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Article: The coactivator Bridge-1 increases transcriptional activation by pancreas duodenum homeobox-1 (PDX-1)

TitleThe coactivator Bridge-1 increases transcriptional activation by pancreas duodenum homeobox-1 (PDX-1)
Authors
Issue Date2005
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mce
Citation
Molecular And Cellular Endocrinology, 2005, v. 237 n. 1-2, p. 67-74 How to Cite?
AbstractWell-orchestrated transcriptional regulation of pancreatic beta cells is essential for insulin production and glucose homeostasis. Pancreas duodenum homeobox-1 (PDX-1) is a key regulator of glucose-dependent insulin production and glucose metabolism. We find that PDX-1 interacts with the PDZ-domain coactivator Bridge-1 in yeast interaction trap assays. Rat Bridge-1 and PDX-1 interact directly in GST pull-down assays via Bridge-1 interactions with the amino-terminal transactivation domain of PDX-1. Bridge-1 also interacts with wild-type and mutant human PDX-1 (IPF-1) proteins and strongly interacts with the amino-terminal PDX-1 P63fsdelC (MODY4) mutant protein. Transcriptional activation by PDX-1 is increased by addition of Bridge-1 in multiple contexts, including synergistic activation of a Gal4 reporter by Gal4-Bridge-1 and Gal4-PDX-1 fusion proteins, activation of the somatostatin promoter TAAT1 enhancer, and synergistic activation of the rat insulin I promoter FarFlat enhancer by PDX-1, E12, and E47. We propose that the coactivator Bridge-1 modulates PDX-1 functions in the regulation of its target genes. © 2005 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/147515
ISSN
2015 Impact Factor: 3.859
2015 SCImago Journal Rankings: 2.116
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorStanojevic, Ven_US
dc.contributor.authorYao, KMen_US
dc.contributor.authorThomas, MKen_US
dc.date.accessioned2012-05-29T06:04:16Z-
dc.date.available2012-05-29T06:04:16Z-
dc.date.issued2005en_US
dc.identifier.citationMolecular And Cellular Endocrinology, 2005, v. 237 n. 1-2, p. 67-74en_US
dc.identifier.issn0303-7207en_US
dc.identifier.urihttp://hdl.handle.net/10722/147515-
dc.description.abstractWell-orchestrated transcriptional regulation of pancreatic beta cells is essential for insulin production and glucose homeostasis. Pancreas duodenum homeobox-1 (PDX-1) is a key regulator of glucose-dependent insulin production and glucose metabolism. We find that PDX-1 interacts with the PDZ-domain coactivator Bridge-1 in yeast interaction trap assays. Rat Bridge-1 and PDX-1 interact directly in GST pull-down assays via Bridge-1 interactions with the amino-terminal transactivation domain of PDX-1. Bridge-1 also interacts with wild-type and mutant human PDX-1 (IPF-1) proteins and strongly interacts with the amino-terminal PDX-1 P63fsdelC (MODY4) mutant protein. Transcriptional activation by PDX-1 is increased by addition of Bridge-1 in multiple contexts, including synergistic activation of a Gal4 reporter by Gal4-Bridge-1 and Gal4-PDX-1 fusion proteins, activation of the somatostatin promoter TAAT1 enhancer, and synergistic activation of the rat insulin I promoter FarFlat enhancer by PDX-1, E12, and E47. We propose that the coactivator Bridge-1 modulates PDX-1 functions in the regulation of its target genes. © 2005 Elsevier Ireland Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/mceen_US
dc.relation.ispartofMolecular and Cellular Endocrinologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDna-Binding Proteins - Genetics - Metabolismen_US
dc.subject.meshHmgb Proteins - Genetics - Metabolismen_US
dc.subject.meshHomeodomain Proteins - Genetics - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProtein Interaction Mappingen_US
dc.subject.meshRatsen_US
dc.subject.meshSaccharomyces Cerevisiaeen_US
dc.subject.meshSomatostatin - Geneticsen_US
dc.subject.meshTcf Transcription Factorsen_US
dc.subject.meshTrans-Activators - Genetics - Metabolismen_US
dc.subject.meshTranscription Factor 7-Like 1 Proteinen_US
dc.subject.meshTranscription Factors - Genetics - Metabolismen_US
dc.subject.meshTranscriptional Activationen_US
dc.titleThe coactivator Bridge-1 increases transcriptional activation by pancreas duodenum homeobox-1 (PDX-1)en_US
dc.typeArticleen_US
dc.identifier.emailYao, KM:kmyao@hku.hken_US
dc.identifier.authorityYao, KM=rp00344en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.mce.2005.03.003en_US
dc.identifier.pmid15885879-
dc.identifier.scopuseid_2-s2.0-19544394114en_US
dc.identifier.hkuros98966-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-19544394114&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume237en_US
dc.identifier.issue1-2en_US
dc.identifier.spage67en_US
dc.identifier.epage74en_US
dc.identifier.isiWOS:000230040400007-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridStanojevic, V=6603050702en_US
dc.identifier.scopusauthoridYao, KM=7403234578en_US
dc.identifier.scopusauthoridThomas, MK=7404754193en_US

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