File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: CARP is a novel caspase recruitment domain containing pro-apoptotic protein

TitleCARP is a novel caspase recruitment domain containing pro-apoptotic protein
Authors
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2002, v. 293 n. 5, p. 1396-1404 How to Cite?
AbstractMany CARD-containing caspase mediators interact with CARD-containing caspases and participate in activation or suppression of caspases. We cloned a novel CARD-containing protein from our EST database, named CARP. Computational characterization revealed that CARP encoded 445 amino acids with predicted MW 49.7 kDa, localized at chromosome 10p13 with 15 exons, and four putative function domains, one CARD domain (aa 160-243), one nuclear receptor-binding motif, two EF-hand motifs, and 42% α-helix content. Stable transfection of CARP into lung carcinoma A549 and HEK293S cells leads to 23% of the cells undergoing apoptosis, but only 3% in the cells transfected with empty control vector. The cell proliferation was significantly inhibited by 1.2-5 folds (P < 0:02) in seven CARP-transfected tumor cell lines-lung carcinoma A549 and PG, melanoma WM451, prostate cancer PC-3 and PC-3M, liver cancer H7402, and bladder cancer BIU87. Our results suggest that CARP is a novel CARD-containing pro-apoptotic protein. © 2002 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/147469
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Ben_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorChen, Jen_US
dc.contributor.authorWei, Zen_US
dc.contributor.authorYu, Hen_US
dc.contributor.authorZhen, Yen_US
dc.contributor.authorLu, Len_US
dc.contributor.authorHui, Ren_US
dc.date.accessioned2012-05-29T06:03:56Z-
dc.date.available2012-05-29T06:03:56Z-
dc.date.issued2002en_US
dc.identifier.citationBiochemical And Biophysical Research Communications, 2002, v. 293 n. 5, p. 1396-1404en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/147469-
dc.description.abstractMany CARD-containing caspase mediators interact with CARD-containing caspases and participate in activation or suppression of caspases. We cloned a novel CARD-containing protein from our EST database, named CARP. Computational characterization revealed that CARP encoded 445 amino acids with predicted MW 49.7 kDa, localized at chromosome 10p13 with 15 exons, and four putative function domains, one CARD domain (aa 160-243), one nuclear receptor-binding motif, two EF-hand motifs, and 42% α-helix content. Stable transfection of CARP into lung carcinoma A549 and HEK293S cells leads to 23% of the cells undergoing apoptosis, but only 3% in the cells transfected with empty control vector. The cell proliferation was significantly inhibited by 1.2-5 folds (P < 0:02) in seven CARP-transfected tumor cell lines-lung carcinoma A549 and PG, melanoma WM451, prostate cancer PC-3 and PC-3M, liver cancer H7402, and bladder cancer BIU87. Our results suggest that CARP is a novel CARD-containing pro-apoptotic protein. © 2002 Elsevier Science (USA). All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.subject.meshAmino Acid Motifsen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna, Complementary - Metabolismen_US
dc.subject.meshDatabases As Topicen_US
dc.subject.meshExpressed Sequence Tagsen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshGreen Fluorescent Proteinsen_US
dc.subject.meshHumansen_US
dc.subject.meshLuminescent Proteins - Metabolismen_US
dc.subject.meshMicroscopy, Fluorescenceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligonucleotides, Antisense - Pharmacologyen_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshPlasmids - Metabolismen_US
dc.subject.meshProtein Kinase C - Metabolismen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshProteins - Chemistry - Physiologyen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRecombinant Fusion Proteins - Metabolismen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshTransfectionen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleCARP is a novel caspase recruitment domain containing pro-apoptotic proteinen_US
dc.typeArticleen_US
dc.identifier.emailLiu, B:ppliew@hkusua.hku.hken_US
dc.identifier.authorityLiu, B=rp01485en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0006-291X(02)00379-0en_US
dc.identifier.pmid12054670en_US
dc.identifier.scopuseid_2-s2.0-0036079260en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036079260&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume293en_US
dc.identifier.issue5en_US
dc.identifier.spage1396en_US
dc.identifier.epage1404en_US
dc.identifier.isiWOS:000176107900016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLiu, B=7408693394en_US
dc.identifier.scopusauthoridLiu, Y=14627533300en_US
dc.identifier.scopusauthoridChen, J=23033557300en_US
dc.identifier.scopusauthoridWei, Z=7402258670en_US
dc.identifier.scopusauthoridYu, H=23981835300en_US
dc.identifier.scopusauthoridZhen, Y=8608594300en_US
dc.identifier.scopusauthoridLu, L=7403963778en_US
dc.identifier.scopusauthoridHui, R=7006029377en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats