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Article: The forkhead box M1 protein regulates the transcription of the estrogen receptor α in breast cancer cells

TitleThe forkhead box M1 protein regulates the transcription of the estrogen receptor α in breast cancer cells
Authors
Issue Date2006
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2006, v. 281 n. 35, p. 25167-25176 How to Cite?
AbstractIn this study, we have identified the Forkhead transcription factor FoxM1 as a physiological regulator of estrogen receptor α (ERα) expression in breast carcinoma cells. Our survey of a panel of 16 different breast cell lines showed a good correlation (13/16) between FoxM1 expression and expression of ERα at both protein and mRNA levels. We have also demonstrated that ectopic expression of FoxM1 in two different estrogen receptor-positive breast cancer cell lines, MCF-7 and ZR-75-30, led to up-regulation of ERα expression at protein and transcript levels. Furthermore, treatment of MCF-7 cells with the MEK inhibitor U0126, which blocks ERK1/2-dependent activation of FoxM1, also repressed ERα expression. Consistent with this, silencing of FoxM1 expression in MCF-7 cells using small interfering RNA resulted in the almost complete abrogation of ERα expression. We also went on to show that FoxM1 can activate the transcriptional activity of human ERα promoter primarily through two closely located Forkhead response elements located at the proximal region of the ERα promoter. Chromatin immunoprecipitation and biotinylated oligonucleotide pulldown assays have allowed us to confirm these Forkhead response elements as important for FoxM1 binding. Further co-immunoprecipitation experiments showed that FoxO3a and FoxM1 interact in vivo. Together with the chromatin immunoprecipitation and biotinylated oligonucleotide pulldown data, the co-immunoprecipitation results also suggest the possibility that FoxM1 and FoxO3a cooperate to regulate ERα gene transcription. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/147547
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMadureira, PAen_US
dc.contributor.authorVarshochi, Ren_US
dc.contributor.authorConstantinidou, Den_US
dc.contributor.authorFrancis, REen_US
dc.contributor.authorCoombes, RCen_US
dc.contributor.authorYao, KMen_US
dc.contributor.authorLam, EWFen_US
dc.date.accessioned2012-05-29T06:04:30Z-
dc.date.available2012-05-29T06:04:30Z-
dc.date.issued2006en_US
dc.identifier.citationJournal Of Biological Chemistry, 2006, v. 281 n. 35, p. 25167-25176en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/147547-
dc.description.abstractIn this study, we have identified the Forkhead transcription factor FoxM1 as a physiological regulator of estrogen receptor α (ERα) expression in breast carcinoma cells. Our survey of a panel of 16 different breast cell lines showed a good correlation (13/16) between FoxM1 expression and expression of ERα at both protein and mRNA levels. We have also demonstrated that ectopic expression of FoxM1 in two different estrogen receptor-positive breast cancer cell lines, MCF-7 and ZR-75-30, led to up-regulation of ERα expression at protein and transcript levels. Furthermore, treatment of MCF-7 cells with the MEK inhibitor U0126, which blocks ERK1/2-dependent activation of FoxM1, also repressed ERα expression. Consistent with this, silencing of FoxM1 expression in MCF-7 cells using small interfering RNA resulted in the almost complete abrogation of ERα expression. We also went on to show that FoxM1 can activate the transcriptional activity of human ERα promoter primarily through two closely located Forkhead response elements located at the proximal region of the ERα promoter. Chromatin immunoprecipitation and biotinylated oligonucleotide pulldown assays have allowed us to confirm these Forkhead response elements as important for FoxM1 binding. Further co-immunoprecipitation experiments showed that FoxO3a and FoxM1 interact in vivo. Together with the chromatin immunoprecipitation and biotinylated oligonucleotide pulldown data, the co-immunoprecipitation results also suggest the possibility that FoxM1 and FoxO3a cooperate to regulate ERα gene transcription. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshBiotinylationen_US
dc.subject.meshBreast Neoplasms - Metabolismen_US
dc.subject.meshCell Cycleen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshEstrogen Receptor Alpha - Biosynthesis - Geneticsen_US
dc.subject.meshForkhead Transcription Factors - Chemistry - Physiologyen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshOligonucleotides - Chemistryen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshUp-Regulationen_US
dc.titleThe forkhead box M1 protein regulates the transcription of the estrogen receptor α in breast cancer cellsen_US
dc.typeArticleen_US
dc.identifier.emailYao, KM:kmyao@hku.hken_US
dc.identifier.authorityYao, KM=rp00344en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1074/jbc.M603906200en_US
dc.identifier.pmid16809346-
dc.identifier.scopuseid_2-s2.0-33748755657en_US
dc.identifier.hkuros129003-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748755657&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume281en_US
dc.identifier.issue35en_US
dc.identifier.spage25167en_US
dc.identifier.epage25176en_US
dc.identifier.eissn1083-351X-
dc.identifier.isiWOS:000240031300017-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMadureira, PA=10340140500en_US
dc.identifier.scopusauthoridVarshochi, R=6506468719en_US
dc.identifier.scopusauthoridConstantinidou, D=6507606383en_US
dc.identifier.scopusauthoridFrancis, RE=14621377000en_US
dc.identifier.scopusauthoridCoombes, RC=7202546957en_US
dc.identifier.scopusauthoridYao, KM=7403234578en_US
dc.identifier.scopusauthoridLam, EWF=7102889877en_US

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