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Article: Tissue-specific and differential expression of alternatively spliced α1(II) collagen mRNAs in early human embryos

TitleTissue-specific and differential expression of alternatively spliced α1(II) collagen mRNAs in early human embryos
Authors
KeywordsAlternative splicing
Human α1(II) collagen mRNA
Non-chondrogenic expression
Issue Date1995
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417
Citation
Developmental Dynamics, 1995, v. 203 n. 2, p. 198-211 How to Cite?
AbstractExpression of the α1(II) procollagen gene is not confined to chondrogenic tissues during vertebrate development. Transcripts of the human gene (COL2A1) are alternatively spliced to give mRNAs which either exclude (type IIB mRNA) or include (type IIA mRNA) an exon encoding a cysteine-rich domain in the amino-propeptide. The distribution of COL2A1 mRNAs in 27-to 44-day human embryos and 8- to 24-week fetuses was studied by in situ hybridization and RNase protection analyses. Type HA mRNAs were expressed in prechondrogenic cells and were also preferentially expressed in chondrogenic tissues at regions of chondrocyte commitment and cartilage growth. During maturation of chondrocytes, there is a switch to expression of type IIB mRNAs. In non- chondrogenic tissues of early embryos, type IIA mRNA expression was associated with active tissue remodeling, epithelial organization, and sites of tissue interaction. Type IIA mRNAs were also expressed in some non- chondrogenic tissues where expression had previously been undetected, such as the tooth bud, liver, adrenal cortex, apical ectodermal ridge, and indifferent gonad. In older fetuses type IIA mRNAs were the sole or major transcript in most non-chondrogenic tissues except the choroid plexus and tendon. In the meninges there was a unique switch from type IIB to type IIA expression. The expression pattern of COL2A1 transcripts suggests that, in addition to contributing to the structural integrity of the cartilage extracellular matrix, type II procollagen may serve a morphogenetic role in embryonic development. Our findings clearly show that the pattern of expression of type II procollagen mRNAs is largely conserved between man and mouse. However, some differences exist, and these should be taken into consideration when animal models are used to study human diseases associated with COL2A1.
Persistent Identifierhttp://hdl.handle.net/10722/147398
ISSN
2015 Impact Factor: 2.198
2015 SCImago Journal Rankings: 1.726
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLui, VCHen_HK
dc.contributor.authorNg, LJen_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorTam, PPLen_HK
dc.contributor.authorCheah, KSEen_HK
dc.date.accessioned2012-05-29T06:03:26Z-
dc.date.available2012-05-29T06:03:26Z-
dc.date.issued1995en_HK
dc.identifier.citationDevelopmental Dynamics, 1995, v. 203 n. 2, p. 198-211en_HK
dc.identifier.issn1058-8388en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147398-
dc.description.abstractExpression of the α1(II) procollagen gene is not confined to chondrogenic tissues during vertebrate development. Transcripts of the human gene (COL2A1) are alternatively spliced to give mRNAs which either exclude (type IIB mRNA) or include (type IIA mRNA) an exon encoding a cysteine-rich domain in the amino-propeptide. The distribution of COL2A1 mRNAs in 27-to 44-day human embryos and 8- to 24-week fetuses was studied by in situ hybridization and RNase protection analyses. Type HA mRNAs were expressed in prechondrogenic cells and were also preferentially expressed in chondrogenic tissues at regions of chondrocyte commitment and cartilage growth. During maturation of chondrocytes, there is a switch to expression of type IIB mRNAs. In non- chondrogenic tissues of early embryos, type IIA mRNA expression was associated with active tissue remodeling, epithelial organization, and sites of tissue interaction. Type IIA mRNAs were also expressed in some non- chondrogenic tissues where expression had previously been undetected, such as the tooth bud, liver, adrenal cortex, apical ectodermal ridge, and indifferent gonad. In older fetuses type IIA mRNAs were the sole or major transcript in most non-chondrogenic tissues except the choroid plexus and tendon. In the meninges there was a unique switch from type IIB to type IIA expression. The expression pattern of COL2A1 transcripts suggests that, in addition to contributing to the structural integrity of the cartilage extracellular matrix, type II procollagen may serve a morphogenetic role in embryonic development. Our findings clearly show that the pattern of expression of type II procollagen mRNAs is largely conserved between man and mouse. However, some differences exist, and these should be taken into consideration when animal models are used to study human diseases associated with COL2A1.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417en_HK
dc.relation.ispartofDevelopmental Dynamicsen_HK
dc.rightsDevelopmental Dynamics. Copyright © John Wiley & Sons, Inc.-
dc.subjectAlternative splicingen_HK
dc.subjectHuman α1(II) collagen mRNAen_HK
dc.subjectNon-chondrogenic expressionen_HK
dc.subject.meshAlternative Splicingen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBone And Bones - Embryologyen_US
dc.subject.meshCartilage - Embryologyen_US
dc.subject.meshCollagen - Geneticsen_US
dc.subject.meshEmbryo, Mammalian - Metabolismen_US
dc.subject.meshEmbryonic And Fetal Developmenten_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligonucleotide Probes - Geneticsen_US
dc.subject.meshPregnancyen_US
dc.subject.meshPregnancy Trimester, Firsten_US
dc.subject.meshProcollagen - Geneticsen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshTissue Distributionen_US
dc.titleTissue-specific and differential expression of alternatively spliced α1(II) collagen mRNAs in early human embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.emailNicholls, J: nicholls@pathology.hku.hken_HK
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hken_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid7655082-
dc.identifier.scopuseid_2-s2.0-0029078901en_HK
dc.identifier.hkuros1722-
dc.identifier.hkuros6266-
dc.identifier.volume203en_HK
dc.identifier.issue2en_HK
dc.identifier.spage198en_HK
dc.identifier.epage211en_HK
dc.identifier.isiWOS:A1995RD00900007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK
dc.identifier.scopusauthoridLing Jim Ng=7409711856en_HK
dc.identifier.scopusauthoridNicholls, J=7201463077en_HK
dc.identifier.scopusauthoridTam, PPL=7202539412en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK
dc.customcontrol.immutablesml 130621-

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