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Article: Influence of digits, ectoderm, and retinoic acid on chondrogenesis by mouse interdigital mesoderm in culture

TitleInfluence of digits, ectoderm, and retinoic acid on chondrogenesis by mouse interdigital mesoderm in culture
Authors
Issue Date1994
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417
Citation
Developmental Dynamics, 1994, v. 201 n. 4, p. 297-309 How to Cite?
AbstractWe have cultured tissues isolated from the interdigital zones (IDZ) of the mouse footplate in the presence of the digits, ectoderm, and all-trans retinoic acid. The objective was to understand how these various factors influence the developmental fate of the interdigital tissue. Neutral red staining showed that these tissues normally differentiate by dying between day 12.5-14.5. However, if they were isolated from the footplate between day 12.5-13.5 (when cell death is not overtly obvious in the IDZ) and maintained in organ culture, these tissues would develop into cartilage and soft connective tissues. In culture, chondrogenesis is initiated very rapidly in the interdigital explants as revealed by in situ hybridization with riboprobes specific for type IIA and IIB procollagen mRNAs. The ability of interdigital tissues to form cartilage is not attributed to factors present in the serum of the culture medium as this phenomenon is also observed in serumless cultures. We have found that if all-trans retinoic acid, at concentrations of 10-50 ng/ml culture medium, were added to the explants it could inhibit chondrogenesis and promote cell death. Moreover, in some of the cultures, a single digit was left attached to the interdigital tissue. This also dramatically reduced the incidence of chondrogenesis. We have tried the determine whether the digits and ectoderm can produce a diffusible factor that can prevent cartilage from developing by culturing day 12.5 interdigital tissues in ectoderm and digit conditioned media. The ectoderm conditioned medium had no effects on interdigital growth or chondrogenesis. In contrast, the size of interdigital explants cultured in the presence of digit conditioned medium was shown to be significantly smaller than the control. These explants also produced a smaller quantity of cartilage as revealed by Alcian blue binding assay. In sum, our results showed that the fate of the interdigital tissues are not fully determined until after day 13.5. These tissues have the potentials to form cartilage and soft connective tissues. We tentatively propose that these interdigital tissues do not normally realize their histogenetic potentials because of the antichondrogenic influence of the digits and retinoic acid.
Persistent Identifierhttp://hdl.handle.net/10722/147390
ISSN
2015 Impact Factor: 2.198
2015 SCImago Journal Rankings: 1.726
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, KKHen_US
dc.contributor.authorLi, FCHen_US
dc.contributor.authorYung, WTen_US
dc.contributor.authorKung, JLSen_US
dc.contributor.authorNg, JLen_US
dc.contributor.authorCheah, KSEen_US
dc.date.accessioned2012-05-29T06:03:22Z-
dc.date.available2012-05-29T06:03:22Z-
dc.date.issued1994en_US
dc.identifier.citationDevelopmental Dynamics, 1994, v. 201 n. 4, p. 297-309en_US
dc.identifier.issn1058-8388en_US
dc.identifier.urihttp://hdl.handle.net/10722/147390-
dc.description.abstractWe have cultured tissues isolated from the interdigital zones (IDZ) of the mouse footplate in the presence of the digits, ectoderm, and all-trans retinoic acid. The objective was to understand how these various factors influence the developmental fate of the interdigital tissue. Neutral red staining showed that these tissues normally differentiate by dying between day 12.5-14.5. However, if they were isolated from the footplate between day 12.5-13.5 (when cell death is not overtly obvious in the IDZ) and maintained in organ culture, these tissues would develop into cartilage and soft connective tissues. In culture, chondrogenesis is initiated very rapidly in the interdigital explants as revealed by in situ hybridization with riboprobes specific for type IIA and IIB procollagen mRNAs. The ability of interdigital tissues to form cartilage is not attributed to factors present in the serum of the culture medium as this phenomenon is also observed in serumless cultures. We have found that if all-trans retinoic acid, at concentrations of 10-50 ng/ml culture medium, were added to the explants it could inhibit chondrogenesis and promote cell death. Moreover, in some of the cultures, a single digit was left attached to the interdigital tissue. This also dramatically reduced the incidence of chondrogenesis. We have tried the determine whether the digits and ectoderm can produce a diffusible factor that can prevent cartilage from developing by culturing day 12.5 interdigital tissues in ectoderm and digit conditioned media. The ectoderm conditioned medium had no effects on interdigital growth or chondrogenesis. In contrast, the size of interdigital explants cultured in the presence of digit conditioned medium was shown to be significantly smaller than the control. These explants also produced a smaller quantity of cartilage as revealed by Alcian blue binding assay. In sum, our results showed that the fate of the interdigital tissues are not fully determined until after day 13.5. These tissues have the potentials to form cartilage and soft connective tissues. We tentatively propose that these interdigital tissues do not normally realize their histogenetic potentials because of the antichondrogenic influence of the digits and retinoic acid.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417en_US
dc.relation.ispartofDevelopmental Dynamicsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCartilage - Drug Effects - Embryology - Metabolismen_US
dc.subject.meshConnective Tissue - Drug Effects - Embryology - Metabolismen_US
dc.subject.meshEctoderm - Cytology - Metabolismen_US
dc.subject.meshEmbryonic And Fetal Development - Drug Effectsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshGestational Ageen_US
dc.subject.meshHindlimb - Drug Effects - Embryology - Metabolismen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Icren_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshPregnancyen_US
dc.subject.meshProcollagen - Geneticsen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshTretinoin - Pharmacologyen_US
dc.subject.meshWound Healingen_US
dc.titleInfluence of digits, ectoderm, and retinoic acid on chondrogenesis by mouse interdigital mesoderm in cultureen_US
dc.typeArticleen_US
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_US
dc.identifier.authorityCheah, KSE=rp00342en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7894069-
dc.identifier.scopuseid_2-s2.0-0028607402en_US
dc.identifier.hkuros1706-
dc.identifier.volume201en_US
dc.identifier.issue4en_US
dc.identifier.spage297en_US
dc.identifier.epage309en_US
dc.identifier.isiWOS:A1994QF97400001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLee, KKH=8518575700en_US
dc.identifier.scopusauthoridLi, FCH=26659479700en_US
dc.identifier.scopusauthoridYung, WT=35950828700en_US
dc.identifier.scopusauthoridKung, JLS=35834393900en_US
dc.identifier.scopusauthoridNg, JL=7202427211en_US
dc.identifier.scopusauthoridCheah, KSE=35387746200en_US

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