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Article: The zinc finger gene Krox20 regulates HoxB2 (Hox2.8) during hindbrain segmentation

TitleThe zinc finger gene Krox20 regulates HoxB2 (Hox2.8) during hindbrain segmentation
Authors
Issue Date1993
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell
Citation
Cell, 1993, v. 72 n. 2, p. 183-196 How to Cite?
AbstractThe zinc finger gene Krox20 and many Hox homeobox genes are expressed in segment-restricted domains in the hindbrain. The restricted expression patterns appear before morphological segmentation, suggesting that these transcription factors may play an early role in the establishment and identity of rhombomeric segments. In this paper, we show that the HoxB2 (Hox2.8) gene is normally upregulated in rhombomeres (r) 3, 4, and 5, and we identify an enhancer region upstream of the gene that imposes r3/r5 expression in transgenic mice. This enhancer contains three Krox20-binding sites required in vitro for complex formation with Krox20 protein and in vivo for rhombomere-restricted expression. In transgenic mice, Krox20 expressed in ectopic domains can transactivate a reporter construct containing the HoxB2 r3/r5 enhancer. These data demonstrate that Krox20 is a part of the upstream transcriptional cascade that directly regulates HoxB2 expression during hindbrain segmentation.
Persistent Identifierhttp://hdl.handle.net/10722/147385
ISSN
2015 Impact Factor: 28.71
2015 SCImago Journal Rankings: 28.188
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMai Har Shamen_US
dc.contributor.authorVesque, Cen_US
dc.contributor.authorNonchev, Sen_US
dc.contributor.authorMarshall, Hen_US
dc.contributor.authorFrain, Men_US
dc.contributor.authorGupta, RDen_US
dc.contributor.authorWhiting, Jen_US
dc.contributor.authorWilkinson, Den_US
dc.contributor.authorCharnay, Pen_US
dc.contributor.authorKrumlauf, Ren_US
dc.date.accessioned2012-05-29T06:03:20Z-
dc.date.available2012-05-29T06:03:20Z-
dc.date.issued1993en_US
dc.identifier.citationCell, 1993, v. 72 n. 2, p. 183-196en_US
dc.identifier.issn0092-8674en_US
dc.identifier.urihttp://hdl.handle.net/10722/147385-
dc.description.abstractThe zinc finger gene Krox20 and many Hox homeobox genes are expressed in segment-restricted domains in the hindbrain. The restricted expression patterns appear before morphological segmentation, suggesting that these transcription factors may play an early role in the establishment and identity of rhombomeric segments. In this paper, we show that the HoxB2 (Hox2.8) gene is normally upregulated in rhombomeres (r) 3, 4, and 5, and we identify an enhancer region upstream of the gene that imposes r3/r5 expression in transgenic mice. This enhancer contains three Krox20-binding sites required in vitro for complex formation with Krox20 protein and in vivo for rhombomere-restricted expression. In transgenic mice, Krox20 expressed in ectopic domains can transactivate a reporter construct containing the HoxB2 r3/r5 enhancer. These data demonstrate that Krox20 is a part of the upstream transcriptional cascade that directly regulates HoxB2 expression during hindbrain segmentation.en_US
dc.languageengen_US
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cellen_US
dc.relation.ispartofCellen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBrain - Embryologyen_US
dc.subject.meshDna - Genetics - Metabolismen_US
dc.subject.meshDna Probesen_US
dc.subject.meshDna-Binding Proteins - Genetics - Metabolismen_US
dc.subject.meshEarly Growth Response Protein 2en_US
dc.subject.meshEmbryonic And Fetal Developmenten_US
dc.subject.meshEnhancer Elements, Geneticen_US
dc.subject.meshGenes, Homeoboxen_US
dc.subject.meshGenes, Regulatoren_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutagenesis, Site-Directeden_US
dc.subject.meshOligodeoxyribonucleotidesen_US
dc.subject.meshRna Probesen_US
dc.subject.meshRhombencephalon - Embryologyen_US
dc.subject.meshTranscription Factors - Genetics - Metabolismen_US
dc.subject.meshZinc Fingers - Geneticsen_US
dc.titleThe zinc finger gene Krox20 regulates HoxB2 (Hox2.8) during hindbrain segmentationen_US
dc.typeArticleen_US
dc.identifier.emailMai Har Sham:mhsham@hkucc.hku.hken_US
dc.identifier.authorityMai Har Sham=rp00380en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0092-8674(93)90659-Een_US
dc.identifier.pmid8093858-
dc.identifier.scopuseid_2-s2.0-0027536252en_US
dc.identifier.volume72en_US
dc.identifier.issue2en_US
dc.identifier.spage183en_US
dc.identifier.epage196en_US
dc.identifier.isiWOS:A1993KK03800004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMai Har Sham=7003729109en_US
dc.identifier.scopusauthoridVesque, C=6603365734en_US
dc.identifier.scopusauthoridNonchev, S=6603818489en_US
dc.identifier.scopusauthoridMarshall, H=7102515675en_US
dc.identifier.scopusauthoridFrain, M=6603843656en_US
dc.identifier.scopusauthoridGupta, RD=7501323525en_US
dc.identifier.scopusauthoridWhiting, J=7102847929en_US
dc.identifier.scopusauthoridWilkinson, D=7401870302en_US
dc.identifier.scopusauthoridCharnay, P=7004489856en_US
dc.identifier.scopusauthoridKrumlauf, R=7006242495en_US

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