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Article: A tissue-specific exon of Myosin Va is responsible for selective cargo binding in melanocytes

TitleA tissue-specific exon of Myosin Va is responsible for selective cargo binding in melanocytes
Authors
Issue Date2002
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0886-1544/
Citation
Cell Motility And The Cytoskeleton, 2002, v. 53 n. 2, p. 89-102 How to Cite?
AbstractClass V myosins are molecular motors used for intracellular transportation and organelle tethering. The mouse Myosin Va (MyoVa) is encoded by the dilute locus, which is alternatively spliced to generate several tissue specific isoforms. The tail of MyoVa is the putative cargo-binding domain. To determine the functions of different isoforms of MyoVa and the minimal cargo-binding region, we tagged various isoforms and different portions of the mouse MyoVa tail with a green fluorescent protein and examined their intracellular localizations in the mouse melan-a cells. We found that the amino acid sequence encoded by an alternatively spliced exon, exon F, is necessary for the selective binding of MyoVa to melanosome. The MyoVa isoforms lacking this amino acid sequence are not targeted to the melanosomes, but localized to the perinuclear region instead. These findings suggested that MyoVa is able to bind to more than one types of cargos, with the selectivities determined by alternative spliced sequences. © 2002 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/147474
ISSN
2011 Impact Factor: 4.194
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAu, JSYen_US
dc.contributor.authorHuang, JDen_US
dc.date.accessioned2012-05-29T06:03:58Z-
dc.date.available2012-05-29T06:03:58Z-
dc.date.issued2002en_US
dc.identifier.citationCell Motility And The Cytoskeleton, 2002, v. 53 n. 2, p. 89-102en_US
dc.identifier.issn0886-1544en_US
dc.identifier.urihttp://hdl.handle.net/10722/147474-
dc.description.abstractClass V myosins are molecular motors used for intracellular transportation and organelle tethering. The mouse Myosin Va (MyoVa) is encoded by the dilute locus, which is alternatively spliced to generate several tissue specific isoforms. The tail of MyoVa is the putative cargo-binding domain. To determine the functions of different isoforms of MyoVa and the minimal cargo-binding region, we tagged various isoforms and different portions of the mouse MyoVa tail with a green fluorescent protein and examined their intracellular localizations in the mouse melan-a cells. We found that the amino acid sequence encoded by an alternatively spliced exon, exon F, is necessary for the selective binding of MyoVa to melanosome. The MyoVa isoforms lacking this amino acid sequence are not targeted to the melanosomes, but localized to the perinuclear region instead. These findings suggested that MyoVa is able to bind to more than one types of cargos, with the selectivities determined by alternative spliced sequences. © 2002 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0886-1544/en_US
dc.relation.ispartofCell Motility and the Cytoskeletonen_US
dc.subject.meshAlternative Splicingen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBiological Transport - Physiologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshExons - Geneticsen_US
dc.subject.meshGreen Fluorescent Proteinsen_US
dc.subject.meshLuminescent Proteins - Metabolismen_US
dc.subject.meshMelanocytes - Cytology - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Motor Proteins - Genetics - Metabolismen_US
dc.subject.meshMutationen_US
dc.subject.meshMyosin Heavy Chains - Genetics - Metabolismen_US
dc.subject.meshMyosin Type V - Genetics - Metabolismen_US
dc.subject.meshPc12 Cellsen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshProtein Isoforms - Genetics - Metabolismen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshRatsen_US
dc.subject.meshRecombinant Fusion Proteins - Genetics - Metabolismen_US
dc.titleA tissue-specific exon of Myosin Va is responsible for selective cargo binding in melanocytesen_US
dc.typeArticleen_US
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_US
dc.identifier.authorityHuang, JD=rp00451en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/cm.10061en_US
dc.identifier.pmid12211107en_US
dc.identifier.scopuseid_2-s2.0-0036786015en_US
dc.identifier.hkuros80441-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036786015&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume53en_US
dc.identifier.issue2en_US
dc.identifier.spage89en_US
dc.identifier.epage102en_US
dc.identifier.isiWOS:000178272000002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridAu, JSY=16041329000en_US
dc.identifier.scopusauthoridHuang, JD=8108660600en_US

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