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Article: In vitro expression analysis of collagen biosynthesis and assembly

TitleIn vitro expression analysis of collagen biosynthesis and assembly
Authors
Issue Date1997
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbbm
Citation
Journal Of Biochemical And Biophysical Methods, 1997, v. 36 n. 1, p. 11-29 How to Cite?
AbstractWhile the generalised pathway of collagen biosynthesis is well understood, the specific molecular interactions that drive chain recognition and assembly and the formation of tissue-specific extracellular supramolecular structures have not been elucidated. This review focuses on the use of in vitro collagen expression systems to explore some of these fundamental questions on the molecular basis of normal and mutant collagen assembly. Three in vitro expression/assembly systems are discussed. Firstly, a simple cell-free transcription/translation system to study the initial stages of collagen chain assembly. Secondly, a novel T7-driven high level expression system, using a recombinant vaccinia virus expressing T7 RNA polymerase, in transiently transfected cells which allows appropriate postranslational modification and collagen folding. Thirdly, the more complex questions of normal and mutant collagen extracellular matrix assembly are addressed by stable transfection and expression in cells which allow the formation of a 'tissue equivalent' matrix during long-term culture.
Persistent Identifierhttp://hdl.handle.net/10722/147423
ISSN
2010 Impact Factor: 1.808
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, Den_US
dc.contributor.authorLamandé, SRen_US
dc.contributor.authorMcquillan, DJen_US
dc.contributor.authorBateman, JFen_US
dc.date.accessioned2012-05-29T06:03:37Z-
dc.date.available2012-05-29T06:03:37Z-
dc.date.issued1997en_US
dc.identifier.citationJournal Of Biochemical And Biophysical Methods, 1997, v. 36 n. 1, p. 11-29en_US
dc.identifier.issn0165-022Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/147423-
dc.description.abstractWhile the generalised pathway of collagen biosynthesis is well understood, the specific molecular interactions that drive chain recognition and assembly and the formation of tissue-specific extracellular supramolecular structures have not been elucidated. This review focuses on the use of in vitro collagen expression systems to explore some of these fundamental questions on the molecular basis of normal and mutant collagen assembly. Three in vitro expression/assembly systems are discussed. Firstly, a simple cell-free transcription/translation system to study the initial stages of collagen chain assembly. Secondly, a novel T7-driven high level expression system, using a recombinant vaccinia virus expressing T7 RNA polymerase, in transiently transfected cells which allows appropriate postranslational modification and collagen folding. Thirdly, the more complex questions of normal and mutant collagen extracellular matrix assembly are addressed by stable transfection and expression in cells which allow the formation of a 'tissue equivalent' matrix during long-term culture.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbbmen_US
dc.relation.ispartofJournal of Biochemical and Biophysical Methodsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCollagen - Biosynthesis - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMammalsen_US
dc.subject.meshMutationen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRecombinant Proteins - Biosynthesisen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTransfection - Methodsen_US
dc.titleIn vitro expression analysis of collagen biosynthesis and assemblyen_US
dc.typeArticleen_US
dc.identifier.emailChan, D:chand@hkucc.hku.hken_US
dc.identifier.authorityChan, D=rp00540en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0165-022X(97)00042-0en_US
dc.identifier.pmid9507370en_US
dc.identifier.scopuseid_2-s2.0-0031409866en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031409866&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume36en_US
dc.identifier.issue1en_US
dc.identifier.spage11en_US
dc.identifier.epage29en_US
dc.identifier.isiWOS:000071967500003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChan, D=7402216545en_US
dc.identifier.scopusauthoridLamandé, SR=7004500719en_US
dc.identifier.scopusauthoridMcQuillan, DJ=7003319485en_US
dc.identifier.scopusauthoridBateman, JF=16135557700en_US

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