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Article: Analysis of the murine Hox-2.7 gene: Conserved alternative transcripts with differential distributions in the nervous system and the potential for shared regulatory regions

TitleAnalysis of the murine Hox-2.7 gene: Conserved alternative transcripts with differential distributions in the nervous system and the potential for shared regulatory regions
Authors
Issue Date1992
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.html
Citation
Embo Journal, 1992, v. 11 n. 5, p. 1825-1836 How to Cite?
AbstractIn this study we have investigated the organization and regulation of the mouse Hox-2.7 gene. There are several alternative transcripts some of which are conserved between mouse and humans. By Northern and in situ analysis we are able to identify at least three types of transcripts which are different in size and splicing pattern and have distinctly different boundaries of expression in the nervous system. One subset of the endogenous transcripts has a boundary of expression that corresponds to the adjacent Hox-2.8 gene instead of Hox-2.7. In another type of transcript there is an alternative reading frame which predicts a protein that has homology to an enzyme ATPase and suggests that a non-homeobox containing gene may be located in the Hox-2 cluster. A Hox-2.7-lacZ transgene is expressed in a similar pattern to the endogenous gene in that spatially-restricted domains of expression are seen in the branchial arches, neural tube, paraxial mesoderm (somites), cranial ganglia, neural crest and gut. However, the anterior boundaries of transgene expression only correspond to the subset of Hox-2.7 transcripts which map to the Hox-2.8 boundary. The proximity of a Hox-2.7 promoter to regions which regulate the adjacent Hox-2.6 gene and the expression of transgenic and endogenous transcripts in a Hox-2.8 pattern, suggest that regulatory elements may be shared by neighbouring genes to establish the complete expression pattern.
Persistent Identifierhttp://hdl.handle.net/10722/147367
ISSN
2015 Impact Factor: 9.643
2015 SCImago Journal Rankings: 7.450
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSham, MHen_US
dc.contributor.authorHunt, Pen_US
dc.contributor.authorNonchev, Sen_US
dc.contributor.authorPapalopulu, Nen_US
dc.contributor.authorGraham, Aen_US
dc.contributor.authorBoncinelli, Een_US
dc.contributor.authorKrumlauf, Ren_US
dc.date.accessioned2012-05-29T06:03:13Z-
dc.date.available2012-05-29T06:03:13Z-
dc.date.issued1992en_US
dc.identifier.citationEmbo Journal, 1992, v. 11 n. 5, p. 1825-1836en_US
dc.identifier.issn0261-4189en_US
dc.identifier.urihttp://hdl.handle.net/10722/147367-
dc.description.abstractIn this study we have investigated the organization and regulation of the mouse Hox-2.7 gene. There are several alternative transcripts some of which are conserved between mouse and humans. By Northern and in situ analysis we are able to identify at least three types of transcripts which are different in size and splicing pattern and have distinctly different boundaries of expression in the nervous system. One subset of the endogenous transcripts has a boundary of expression that corresponds to the adjacent Hox-2.8 gene instead of Hox-2.7. In another type of transcript there is an alternative reading frame which predicts a protein that has homology to an enzyme ATPase and suggests that a non-homeobox containing gene may be located in the Hox-2 cluster. A Hox-2.7-lacZ transgene is expressed in a similar pattern to the endogenous gene in that spatially-restricted domains of expression are seen in the branchial arches, neural tube, paraxial mesoderm (somites), cranial ganglia, neural crest and gut. However, the anterior boundaries of transgene expression only correspond to the subset of Hox-2.7 transcripts which map to the Hox-2.8 boundary. The proximity of a Hox-2.7 promoter to regions which regulate the adjacent Hox-2.6 gene and the expression of transgenic and endogenous transcripts in a Hox-2.8 pattern, suggest that regulatory elements may be shared by neighbouring genes to establish the complete expression pattern.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.htmlen_US
dc.relation.ispartofEMBO Journalen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNervous System - Embryology - Metabolismen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshOpen Reading Framesen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRna Splicingen_US
dc.subject.meshRegulatory Sequences, Nucleic Aciden_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleAnalysis of the murine Hox-2.7 gene: Conserved alternative transcripts with differential distributions in the nervous system and the potential for shared regulatory regionsen_US
dc.typeArticleen_US
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_US
dc.identifier.authoritySham, MH=rp00380en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1582411-
dc.identifier.scopuseid_2-s2.0-0026520839en_US
dc.identifier.volume11en_US
dc.identifier.issue5en_US
dc.identifier.spage1825en_US
dc.identifier.epage1836en_US
dc.identifier.isiWOS:A1992HT23400018-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSham, MH=7003729109en_US
dc.identifier.scopusauthoridHunt, P=7202324344en_US
dc.identifier.scopusauthoridNonchev, S=6603818489en_US
dc.identifier.scopusauthoridPapalopulu, N=7003620605en_US
dc.identifier.scopusauthoridGraham, A=7402187840en_US
dc.identifier.scopusauthoridBoncinelli, E=7005365323en_US
dc.identifier.scopusauthoridKrumlauf, R=7006242495en_US

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