Article: Epitope specificity of antibodies to type II collagen in rheumatoid arthritis and systemic lupus erythematosus

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TitleEpitope specificity of antibodies to type II collagen in rheumatoid arthritis and systemic lupus erythematosus
AuthorsRowley, MJ1
Mackay, IR1
Brand, CA1
Bateman, JF1
Chan, D1
Issue Date1992
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00296/index.htm
CitationRheumatology International, 1992, v. 12 n. 2, p. 65-69 [How to Cite?]
DOI: http://dx.doi.org/10.1007/BF00300979
AbstractAntibodies to human type II collagen were examined in the sera of 105 patients with rheumatoid arthritis (RA), 44 patients with systemic lupus erythematosus (SEF) and 11 patients who fulfilled the criteria of both diseases (RA-SLE overlap), using a solid-phase radioimmunoassay (RIA). The frequencies of antibodies to native and denatured human type II collagen were 20% and 27% in RA, 14% and 16% in SLE, and 45% and 36% in RA-SLE overlap. The specificity of the antibodies was further examined by inhibition with native and denatured type II collagen, by immunoblotting on native and denatured type II collagen, and by immunoblotting on cyanogen-bromide derived polypeptides of type II collagen. We could not identify any disease-specific patterns of reactivity. Thus, in the three disease groups the antibody response was polyclonal; there were antibody populations that reacted with native and/or denatured collagen, and epitopes could be assigned to at least three CB peptides, CB10.5, CB11 and CB8.
ISSN0172-8172
2011 Impact Factor: 1.885
2011 SCImago Journal Rankings: 0.104
DOIhttp://dx.doi.org/10.1007/BF00300979
ISI Accession Number IDWOS:A1992JA83300005
DC Field
Value
dc.contributor.authorRowley, MJ
dc.contributor.authorMackay, IR
dc.contributor.authorBrand, CA
dc.contributor.authorBateman, JF
dc.contributor.authorChan, D
dc.date.accessioned2012-05-29T06:03:14Z
dc.date.available2012-05-29T06:03:14Z
dc.date.issued1992
dc.description.abstractAntibodies to human type II collagen were examined in the sera of 105 patients with rheumatoid arthritis (RA), 44 patients with systemic lupus erythematosus (SEF) and 11 patients who fulfilled the criteria of both diseases (RA-SLE overlap), using a solid-phase radioimmunoassay (RIA). The frequencies of antibodies to native and denatured human type II collagen were 20% and 27% in RA, 14% and 16% in SLE, and 45% and 36% in RA-SLE overlap. The specificity of the antibodies was further examined by inhibition with native and denatured type II collagen, by immunoblotting on native and denatured type II collagen, and by immunoblotting on cyanogen-bromide derived polypeptides of type II collagen. We could not identify any disease-specific patterns of reactivity. Thus, in the three disease groups the antibody response was polyclonal; there were antibody populations that reacted with native and/or denatured collagen, and epitopes could be assigned to at least three CB peptides, CB10.5, CB11 and CB8.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationRheumatology International, 1992, v. 12 n. 2, p. 65-69 [How to Cite?]
DOI: http://dx.doi.org/10.1007/BF00300979
dc.identifier.doihttp://dx.doi.org/10.1007/BF00300979
dc.identifier.epage69
dc.identifier.isiWOS:A1992JA83300005
dc.identifier.issn0172-8172
2011 Impact Factor: 1.885
2011 SCImago Journal Rankings: 0.104
dc.identifier.issue2
dc.identifier.pmid1384102
dc.identifier.scopuseid_2-s2.0-0026659023
dc.identifier.spage65
dc.identifier.urihttp://hdl.handle.net/10722/147370
dc.identifier.volume12
dc.languageeng
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00296/index.htm
dc.publisher.placeGermany
dc.relation.ispartofRheumatology International
dc.subject.meshAntibodies - Analysis - Immunology
dc.subject.meshAntibody Specificity
dc.subject.meshArthritis, Rheumatoid - Blood - Diagnosis - Immunology
dc.subject.meshBiological Markers - Blood
dc.subject.meshCollagen - Immunology
dc.subject.meshEpitopes - Immunology
dc.subject.meshHumans
dc.subject.meshImmunoblotting
dc.subject.meshLupus Erythematosus, Systemic - Blood - Diagnosis - Immunology
dc.subject.meshRadioimmunoassay
dc.titleEpitope specificity of antibodies to type II collagen in rheumatoid arthritis and systemic lupus erythematosus
dc.typeArticle
Author Affiliations
  1. Monash University