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Article: The interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression

TitleThe interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression
Authors
Issue Date1993
PublisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/
Citation
Genes And Development, 1993, v. 7 n. 4, p. 694-704 How to Cite?
AbstractThe product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions within the second intron of the gene cooperate with one another to generate the wild-type level and pattern of dpp transcription. These regions contain both generalized enhancer elements as well as ventral-specific repressor elements. Placed within the context of heterologous promoters, the intron retains its ability to direct general activation and ventral repression. The ventral specific repression of dpp transcription is directly mediated by binding sites for the dorsal (dl) morphogen in the repressor elements. In contrast with the zerknult (zen) ventral repressor element, which contains a few high-affinity dl-binding sites, dpp contains multiple relatively low-affinity sites that function together to bring about complete ventral repression. Because dpp and zen have nearly coincident early expression domains, these results indicate that the same boundary of repression can be specified by dl-binding sites of different affinity. We discuss the possibility that unknown factors interact with dl protein to determine the domain of dl-mediated repression.
Persistent Identifierhttp://hdl.handle.net/10722/147376
ISSN
2015 Impact Factor: 10.042
2015 SCImago Journal Rankings: 11.812
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHuang, JDen_US
dc.contributor.authorSchwyter, DHen_US
dc.contributor.authorShirokawa, JMen_US
dc.contributor.authorCourey, AJen_US
dc.date.accessioned2012-05-29T06:03:16Z-
dc.date.available2012-05-29T06:03:16Z-
dc.date.issued1993en_US
dc.identifier.citationGenes And Development, 1993, v. 7 n. 4, p. 694-704en_US
dc.identifier.issn0890-9369en_US
dc.identifier.urihttp://hdl.handle.net/10722/147376-
dc.description.abstractThe product of the zygotically active decapentaplegic (dpp) gene appears to function as a morphogen that specifies positional information in the dorsal half of the Drosophila embryo. The dorsal-specific transcription of dpp is the key step in establishing a morphogen gradient. We demonstrate here that multiple regions within the second intron of the gene cooperate with one another to generate the wild-type level and pattern of dpp transcription. These regions contain both generalized enhancer elements as well as ventral-specific repressor elements. Placed within the context of heterologous promoters, the intron retains its ability to direct general activation and ventral repression. The ventral specific repression of dpp transcription is directly mediated by binding sites for the dorsal (dl) morphogen in the repressor elements. In contrast with the zerknult (zen) ventral repressor element, which contains a few high-affinity dl-binding sites, dpp contains multiple relatively low-affinity sites that function together to bring about complete ventral repression. Because dpp and zen have nearly coincident early expression domains, these results indicate that the same boundary of repression can be specified by dl-binding sites of different affinity. We discuss the possibility that unknown factors interact with dl protein to determine the domain of dl-mediated repression.en_US
dc.languageengen_US
dc.publisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/en_US
dc.relation.ispartofGenes and Developmenten_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBlastodermen_US
dc.subject.meshCell Differentiation - Geneticsen_US
dc.subject.meshDna - Analysisen_US
dc.subject.meshDna-Binding Proteins - Geneticsen_US
dc.subject.meshDrosophila - Geneticsen_US
dc.subject.meshDrosophila Proteinsen_US
dc.subject.meshEnhancer Elements, Geneticen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGenes, Insecten_US
dc.subject.meshInsect Hormones - Geneticsen_US
dc.subject.meshIntronsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMorphogenesis - Geneticsen_US
dc.subject.meshNuclear Proteins - Genetics - Physiologyen_US
dc.subject.meshPhosphoproteinsen_US
dc.subject.meshRegulatory Sequences, Nucleic Acid - Geneticsen_US
dc.subject.meshRepressor Proteins - Genetics - Physiologyen_US
dc.subject.meshTranscription Factors - Geneticsen_US
dc.titleThe interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expressionen_US
dc.typeArticleen_US
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_US
dc.identifier.authorityHuang, JD=rp00451en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8458580-
dc.identifier.scopuseid_2-s2.0-0027189976en_US
dc.identifier.volume7en_US
dc.identifier.issue4en_US
dc.identifier.spage694en_US
dc.identifier.epage704en_US
dc.identifier.isiWOS:A1993KW75800015-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHuang, JD=8108660600en_US
dc.identifier.scopusauthoridSchwyter, DH=6507993446en_US
dc.identifier.scopusauthoridShirokawa, JM=6602300857en_US
dc.identifier.scopusauthoridCourey, AJ=7003350876en_US

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