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Article: Localization of nerve growth factor, neurotrophin-3, and glial cell line-derived neurotrophic factor in nestin-expressing reactive astrocytes in the caudate-putamen of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated C57/B1 mice

TitleLocalization of nerve growth factor, neurotrophin-3, and glial cell line-derived neurotrophic factor in nestin-expressing reactive astrocytes in the caudate-putamen of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated C57/B1 mice
Authors
KeywordsBasal ganglia
Nestin
Neuroprotection
Neurotrophins
Parkinson's disease
Reactive astrocytes
Issue Date2006
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal of Comparative Neurology, 2006, v. 497 n. 6, p. 898-909 How to Cite?
AbstractTo address the hypothesis that reactive astrocytes in the basal ganglia of an animal model of Parkinson's disease serve neurotrophic roles, we studied the expression pattern of neurotrophic factors in the basal ganglia of C57/B1 mice that had been treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to induce the degeneration of nigral dopamine neurons and parkinsonism. MPTP induced significant neuronal degeneration in the substantia nigra pars compacta as detected with Fluoro-Jade B staining, and this was accompanied by an increase in nestin-expressing astrocytes within the caudate-putamen. The number of nestin-positive reactive astrocytes in the caudate-putamen peaked within 3-5 days following MPTP treatment and then declined progressively toward the basal level by 21 days after treatment. Immunofluorescence and confocal microscopy confirmed coexpression of nestin or Ki-67 (cell proliferation marker) in glial fibrillary acid protein-positive astrocytes in the caudate-putamen. Double immunolabeling further revealed immunoreactivities for nerve growth factor (NGF), neurotrophin-3 (NTS), and glial cell line-derived neurotrophic factor (GDNF) in nestin-positive reactive astrocytes. Semi-quantification of data obtained from mice 5 days after MPTP injection indicated that the majority of nestin-expressing cells expressed NGF (92%), NT3 (90%), or GDNF (86%). Our results present novel evidence of neurotrophic features among reactive astrocytes in the dopamine-depleted striatum. These nestin-expressing reactive astrocytes may therefore play neurotrophic roles in neural remodeling of the basal ganglia in Parkinson's disease. © 2006 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/147539
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, LWen_HK
dc.contributor.authorZhang, JPen_HK
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2012-05-29T06:04:27Z-
dc.date.available2012-05-29T06:04:27Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal of Comparative Neurology, 2006, v. 497 n. 6, p. 898-909en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147539-
dc.description.abstractTo address the hypothesis that reactive astrocytes in the basal ganglia of an animal model of Parkinson's disease serve neurotrophic roles, we studied the expression pattern of neurotrophic factors in the basal ganglia of C57/B1 mice that had been treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to induce the degeneration of nigral dopamine neurons and parkinsonism. MPTP induced significant neuronal degeneration in the substantia nigra pars compacta as detected with Fluoro-Jade B staining, and this was accompanied by an increase in nestin-expressing astrocytes within the caudate-putamen. The number of nestin-positive reactive astrocytes in the caudate-putamen peaked within 3-5 days following MPTP treatment and then declined progressively toward the basal level by 21 days after treatment. Immunofluorescence and confocal microscopy confirmed coexpression of nestin or Ki-67 (cell proliferation marker) in glial fibrillary acid protein-positive astrocytes in the caudate-putamen. Double immunolabeling further revealed immunoreactivities for nerve growth factor (NGF), neurotrophin-3 (NTS), and glial cell line-derived neurotrophic factor (GDNF) in nestin-positive reactive astrocytes. Semi-quantification of data obtained from mice 5 days after MPTP injection indicated that the majority of nestin-expressing cells expressed NGF (92%), NT3 (90%), or GDNF (86%). Our results present novel evidence of neurotrophic features among reactive astrocytes in the dopamine-depleted striatum. These nestin-expressing reactive astrocytes may therefore play neurotrophic roles in neural remodeling of the basal ganglia in Parkinson's disease. © 2006 Wiley-Liss, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subjectBasal gangliaen_HK
dc.subjectNestinen_HK
dc.subjectNeuroprotectionen_HK
dc.subjectNeurotrophinsen_HK
dc.subjectParkinson's diseaseen_HK
dc.subjectReactive astrocytesen_HK
dc.subject.mesh1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAstrocytes - Chemistry - Drug Effects - Metabolismen_US
dc.subject.meshCaudate Nucleus - Chemistry - Drug Effects - Metabolismen_US
dc.subject.meshGlial Cell Line-Derived Neurotrophic Factors - Analysis - Metabolismen_US
dc.subject.meshIntermediate Filament Proteins - Biosynthesisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshNeostriatum - Chemistry - Drug Effects - Metabolismen_US
dc.subject.meshNerve Growth Factor - Analysis - Metabolismen_US
dc.subject.meshNerve Tissue Proteins - Biosynthesisen_US
dc.subject.meshNeurotrophin 3 - Analysis - Metabolismen_US
dc.subject.meshPutamen - Chemistry - Drug Effects - Metabolismen_US
dc.titleLocalization of nerve growth factor, neurotrophin-3, and glial cell line-derived neurotrophic factor in nestin-expressing reactive astrocytes in the caudate-putamen of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated C57/B1 miceen_HK
dc.typeArticleen_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/cne.21014en_HK
dc.identifier.pmid16802332-
dc.identifier.scopuseid_2-s2.0-33745883258en_HK
dc.identifier.hkuros121711-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745883258&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume497en_HK
dc.identifier.issue6en_HK
dc.identifier.spage898en_HK
dc.identifier.epage909en_HK
dc.identifier.isiWOS:000239110900004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, LW=7409444941en_HK
dc.identifier.scopusauthoridZhang, JP=14030623900en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

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