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Article: Pseudomonas aeruginosa adherence to human basement membrane collagen in vitro

TitlePseudomonas aeruginosa adherence to human basement membrane collagen in vitro
Authors
KeywordsBacterial adherence
Basement membrane
Collagen
Pseudomonas aeruginosa
Issue Date2003
PublisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
Citation
European Respiratory Journal, 2003, v. 21 n. 6, p. 932-938 How to Cite?
AbstractThe mechanisms for Pseudomonas aeruginosa colonisation in the airways of patients with bronchiectasis and cystic fibrosis are poorly understood. P. aeruginosa could evade mucociliary clearance by adhering to the basement membrane at areas denuded of,intact respiratory epithelium. The authors have developed an in vitro model to study P. aeruginosa adherence to human basement membrane type-IV collagen by using scanning electron microscopy. P. aeruginosa adherence density was determined as the number of P. aeruginosa per 20 microscope fields (2,000x) to log inocular size after incubation at 37°C for 45 min. The presence of phytohaemagglutinin (PHA)-E, which binds specifically to D-galactose-β1-4-D-N-acetylglucosamine, significantly reduced P. aeruginosa adherence density compared with control. The presence of heparin and calcium also significantly reduced P. aeruginosa adherence density. P. aeruginosa adherence was not affected by the presence of proline, trans-hydroxyproline, glycine, galactose, N-acetylneuraminic acid, N-acetylglucosamine or Arachis hypogea. Pseudomonas aeruginosa adherence probably acts via recognition of the D-galactose-β1-4-D-N-acetylglucosamine sequence on type-IV collagen and this process could be inhibited by heparin and calcium. As persistent Pseudomonas aeruginosa colonisation is detrimental to patients with cystic fibrosis and bronchiectasis and there is currently no effective treatment for its eradication, these results could lead to novel therapy for persistent Pseudomonas aeruginosa infection.
Persistent Identifierhttp://hdl.handle.net/10722/147485
ISSN
2015 Impact Factor: 8.332
2015 SCImago Journal Rankings: 3.204
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTsang, KWen_HK
dc.contributor.authorShum, DKen_HK
dc.contributor.authorChan, Sen_HK
dc.contributor.authorNg, Pen_HK
dc.contributor.authorMak, Jen_HK
dc.contributor.authorLeung, Ren_HK
dc.contributor.authorShum, IHen_HK
dc.contributor.authorOoi, GCen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorLam, WKen_HK
dc.date.accessioned2012-05-29T06:04:03Z-
dc.date.available2012-05-29T06:04:03Z-
dc.date.issued2003en_HK
dc.identifier.citationEuropean Respiratory Journal, 2003, v. 21 n. 6, p. 932-938en_HK
dc.identifier.issn0903-1936en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147485-
dc.description.abstractThe mechanisms for Pseudomonas aeruginosa colonisation in the airways of patients with bronchiectasis and cystic fibrosis are poorly understood. P. aeruginosa could evade mucociliary clearance by adhering to the basement membrane at areas denuded of,intact respiratory epithelium. The authors have developed an in vitro model to study P. aeruginosa adherence to human basement membrane type-IV collagen by using scanning electron microscopy. P. aeruginosa adherence density was determined as the number of P. aeruginosa per 20 microscope fields (2,000x) to log inocular size after incubation at 37°C for 45 min. The presence of phytohaemagglutinin (PHA)-E, which binds specifically to D-galactose-β1-4-D-N-acetylglucosamine, significantly reduced P. aeruginosa adherence density compared with control. The presence of heparin and calcium also significantly reduced P. aeruginosa adherence density. P. aeruginosa adherence was not affected by the presence of proline, trans-hydroxyproline, glycine, galactose, N-acetylneuraminic acid, N-acetylglucosamine or Arachis hypogea. Pseudomonas aeruginosa adherence probably acts via recognition of the D-galactose-β1-4-D-N-acetylglucosamine sequence on type-IV collagen and this process could be inhibited by heparin and calcium. As persistent Pseudomonas aeruginosa colonisation is detrimental to patients with cystic fibrosis and bronchiectasis and there is currently no effective treatment for its eradication, these results could lead to novel therapy for persistent Pseudomonas aeruginosa infection.en_HK
dc.languageengen_US
dc.publisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.comen_HK
dc.relation.ispartofEuropean Respiratory Journalen_HK
dc.subjectBacterial adherenceen_HK
dc.subjectBasement membraneen_HK
dc.subjectCollagenen_HK
dc.subjectPseudomonas aeruginosaen_HK
dc.subject.meshAmino Acids - Pharmacologyen_US
dc.subject.meshBacterial Adhesion - Drug Effects - Physiologyen_US
dc.subject.meshBasement Membrane - Drug Effects - Physiopathology - Ultrastructureen_US
dc.subject.meshBronchiectasis - Physiopathologyen_US
dc.subject.meshCarbohydrates - Pharmacologyen_US
dc.subject.meshCollagen Type Iv - Drug Effects - Physiology - Ultrastructureen_US
dc.subject.meshColony Count, Microbialen_US
dc.subject.meshCystic Fibrosis - Physiopathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshIons - Pharmacologyen_US
dc.subject.meshLectins - Pharmacologyen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshPseudomonas Aeruginosa - Drug Effects - Physiology - Ultrastructureen_US
dc.titlePseudomonas aeruginosa adherence to human basement membrane collagen in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailShum, DK:shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailMak, J:judymak@hku.hken_HK
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_HK
dc.identifier.authorityShum, DK=rp00321en_HK
dc.identifier.authorityMak, J=rp00352en_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid12797484-
dc.identifier.scopuseid_2-s2.0-0038652119en_HK
dc.identifier.hkuros79041-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038652119&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue6en_HK
dc.identifier.spage932en_HK
dc.identifier.epage938en_HK
dc.identifier.isiWOS:000183242900005-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridTsang, KW=7201555024en_HK
dc.identifier.scopusauthoridShum, DK=7004824447en_HK
dc.identifier.scopusauthoridChan, S=14055434000en_HK
dc.identifier.scopusauthoridNg, P=55107639000en_HK
dc.identifier.scopusauthoridMak, J=7103323094en_HK
dc.identifier.scopusauthoridLeung, R=7101876102en_HK
dc.identifier.scopusauthoridShum, IH=6602960338en_HK
dc.identifier.scopusauthoridOoi, GC=7006176119en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridLam, WK=7203021937en_HK

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