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Article: The role of kreisler in segmentation during hindbrain development

TitleThe role of kreisler in segmentation during hindbrain development
Authors
Issue Date1999
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 1999, v. 211 n. 2, p. 220-237 How to Cite?
AbstractThe mouse kreisler gene is expressed in rhombomeres (r) 5 and 6 during neural development and kreisler mutants have patterning defects in the hindbrain that are not fully understood. Here we analyzed this phenotype with a combination of genetic, molecular, and cellular marking techniques. Using Hox/lacZ transgenic mice as reporter lines and by analyzing Eph/ephrin expression, we have found that while r5 fails to form in these mice, r6 is present. This shows that kreisler has an early role in the formation of r5. We also observed patterning defects in r3 and r4 that are outside the normal domain of kreisler expression. In both heterozygous and homozygous kreisler embryos some r5 markers are induced in r3, suggesting that there is a partial change in r3 identity that is not dependent upon the loss of r5. To investigate the cellular character of r6 in kreisler embryos we performed heterotopic grafting experiments in the mouse hindbrain to monitor its mixing properties. Control experiments revealed that cells from even- or odd- numbered segments only mixed freely with themselves, but not with cells of opposite character. Transposition of cells from the r6 territory of kreisler mutants reveals that they adopt mature r6 characteristics, as they freely mix only with cells from even-numbered rhombomeres. Analysis of Phox2b expression shows that some aspects of later neurogenesis in r6 are altered, which may be associated with the additional roles of kreisler in regulating segmental identity. Together these results suggest that the formation of r6 has not been affected in kreisler mutants. This analysis has revealed phenotypic and mechanistic differences between kreisler and its zebrafish equivalent valentino. While valentino is believed to subdivide preexisting segmental units, in the mouse kreisler specifies a particular segment. The formation of r6 independent of r5 argues against a role of kreisler in prorhombomeric segmentation of the mouse hindbrain. We conclude that the mouse kreisler gene regulates multiple steps in segmental patterning involving both the formation of segments and their A-P identity.
Persistent Identifierhttp://hdl.handle.net/10722/147441
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorManzanares, Men_US
dc.contributor.authorTrainor, PAen_US
dc.contributor.authorNonchev, Sen_US
dc.contributor.authorArizaMcnaughton, Len_US
dc.contributor.authorBrodie, Jen_US
dc.contributor.authorGould, Aen_US
dc.contributor.authorMarshall, Hen_US
dc.contributor.authorMorrison, Aen_US
dc.contributor.authorKwan, CTen_US
dc.contributor.authorSham, MHen_US
dc.contributor.authorWilkinson, DGen_US
dc.contributor.authorKrumlauf, Ren_US
dc.date.accessioned2012-05-29T06:03:45Z-
dc.date.available2012-05-29T06:03:45Z-
dc.date.issued1999en_US
dc.identifier.citationDevelopmental Biology, 1999, v. 211 n. 2, p. 220-237en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/10722/147441-
dc.description.abstractThe mouse kreisler gene is expressed in rhombomeres (r) 5 and 6 during neural development and kreisler mutants have patterning defects in the hindbrain that are not fully understood. Here we analyzed this phenotype with a combination of genetic, molecular, and cellular marking techniques. Using Hox/lacZ transgenic mice as reporter lines and by analyzing Eph/ephrin expression, we have found that while r5 fails to form in these mice, r6 is present. This shows that kreisler has an early role in the formation of r5. We also observed patterning defects in r3 and r4 that are outside the normal domain of kreisler expression. In both heterozygous and homozygous kreisler embryos some r5 markers are induced in r3, suggesting that there is a partial change in r3 identity that is not dependent upon the loss of r5. To investigate the cellular character of r6 in kreisler embryos we performed heterotopic grafting experiments in the mouse hindbrain to monitor its mixing properties. Control experiments revealed that cells from even- or odd- numbered segments only mixed freely with themselves, but not with cells of opposite character. Transposition of cells from the r6 territory of kreisler mutants reveals that they adopt mature r6 characteristics, as they freely mix only with cells from even-numbered rhombomeres. Analysis of Phox2b expression shows that some aspects of later neurogenesis in r6 are altered, which may be associated with the additional roles of kreisler in regulating segmental identity. Together these results suggest that the formation of r6 has not been affected in kreisler mutants. This analysis has revealed phenotypic and mechanistic differences between kreisler and its zebrafish equivalent valentino. While valentino is believed to subdivide preexisting segmental units, in the mouse kreisler specifies a particular segment. The formation of r6 independent of r5 argues against a role of kreisler in prorhombomeric segmentation of the mouse hindbrain. We conclude that the mouse kreisler gene regulates multiple steps in segmental patterning involving both the formation of segments and their A-P identity.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_US
dc.relation.ispartofDevelopmental Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAvian Proteinsen_US
dc.subject.meshDna-Binding Proteins - Physiologyen_US
dc.subject.meshEmbryonic And Fetal Development - Physiologyen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshLeucine Zippers - Physiologyen_US
dc.subject.meshMaf Transcription Factorsen_US
dc.subject.meshMafb Transcription Factoren_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMice, Inbred Cbaen_US
dc.subject.meshOncogene Proteinsen_US
dc.subject.meshRhombencephalon - Embryology - Physiologyen_US
dc.subject.meshTranscription Factors - Physiologyen_US
dc.subject.meshZebrafish Proteinsen_US
dc.titleThe role of kreisler in segmentation during hindbrain developmenten_US
dc.typeArticleen_US
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_US
dc.identifier.authoritySham, MH=rp00380en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/dbio.1999.9318en_US
dc.identifier.pmid10395784-
dc.identifier.scopuseid_2-s2.0-0033566053en_US
dc.identifier.hkuros43427-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033566053&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume211en_US
dc.identifier.issue2en_US
dc.identifier.spage220en_US
dc.identifier.epage237en_US
dc.identifier.isiWOS:000081625000005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridManzanares, M=7003611983en_US
dc.identifier.scopusauthoridTrainor, PA=7003894254en_US
dc.identifier.scopusauthoridNonchev, S=6603818489en_US
dc.identifier.scopusauthoridArizaMcNaughton, L=6601980638en_US
dc.identifier.scopusauthoridBrodie, J=7102849428en_US
dc.identifier.scopusauthoridGould, A=7201576107en_US
dc.identifier.scopusauthoridMarshall, H=7102515675en_US
dc.identifier.scopusauthoridMorrison, A=7402256918en_US
dc.identifier.scopusauthoridKwan, CT=7201421142en_US
dc.identifier.scopusauthoridSham, MH=7003729109en_US
dc.identifier.scopusauthoridWilkinson, DG=7401870302en_US
dc.identifier.scopusauthoridKrumlauf, R=7006242495en_US

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