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Article: Neural specificity of elav expression: Defining a Drosophila promoter for directing expression to the nervous system

TitleNeural specificity of elav expression: Defining a Drosophila promoter for directing expression to the nervous system
Authors
Issue Date1994
Citation
Journal Of Neurochemistry, 1994, v. 63 n. 1, p. 41-51 How to Cite?
AbstractThe Drosophila melanogaster vital gene, embryonic lethal abnormal visual system (elav), is required for the postdeterminative development of the nervous system. Its gene product encodes an RNA binding protein that was found to be expressed in all neurons right after their birth. This specific, ubiquitous, and continuous pattern of neural expression has led to the increasingly popular use of ELAV protein as a neural-specific marker. To understand the molecular basis of this neural-specific expression, we have defined and analyzed the structure of the elav promoter. Cis-acting sequences important for conferring the neural specificity of elav expression were identified by analyzing the reporter gene expression in transformants carrying different elav-β-galactosidase fusion genes. This analysis delimits a 333-bp region (-92 to +241) that is necessary for specifying the elav pattern of nervous system expression. A 3.5-kb promoter fragment encompassing this region was designed for targeting gene expression specifically to the nervous system and would be a useful tool for the analysis of nervous system function.
Persistent Identifierhttp://hdl.handle.net/10722/147388
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYao, KMen_US
dc.contributor.authorWhite, Ken_US
dc.date.accessioned2012-05-29T06:03:21Z-
dc.date.available2012-05-29T06:03:21Z-
dc.date.issued1994en_US
dc.identifier.citationJournal Of Neurochemistry, 1994, v. 63 n. 1, p. 41-51en_US
dc.identifier.issn0022-3042en_US
dc.identifier.urihttp://hdl.handle.net/10722/147388-
dc.description.abstractThe Drosophila melanogaster vital gene, embryonic lethal abnormal visual system (elav), is required for the postdeterminative development of the nervous system. Its gene product encodes an RNA binding protein that was found to be expressed in all neurons right after their birth. This specific, ubiquitous, and continuous pattern of neural expression has led to the increasingly popular use of ELAV protein as a neural-specific marker. To understand the molecular basis of this neural-specific expression, we have defined and analyzed the structure of the elav promoter. Cis-acting sequences important for conferring the neural specificity of elav expression were identified by analyzing the reporter gene expression in transformants carrying different elav-β-galactosidase fusion genes. This analysis delimits a 333-bp region (-92 to +241) that is necessary for specifying the elav pattern of nervous system expression. A 3.5-kb promoter fragment encompassing this region was designed for targeting gene expression specifically to the nervous system and would be a useful tool for the analysis of nervous system function.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Neurochemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCentral Nervous System - Chemistry - Physiologyen_US
dc.subject.meshDna - Analysis - Geneticsen_US
dc.subject.meshDrosophila - Geneticsen_US
dc.subject.meshEnhancer Elements, Geneticen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGenes, Insecten_US
dc.subject.meshHu Paraneoplastic Encephalomyelitis Antigensen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeurons - Chemistry - Physiologyen_US
dc.subject.meshOrgan Specificityen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRibonucleoproteins - Analysis - Genetics - Physiologyen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshBeta-Galactosidase - Geneticsen_US
dc.titleNeural specificity of elav expression: Defining a Drosophila promoter for directing expression to the nervous systemen_US
dc.typeArticleen_US
dc.identifier.emailYao, KM:kmyao@hku.hken_US
dc.identifier.authorityYao, KM=rp00344en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8207445-
dc.identifier.scopuseid_2-s2.0-0028352925en_US
dc.identifier.volume63en_US
dc.identifier.issue1en_US
dc.identifier.spage41en_US
dc.identifier.epage51en_US
dc.identifier.isiWOS:A1994NT49700005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYao, KM=7403234578en_US
dc.identifier.scopusauthoridWhite, K=35569872100en_US

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