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Article: SOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouse

TitleSOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouse
Authors
Issue Date1997
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 1997, v. 183 n. 1, p. 108-121 How to Cite?
AbstractTwo lines of evidence suggest that the Sry-related gene Sox9 is important for chondrogenesis in mammalian embryos. Sox9 mRNA is expressed in chondrogenic condensations in mice, and mutations in human SOX9 are known to cause skeletal dysplasia. We show here that mouse SOX9 protein is able to bind to a SOX/SRY consensus motif in DNA and contains a modular transcriptional activation domain, consistent with a role for SOX9 as a transcription factor acting on genes involved in cartilage development. One such gene is Col2a1, which encodes type II collagen, the major structural component of cartilage. We have compared, in detail, the expression of Sox9 and Col2a1 during mouse development. In chondrogenic tissues the expression profiles of the two genes were remarkably similar. Coexpression was detected in some nonchondrogenic tissues such as the notochord, otic vesicle, and neural tube, but others such as heart and lung differed in their expression of the two genes. Immunohistochemistry using an antibody specific for SOX9 revealed that expression of SOX9 protein mirrored the distribution of Sox9 mRNA. Our results suggest that SOX9 protein is involved in the regulation of Col2a1 during chondrogenesis, but that this regulation is likely to depend on additional cofactors.
Persistent Identifierhttp://hdl.handle.net/10722/147421
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, LJen_US
dc.contributor.authorWheatley, Sen_US
dc.contributor.authorMuscat, GEOen_US
dc.contributor.authorConwayCampbell, Jen_US
dc.contributor.authorBowles, Jen_US
dc.contributor.authorWright, Een_US
dc.contributor.authorBell, DMen_US
dc.contributor.authorTam, PPLen_US
dc.contributor.authorCheah, KSEen_US
dc.contributor.authorKoopman, Pen_US
dc.date.accessioned2012-05-29T06:03:36Z-
dc.date.available2012-05-29T06:03:36Z-
dc.date.issued1997en_US
dc.identifier.citationDevelopmental Biology, 1997, v. 183 n. 1, p. 108-121en_US
dc.identifier.issn0012-1606en_US
dc.identifier.urihttp://hdl.handle.net/10722/147421-
dc.description.abstractTwo lines of evidence suggest that the Sry-related gene Sox9 is important for chondrogenesis in mammalian embryos. Sox9 mRNA is expressed in chondrogenic condensations in mice, and mutations in human SOX9 are known to cause skeletal dysplasia. We show here that mouse SOX9 protein is able to bind to a SOX/SRY consensus motif in DNA and contains a modular transcriptional activation domain, consistent with a role for SOX9 as a transcription factor acting on genes involved in cartilage development. One such gene is Col2a1, which encodes type II collagen, the major structural component of cartilage. We have compared, in detail, the expression of Sox9 and Col2a1 during mouse development. In chondrogenic tissues the expression profiles of the two genes were remarkably similar. Coexpression was detected in some nonchondrogenic tissues such as the notochord, otic vesicle, and neural tube, but others such as heart and lung differed in their expression of the two genes. Immunohistochemistry using an antibody specific for SOX9 revealed that expression of SOX9 protein mirrored the distribution of Sox9 mRNA. Our results suggest that SOX9 protein is involved in the regulation of Col2a1 during chondrogenesis, but that this regulation is likely to depend on additional cofactors.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_US
dc.relation.ispartofDevelopmental Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCos Cellsen_US
dc.subject.meshCartilage - Embryologyen_US
dc.subject.meshCollagen - Geneticsen_US
dc.subject.meshConsensus Sequence - Geneticsen_US
dc.subject.meshDna - Metabolismen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshEmbryonic And Fetal Developmenten_US
dc.subject.meshGene Expression Regulation, Developmental - Physiologyen_US
dc.subject.meshGerm Layers - Chemistryen_US
dc.subject.meshHigh Mobility Group Proteins - Analysis - Genetics - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshNuclear Proteinsen_US
dc.subject.meshOrgan Specificityen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshSox9 Transcription Factoren_US
dc.subject.meshSequence Deletionen_US
dc.subject.meshSex-Determining Region Y Proteinen_US
dc.subject.meshTranscription Factors - Analysis - Genetics - Metabolismen_US
dc.subject.meshTranscriptional Activation - Physiologyen_US
dc.subject.meshTransfectionen_US
dc.titleSOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouseen_US
dc.typeArticleen_US
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_US
dc.identifier.authorityCheah, KSE=rp00342en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/dbio.1996.8487en_US
dc.identifier.pmid9119111-
dc.identifier.scopuseid_2-s2.0-0031104994en_US
dc.identifier.hkuros25352-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031104994&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume183en_US
dc.identifier.issue1en_US
dc.identifier.spage108en_US
dc.identifier.epage121en_US
dc.identifier.isiWOS:A1997WN27300010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridNg, LJ=7201477760en_US
dc.identifier.scopusauthoridWheatley, S=36959255000en_US
dc.identifier.scopusauthoridMuscat, GEO=7006205187en_US
dc.identifier.scopusauthoridConwayCampbell, J=6504384650en_US
dc.identifier.scopusauthoridBowles, J=7101909227en_US
dc.identifier.scopusauthoridWright, E=7401796136en_US
dc.identifier.scopusauthoridBell, DM=7403648027en_US
dc.identifier.scopusauthoridTam, PPL=7202539412en_US
dc.identifier.scopusauthoridCheah, KSE=35387746200en_US
dc.identifier.scopusauthoridKoopman, P=7102712085en_US
dc.identifier.citeulike8898250-

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