc.date.issued2007en_HK
dc.identifier.citationZhonghua Nan Ke Xue = National Journal Of Andrology, 2007, v. 13 n. 10, p. 883-889en_HK
dc.identifier.issn1009-3591en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147591-
dc.description.abstractOBJECTIVE: To investigate the binding of secretory proteins in the ventral prostate to the surface of sperm. METHODS: We used different techniques to demonstrate the possibility of ventral prostate secretory proteins binding to sperm in golden hamsters. Polyclonal antibodies against crude secretion of the ventral prostate cultured in rabbits were used to detect the antigens in hamster epididymal, uterine and oviductal spermatozoa by indirect immunofluorescence technique. The uterine and oviductal spermatozoa were collected after mating with the males with or without ventral prostate glands. The ventral prostate secretory proteins were isolated and transblotted to the membrane, which was incubated with the biotinylated epididymal sperm membrane proteins, and then the biotinylated binding proteins were stained. RESULTS: An immunoreaction restricted to the middle piece was observed in the sperm incubated with the ventral prostate secretion and ejaculated sperm recovered from the uteri and oviducts. The rate of the epididymal sperm bound with the ventral prostate secretory proteins was (80 +/- 5) %, and the rats of the sperm binding to the ventral prostate secretory proteins were (30.0 +/- 4.6) % from the uterus and (16.0 +/- 3.6) % from the oviduct after mating with the males with ventral prostate glands, significantly higher than after mating with those without prostate glands (P < 0.01). Five bands were identified by Western blot analysis in vitro of the ventral prostate secretory proteins incubated with biotinylated epididymal sperm membrane proteins. CONCLUSION: The present data indicate that ventral prostate secretory proteins bind to the middle piece of sperm in golden hamsters.en_HK
dc.languageengen_US
dc.relation.ispartofZhonghua nan ke xue = National journal of andrologyen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshEpididymis - Metabolismen_US
dc.subject.meshFallopian Tubes - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFluorescent Antibody Technique, Indirecten_US
dc.subject.meshMaleen_US
dc.subject.meshMesocricetusen_US
dc.subject.meshProstate - Metabolismen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshSeminal Vesicle Secretory Proteins - Metabolismen_US
dc.subject.meshSpermatozoa - Metabolismen_US
dc.subject.meshUterus - Metabolismen_US
dc.titleIdentification of sperm-binding proteins in the ventral prostate of the golden hamsteren_HK
dc.typeArticleen_HK
dc.identifier.emailCheng, L:lcheng@hkucc.hku.hken_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityCheng, L=rp00317en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid17977318-
dc.identifier.scopuseid_2-s2.0-55249121244en_HK
dc.identifier.volume13en_HK
dc.identifier.issue10en_HK
dc.identifier.spage883en_HK
dc.identifier.epage889en_HK
dc.identifier.scopusauthoridLuo, JM=8780499000en_HK
dc.identifier.scopusauthoridCheng, L=7403337122en_HK
dc.identifier.scopusauthoridZhou, YC=24179107900en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK

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