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Article: Chondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury.

TitleChondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury.
Authors
Issue Date2004
Citation
The Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology, 2004, v. 18 n. 1, p. 194-196 How to Cite?
AbstractGrafting of Schwann cell-seeded channels into hemisected adult rat thoracic spinal cords has been tested as a strategy to bridge the injured cord. Despite success in guiding axonal growth into the graft, regeneration across the distal graft-host interface into the host spinal cord was limited. We hypothesized that chondroitin sulfate (CS) glycoforms deposited at the gliotic front of the interface constitute a molecular barrier to axonal growth into the host cord. Because CS glycoforms deposited by purified astrocytes in vitro were removable by digestion with chondroitinase ABC, we attempted to achieve likewise by infusion of the enzyme to the host side of the interface. By 1 month post-treatment, significant numbers of regenerating axons crossed an interface that was subdued in macrophage/microglia reaction and decreased in CS-immunopositivity. The axons extended as far into the caudal cord as 5 mm, in contrast to nil in vehicle-infused controls. Fascicular organizations of axon-Schwann cell units within the regenerated tissue cable were better-preserved in enzyme-treated cords than in vehicle-infused controls. We conclude that CS glycoforms deposited during gliosis at the distal graft-host interface could be cleared by the in vivo action of chondroitinase ABC to improve prospects of axonal regeneration into the host spinal cord.
Persistent Identifierhttp://hdl.handle.net/10722/147503
ISSN
2015 SCImago Journal Rankings: 2.775
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChau, CHen_HK
dc.contributor.authorShum, DKen_HK
dc.contributor.authorLi, Hen_HK
dc.contributor.authorPei, Jen_HK
dc.contributor.authorLui, YYen_HK
dc.contributor.authorWirthlin, Len_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorXu, XMen_HK
dc.date.accessioned2012-05-29T06:04:11Z-
dc.date.available2012-05-29T06:04:11Z-
dc.date.issued2004en_HK
dc.identifier.citationThe Faseb Journal : Official Publication Of The Federation Of American Societies For Experimental Biology, 2004, v. 18 n. 1, p. 194-196en_HK
dc.identifier.issn1530-6860en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147503-
dc.description.abstractGrafting of Schwann cell-seeded channels into hemisected adult rat thoracic spinal cords has been tested as a strategy to bridge the injured cord. Despite success in guiding axonal growth into the graft, regeneration across the distal graft-host interface into the host spinal cord was limited. We hypothesized that chondroitin sulfate (CS) glycoforms deposited at the gliotic front of the interface constitute a molecular barrier to axonal growth into the host cord. Because CS glycoforms deposited by purified astrocytes in vitro were removable by digestion with chondroitinase ABC, we attempted to achieve likewise by infusion of the enzyme to the host side of the interface. By 1 month post-treatment, significant numbers of regenerating axons crossed an interface that was subdued in macrophage/microglia reaction and decreased in CS-immunopositivity. The axons extended as far into the caudal cord as 5 mm, in contrast to nil in vehicle-infused controls. Fascicular organizations of axon-Schwann cell units within the regenerated tissue cable were better-preserved in enzyme-treated cords than in vehicle-infused controls. We conclude that CS glycoforms deposited during gliosis at the distal graft-host interface could be cleared by the in vivo action of chondroitinase ABC to improve prospects of axonal regeneration into the host spinal cord.en_HK
dc.languageengen_US
dc.relation.ispartofThe FASEB journal : official publication of the Federation of American Societies for Experimental Biologyen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshAstrocytes - Physiologyen_US
dc.subject.meshAxons - Drug Effects - Physiology - Ultrastructureen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshChondroitin Abc Lyase - Pharmacologyen_US
dc.subject.meshChondroitin Sulfates - Analysis - Immunology - Metabolismen_US
dc.subject.meshMacrophages - Physiologyen_US
dc.subject.meshMyelin Sheath - Physiologyen_US
dc.subject.meshNerve Regeneration - Drug Effectsen_US
dc.subject.meshRatsen_US
dc.subject.meshSchwann Cells - Transplantationen_US
dc.subject.meshSpinal Cord - Cytology - Physiologyen_US
dc.titleChondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury.en_HK
dc.typeArticleen_HK
dc.identifier.emailChau, CH: mchchau@hkucc.hku.hken_HK
dc.identifier.emailShum, DK: shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChau, CH=rp00398en_HK
dc.identifier.authorityShum, DK=rp00321en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1096/fj.03-0196fje-
dc.identifier.pmid14630702-
dc.identifier.scopuseid_2-s2.0-1342268329en_HK
dc.identifier.hkuros93224-
dc.identifier.volume18en_HK
dc.identifier.issue1en_HK
dc.identifier.spage194en_HK
dc.identifier.epage196en_HK
dc.identifier.isiWOS:000188829300071-
dc.identifier.scopusauthoridChau, CH=10040110500en_HK
dc.identifier.scopusauthoridShum, DK=7004824447en_HK
dc.identifier.scopusauthoridLi, H=37062708600en_HK
dc.identifier.scopusauthoridPei, J=7103298575en_HK
dc.identifier.scopusauthoridLui, YY=7004549092en_HK
dc.identifier.scopusauthoridWirthlin, L=36868016600en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridXu, XM=7405298291en_HK

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