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Article: Identification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase

TitleIdentification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase
Authors
KeywordsDNase I footprinting
Eggplant
EMSA
Ethylene-responsive element
Evening element
Promoter analysis
Issue Date2005
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 2005, v. 57 n. 5, p. 629-643 How to Cite?
AbstractWe have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5′-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions and by in vitro binding assays. Analysis of transgenic tobacco transformed with SmCP promoter-GUS constructs confirmed that the promoter region -415/+54 containing Ethylene Responsive Element ERE(-355/-348) conferred threefold ethylene-induction of GUS expression, while -827/+54 which also contains ERE(-683/-676), produced fivefold induction. Using gel mobility shift assays, we demonstrated that each ERE binds nuclear proteins from both ethephon-treated and untreated 5-week-old seedlings, suggesting that different transcriptions factors bind each ERE under varying physiological conditions. Binding was also observed in extracts from senescent, but not young, fruits. The variation in binding at the EREs in fruits and seedlings imply that organ-specific factors may participate in binding. Analysis of transgenic tobacco expressing various SmCP promoter-lucconstructs containing wild-type or mutant Evening Elements (EEs) confirmed that both conserved EEs at -795/-787 and -785/-777 are important in circadian control. We confirmed the binding of total nuclear proteins to EEs in gel mobility shift assays and in DNase I footprinting. Our results suggest that multiple proteins bind the EEs which are conserved in plants other thanArabidopsisand that functional EEs and EREs are present in the 5′-flanking region of a gene encoding cysteine proteinase. © Springer 2005.
Persistent Identifierhttp://hdl.handle.net/10722/147518
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRawat, Ren_HK
dc.contributor.authorXu, ZFen_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2012-05-29T06:04:18Z-
dc.date.available2012-05-29T06:04:18Z-
dc.date.issued2005en_HK
dc.identifier.citationPlant Molecular Biology, 2005, v. 57 n. 5, p. 629-643en_HK
dc.identifier.issn0167-4412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147518-
dc.description.abstractWe have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5′-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions and by in vitro binding assays. Analysis of transgenic tobacco transformed with SmCP promoter-GUS constructs confirmed that the promoter region -415/+54 containing Ethylene Responsive Element ERE(-355/-348) conferred threefold ethylene-induction of GUS expression, while -827/+54 which also contains ERE(-683/-676), produced fivefold induction. Using gel mobility shift assays, we demonstrated that each ERE binds nuclear proteins from both ethephon-treated and untreated 5-week-old seedlings, suggesting that different transcriptions factors bind each ERE under varying physiological conditions. Binding was also observed in extracts from senescent, but not young, fruits. The variation in binding at the EREs in fruits and seedlings imply that organ-specific factors may participate in binding. Analysis of transgenic tobacco expressing various SmCP promoter-lucconstructs containing wild-type or mutant Evening Elements (EEs) confirmed that both conserved EEs at -795/-787 and -785/-777 are important in circadian control. We confirmed the binding of total nuclear proteins to EEs in gel mobility shift assays and in DNase I footprinting. Our results suggest that multiple proteins bind the EEs which are conserved in plants other thanArabidopsisand that functional EEs and EREs are present in the 5′-flanking region of a gene encoding cysteine proteinase. © Springer 2005.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_HK
dc.relation.ispartofPlant Molecular Biologyen_HK
dc.subjectDNase I footprintingen_HK
dc.subjectEggplanten_HK
dc.subjectEMSAen_HK
dc.subjectEthylene-responsive elementen_HK
dc.subjectEvening elementen_HK
dc.subjectPromoter analysisen_HK
dc.subject.mesh5' Flanking Region - Geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sites - Geneticsen_US
dc.subject.meshCircadian Rhythm - Physiologyen_US
dc.subject.meshCysteine Endopeptidases - Geneticsen_US
dc.subject.meshDna Footprinting - Methodsen_US
dc.subject.meshDeoxyribonuclease I - Metabolismen_US
dc.subject.meshElectrophoretic Mobility Shift Assayen_US
dc.subject.meshEthylenes - Pharmacologyen_US
dc.subject.meshGlucuronidase - Genetics - Metabolismen_US
dc.subject.meshLuciferases - Genetics - Metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNuclear Proteins - Metabolismen_US
dc.subject.meshOrganophosphorus Compounds - Pharmacologyen_US
dc.subject.meshPlant Growth Regulators - Pharmacologyen_US
dc.subject.meshPlant Proteins - Geneticsen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshPromoter Regions, Genetic - Geneticsen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRecombinant Fusion Proteins - Genetics - Metabolismen_US
dc.subject.meshResponse Elements - Geneticsen_US
dc.subject.meshSolanum Melongena - Drug Effects - Genetics - Growth & Developmenten_US
dc.subject.meshTobacco - Geneticsen_US
dc.titleIdentification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinaseen_HK
dc.typeArticleen_HK
dc.identifier.emailYao, KM: kmyao@hku.hken_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11103-005-0954-7en_HK
dc.identifier.pmid15988560-
dc.identifier.scopuseid_2-s2.0-21744433013en_HK
dc.identifier.hkuros100178-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21744433013&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume57en_HK
dc.identifier.issue5en_HK
dc.identifier.spage629en_HK
dc.identifier.epage643en_HK
dc.identifier.isiWOS:000231220400002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridRawat, R=8525546500en_HK
dc.identifier.scopusauthoridXu, ZF=7405425506en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

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