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Article: Electrophoretic modification of sperm plasma membrane by ventral prostate secretion in golden hamsters

TitleElectrophoretic modification of sperm plasma membrane by ventral prostate secretion in golden hamsters
Authors
Issue Date1995
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01485016.asp
Citation
Archives Of Andrology, 1995, v. 35 n. 1, p. 13-20 How to Cite?
AbstractPlasma membrane proteins were extracted either from epididymal spermatozoa after incubation with ventral prostrate gland secretion or from spermatozoa recovered from uteri of females mated with surgically treated males belonging to the following groups: TX (excision of all accessory sex glands, ASG), VPX (bilateral excision of ventral prostate), VP (bilateral excision of all ASG except the ventral prostate), and SH (sham-operated). Incubation of spermatozoa with ventral prostatic secretion resulted in an 11-fold increase in glycoprotein content of the plasma membrane, but total protein concentration remained unchanged. The in vivo study indicated that interactions of ASG secretions and spermatozoa were complicated by the presence of uterine secretions. Glycoprotein content was reduced in the presence of ventral prostatic secretions. SDS-PAGE profiles showed that both uterine and ASG secretions could modify proteins on the sperm surface. Enrichment of a 25-kD subunit was apparently effected by uterine secretions and further promoted by combined secretions of the ampullary gland, coagulating gland, dorsolateral prostate, and seminal vesicle, but was reduced by the ventral prostate. A number of other protein subunits appeared to be specifically modified by the ventral prostate, while other ASG secretions were also shown to alter the effects of the ventral prostate on the sperm surface.
Persistent Identifierhttp://hdl.handle.net/10722/147399
ISSN
2009 Impact Factor: 0.891
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheng, LYLen_US
dc.contributor.authorYuen, ACYen_US
dc.contributor.authorChow, PHen_US
dc.date.accessioned2012-05-29T06:03:26Z-
dc.date.available2012-05-29T06:03:26Z-
dc.date.issued1995en_US
dc.identifier.citationArchives Of Andrology, 1995, v. 35 n. 1, p. 13-20en_US
dc.identifier.issn0148-5016en_US
dc.identifier.urihttp://hdl.handle.net/10722/147399-
dc.description.abstractPlasma membrane proteins were extracted either from epididymal spermatozoa after incubation with ventral prostrate gland secretion or from spermatozoa recovered from uteri of females mated with surgically treated males belonging to the following groups: TX (excision of all accessory sex glands, ASG), VPX (bilateral excision of ventral prostate), VP (bilateral excision of all ASG except the ventral prostate), and SH (sham-operated). Incubation of spermatozoa with ventral prostatic secretion resulted in an 11-fold increase in glycoprotein content of the plasma membrane, but total protein concentration remained unchanged. The in vivo study indicated that interactions of ASG secretions and spermatozoa were complicated by the presence of uterine secretions. Glycoprotein content was reduced in the presence of ventral prostatic secretions. SDS-PAGE profiles showed that both uterine and ASG secretions could modify proteins on the sperm surface. Enrichment of a 25-kD subunit was apparently effected by uterine secretions and further promoted by combined secretions of the ampullary gland, coagulating gland, dorsolateral prostate, and seminal vesicle, but was reduced by the ventral prostate. A number of other protein subunits appeared to be specifically modified by the ventral prostate, while other ASG secretions were also shown to alter the effects of the ventral prostate on the sperm surface.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01485016.aspen_US
dc.relation.ispartofArchives of Andrologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Membrane - Chemistryen_US
dc.subject.meshCopulationen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshExudates And Transudatesen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenitalia, Male - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Glycoproteins - Isolation & Purification - Physiologyen_US
dc.subject.meshMesocricetusen_US
dc.subject.meshProstate - Secretionen_US
dc.subject.meshProstatectomyen_US
dc.subject.meshProteins - Isolation & Purification - Physiologyen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshSpermatozoa - Chemistry - Physiologyen_US
dc.subject.meshUterus - Secretionen_US
dc.titleElectrophoretic modification of sperm plasma membrane by ventral prostate secretion in golden hamstersen_US
dc.typeArticleen_US
dc.identifier.emailCheng, LYL:lcheng@hkucc.hku.hken_US
dc.identifier.authorityCheng, LYL=rp00317en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8554426-
dc.identifier.scopuseid_2-s2.0-0029092783en_US
dc.identifier.volume35en_US
dc.identifier.issue1en_US
dc.identifier.spage13en_US
dc.identifier.epage20en_US
dc.identifier.isiWOS:A1995RJ18400003-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridCheng, LYL=7403337122en_US
dc.identifier.scopusauthoridYuen, ACY=7006290143en_US
dc.identifier.scopusauthoridChow, PH=7202656919en_US

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