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- Publisher Website: 10.1002/cm.10061
- Scopus: eid_2-s2.0-0036786015
- PMID: 12211107
- WOS: WOS:000178272000002
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Article: A tissue-specific exon of Myosin Va is responsible for selective cargo binding in melanocytes
Title | A tissue-specific exon of Myosin Va is responsible for selective cargo binding in melanocytes |
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Authors | |
Keywords | Green fluorescent protein Melanosome Myosin Myosin-Va |
Issue Date | 2002 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0886-1544/ |
Citation | Cell Motility And The Cytoskeleton, 2002, v. 53 n. 2, p. 89-102 How to Cite? |
Abstract | Class V myosins are molecular motors used for intracellular transportation and organelle tethering. The mouse Myosin Va (MyoVa) is encoded by the dilute locus, which is alternatively spliced to generate several tissue specific isoforms. The tail of MyoVa is the putative cargo-binding domain. To determine the functions of different isoforms of MyoVa and the minimal cargo-binding region, we tagged various isoforms and different portions of the mouse MyoVa tail with a green fluorescent protein and examined their intracellular localizations in the mouse melan-a cells. We found that the amino acid sequence encoded by an alternatively spliced exon, exon F, is necessary for the selective binding of MyoVa to melanosome. The MyoVa isoforms lacking this amino acid sequence are not targeted to the melanosomes, but localized to the perinuclear region instead. These findings suggested that MyoVa is able to bind to more than one types of cargos, with the selectivities determined by alternative spliced sequences. © 2002 Wiley-Liss, Inc. |
Persistent Identifier | http://hdl.handle.net/10722/147474 |
ISSN | 2011 Impact Factor: 4.194 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Au, JSY | en_US |
dc.contributor.author | Huang, JD | en_US |
dc.date.accessioned | 2012-05-29T06:03:58Z | - |
dc.date.available | 2012-05-29T06:03:58Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Cell Motility And The Cytoskeleton, 2002, v. 53 n. 2, p. 89-102 | en_US |
dc.identifier.issn | 0886-1544 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147474 | - |
dc.description.abstract | Class V myosins are molecular motors used for intracellular transportation and organelle tethering. The mouse Myosin Va (MyoVa) is encoded by the dilute locus, which is alternatively spliced to generate several tissue specific isoforms. The tail of MyoVa is the putative cargo-binding domain. To determine the functions of different isoforms of MyoVa and the minimal cargo-binding region, we tagged various isoforms and different portions of the mouse MyoVa tail with a green fluorescent protein and examined their intracellular localizations in the mouse melan-a cells. We found that the amino acid sequence encoded by an alternatively spliced exon, exon F, is necessary for the selective binding of MyoVa to melanosome. The MyoVa isoforms lacking this amino acid sequence are not targeted to the melanosomes, but localized to the perinuclear region instead. These findings suggested that MyoVa is able to bind to more than one types of cargos, with the selectivities determined by alternative spliced sequences. © 2002 Wiley-Liss, Inc. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0886-1544/ | en_US |
dc.relation.ispartof | Cell Motility and the Cytoskeleton | en_US |
dc.subject | Green fluorescent protein | - |
dc.subject | Melanosome | - |
dc.subject | Myosin | - |
dc.subject | Myosin-Va | - |
dc.subject.mesh | Alternative Splicing | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Binding Sites | en_US |
dc.subject.mesh | Biological Transport - Physiology | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Exons - Genetics | en_US |
dc.subject.mesh | Green Fluorescent Proteins | en_US |
dc.subject.mesh | Luminescent Proteins - Metabolism | en_US |
dc.subject.mesh | Melanocytes - Cytology - Metabolism | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Molecular Motor Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Mutation | en_US |
dc.subject.mesh | Myosin Heavy Chains - Genetics - Metabolism | en_US |
dc.subject.mesh | Myosin Type V - Genetics - Metabolism | en_US |
dc.subject.mesh | Pc12 Cells | en_US |
dc.subject.mesh | Phenotype | en_US |
dc.subject.mesh | Protein Binding | en_US |
dc.subject.mesh | Protein Isoforms - Genetics - Metabolism | en_US |
dc.subject.mesh | Protein Structure, Tertiary | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Recombinant Fusion Proteins - Genetics - Metabolism | en_US |
dc.title | A tissue-specific exon of Myosin Va is responsible for selective cargo binding in melanocytes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Huang, JD:jdhuang@hkucc.hku.hk | en_US |
dc.identifier.authority | Huang, JD=rp00451 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/cm.10061 | en_US |
dc.identifier.pmid | 12211107 | en_US |
dc.identifier.scopus | eid_2-s2.0-0036786015 | en_US |
dc.identifier.hkuros | 80441 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036786015&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 53 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 89 | en_US |
dc.identifier.epage | 102 | en_US |
dc.identifier.isi | WOS:000178272000002 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Au, JSY=16041329000 | en_US |
dc.identifier.scopusauthorid | Huang, JD=8108660600 | en_US |
dc.identifier.issnl | 0886-1544 | - |