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Article: Analysis of glycosaminoglycans induced in newly formed calcium oxalate crystals using an undiluted urine system

TitleAnalysis of glycosaminoglycans induced in newly formed calcium oxalate crystals using an undiluted urine system
Authors
Issue Date1995
Citation
Scanning Microscopy, 1995, v. 9 n. 4, p. 1089-1096 How to Cite?
AbstractThe aim of this study was to examine the effect of sodium pentosan polysulfate (SPP) in an undiluted urine system and to study its relative affinity to calcium oxalate (CaOx) crystals in the presence or absence of heparan sulfate (HS). CaOx crystals were induced with an overload of oxalate above the metastable limit in spun and filtered urine (SF) and ultrafiltered urine (UF). Then, the crystals were dissolved with EDTA (ethylenediaminetetraacetic acid), electrodialysed and lyophilized. The polyanions, HS or SPP were added to the UF prior to the addition of oxalate. Polyanions in crystal matrices were examined by cellulose acetate electrophoresis. Crystal volume and size were suppressed according to the increase of the concentration of SPP when compared with those of the UF. Scanning electron microscopy (SEM) showed marked aggregation of the crystals in the UF and no aggregation in the presence of SPP. HS was the only polyanion found in CaOx crystals formed after overload of oxalate in SF. Crystals formed in UF did not contain any polyanions. When SPP was added to UF, SPP appeared in the crystal matrix in accordance with its concentration. Once HS in physiological concentration was added to the UF containing SPP, HS and SPP obtained from crystals were strongly stained with Alcian blue in electrophoretic study, where SPP is stained stronger than HS. These results suggest that SPP strongly binds to CaOx crystals as well as HS and that HS and SPP competitively bind to the crystal, then, as a result, they are incorporated into the crystals. The fact that SPP suppressed the aggregation of CaOx crystals in undiluted urine showed the possibility that SPP might be one of the useful drugs for preventing CaOx urolithiasis.
Persistent Identifierhttp://hdl.handle.net/10722/147400
ISSN
1999 SCImago Journal Rankings: 0.280
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSuzuki, Ken_US
dc.contributor.authorTsugawa, Ren_US
dc.contributor.authorKok, Den_US
dc.contributor.authorKhan, SRen_US
dc.contributor.authorCao, LCen_US
dc.contributor.authorBoeve, ERen_US
dc.contributor.authorGohel, Ien_US
dc.contributor.authorShum, DKYen_US
dc.contributor.authorBinette, Pen_US
dc.contributor.authorNorman, RWen_US
dc.date.accessioned2012-05-29T06:03:27Z-
dc.date.available2012-05-29T06:03:27Z-
dc.date.issued1995en_US
dc.identifier.citationScanning Microscopy, 1995, v. 9 n. 4, p. 1089-1096en_US
dc.identifier.issn0891-7035en_US
dc.identifier.urihttp://hdl.handle.net/10722/147400-
dc.description.abstractThe aim of this study was to examine the effect of sodium pentosan polysulfate (SPP) in an undiluted urine system and to study its relative affinity to calcium oxalate (CaOx) crystals in the presence or absence of heparan sulfate (HS). CaOx crystals were induced with an overload of oxalate above the metastable limit in spun and filtered urine (SF) and ultrafiltered urine (UF). Then, the crystals were dissolved with EDTA (ethylenediaminetetraacetic acid), electrodialysed and lyophilized. The polyanions, HS or SPP were added to the UF prior to the addition of oxalate. Polyanions in crystal matrices were examined by cellulose acetate electrophoresis. Crystal volume and size were suppressed according to the increase of the concentration of SPP when compared with those of the UF. Scanning electron microscopy (SEM) showed marked aggregation of the crystals in the UF and no aggregation in the presence of SPP. HS was the only polyanion found in CaOx crystals formed after overload of oxalate in SF. Crystals formed in UF did not contain any polyanions. When SPP was added to UF, SPP appeared in the crystal matrix in accordance with its concentration. Once HS in physiological concentration was added to the UF containing SPP, HS and SPP obtained from crystals were strongly stained with Alcian blue in electrophoretic study, where SPP is stained stronger than HS. These results suggest that SPP strongly binds to CaOx crystals as well as HS and that HS and SPP competitively bind to the crystal, then, as a result, they are incorporated into the crystals. The fact that SPP suppressed the aggregation of CaOx crystals in undiluted urine showed the possibility that SPP might be one of the useful drugs for preventing CaOx urolithiasis.en_US
dc.languageengen_US
dc.relation.ispartofScanning Microscopyen_US
dc.subject.meshAdulten_US
dc.subject.meshCalcium Oxalate - Chemistryen_US
dc.subject.meshCrystallizationen_US
dc.subject.meshHeparitin Sulfate - Pharmacology - Urineen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshPentosan Sulfuric Polyester - Pharmacology - Urineen_US
dc.subject.meshUrinary Calculi - Prevention & Controlen_US
dc.titleAnalysis of glycosaminoglycans induced in newly formed calcium oxalate crystals using an undiluted urine systemen_US
dc.typeArticleen_US
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8819890-
dc.identifier.scopuseid_2-s2.0-0029555140en_US
dc.identifier.volume9en_US
dc.identifier.issue4en_US
dc.identifier.spage1089en_US
dc.identifier.epage1096en_US
dc.identifier.isiWOS:A1995TQ87000014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSuzuki, K=7501778300en_US
dc.identifier.scopusauthoridTsugawa, R=7007183714en_US
dc.identifier.scopusauthoridKok, D=8945098900en_US
dc.identifier.scopusauthoridKhan, SR=7404043556en_US
dc.identifier.scopusauthoridCao, LC=7401638173en_US
dc.identifier.scopusauthoridBoeve, ER=7004154014en_US
dc.identifier.scopusauthoridGohel, I=8140002400en_US
dc.identifier.scopusauthoridShum, DKY=7004824447en_US
dc.identifier.scopusauthoridBinette, P=8140002600en_US
dc.identifier.scopusauthoridNorman, RW=7202169794en_US

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