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Article: The role of negative superhelicity and length of homology in the formation of paranemic joints promoted by RecA protein

TitleThe role of negative superhelicity and length of homology in the formation of paranemic joints promoted by RecA protein
Authors
Issue Date1998
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1998, v. 273 n. 20, p. 12120-12127 How to Cite?
AbstractEscherichia coli RecA protein pairs homologous DNA molecules to form paranemic joints when there is an absence of a free end in the region of homologous contact. Paranemic joints are a key intermediate in homologous recombination and are important in understanding the mechanism for a search of hemology. The efficiency of paranemic joint formation depended on the length of homology and the topological forms of the duplex DNA. The presence of negative superhelicity increased the pairing efficiency and reduced the minimal length of homology required for paranemic joint formation. Negative superhelicity stimulated joint formation by favoring the initial unwinding of duplex DNA that occurred during the homology search and was not essential in the maintenance of the paired structure. Regardless of length of homology, formation of paranemic joints using circular duplex DNA required the presence of more than six negative supercoils. Above six negative turns, an increasing degree of negative superhelicity resulted in a linear increase in the pairing efficiency. These results support a model of two distinct kinds of DNA unwinding occurring in paranemic joint formation: an initial unwinding caused by heterologous contacts during synapsis and a later one during pairing of the homologous molecules.
Persistent Identifierhttp://hdl.handle.net/10722/147434
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, BCen_US
dc.contributor.authorChiu, SKen_US
dc.contributor.authorChow, SAen_US
dc.date.accessioned2012-05-29T06:03:42Z-
dc.date.available2012-05-29T06:03:42Z-
dc.date.issued1998en_US
dc.identifier.citationJournal Of Biological Chemistry, 1998, v. 273 n. 20, p. 12120-12127en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/147434-
dc.description.abstractEscherichia coli RecA protein pairs homologous DNA molecules to form paranemic joints when there is an absence of a free end in the region of homologous contact. Paranemic joints are a key intermediate in homologous recombination and are important in understanding the mechanism for a search of hemology. The efficiency of paranemic joint formation depended on the length of homology and the topological forms of the duplex DNA. The presence of negative superhelicity increased the pairing efficiency and reduced the minimal length of homology required for paranemic joint formation. Negative superhelicity stimulated joint formation by favoring the initial unwinding of duplex DNA that occurred during the homology search and was not essential in the maintenance of the paired structure. Regardless of length of homology, formation of paranemic joints using circular duplex DNA required the presence of more than six negative supercoils. Above six negative turns, an increasing degree of negative superhelicity resulted in a linear increase in the pairing efficiency. These results support a model of two distinct kinds of DNA unwinding occurring in paranemic joint formation: an initial unwinding caused by heterologous contacts during synapsis and a later one during pairing of the homologous molecules.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshBacteriophage Phi X 174 - Geneticsen_US
dc.subject.meshDna, Bacterial - Genetics - Metabolismen_US
dc.subject.meshDna, Circular - Genetics - Metabolismen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshEscherichia Coli - Geneticsen_US
dc.subject.meshMicrovirus - Geneticsen_US
dc.subject.meshRec A Recombinases - Metabolismen_US
dc.subject.meshRecombination, Geneticen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.titleThe role of negative superhelicity and length of homology in the formation of paranemic joints promoted by RecA proteinen_US
dc.typeArticleen_US
dc.identifier.emailWong, BC:bcwwong@hkucc.hku.hken_US
dc.identifier.authorityWong, BC=rp00369en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.273.20.12120en_US
dc.identifier.pmid9575157-
dc.identifier.scopuseid_2-s2.0-0032524286en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032524286&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume273en_US
dc.identifier.issue20en_US
dc.identifier.spage12120en_US
dc.identifier.epage12127en_US
dc.identifier.isiWOS:000073629800022-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, BC=35733052400en_US
dc.identifier.scopusauthoridChiu, SK=7202291671en_US
dc.identifier.scopusauthoridChow, SA=7201827867en_US

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