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Article: The mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15

TitleThe mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15
Authors
Issue Date1991
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00335/
Citation
Mammalian Genome, 1991, v. 1 n. 3, p. 171-183 How to Cite?
AbstractType II collagen is the major extracellular matrix component of cartilage and correct expression of the α1(II) collagen gene is important for vertebrate skeletal development. In order to provide the basis for studying the control of type II collagen gene expression in embryogenesis and in mouse models of human connective tissue disease, the complete mouse Col2-a1 gene has been isolated in a single cosmid clone, cosMcol.2, and partially characterized. The gene is approximately 30 kb and is highly conserved in exon/intron structure and nucleotide and amino acid sequence (>80% homology) when compared with the human, rat, bovine and chicken equivalents. A high degree of conservation was also found in the 5′ flanking region of the rat, human and mouse α1(II) collagen genes, including the presence of several G+C and C+T rich, direct repeat motifs. The sites of transcription start, termination codon and polyadenylation have also been identified. Unlike chicken, bovine and human, where polyA attachment is at a single site, for the mouse Col2a-1 gene two polyadenylation sites are utilized. Col2a-1 has also been localized by interspecies backcross analysis to the central portion of mouse Chromosome (Chr) 15, approximately 8 centiMorgans (cM) proximal of Int-1 and 18 cM distal of Myc. Col2a-1 is therefore included in a linkage group which is conserved on human Chr 12q. © 1991 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/147365
ISSN
2015 Impact Factor: 2.078
2015 SCImago Journal Rankings: 2.082
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheah, KSEen_US
dc.contributor.authorAu, PKCen_US
dc.contributor.authorLau, ETen_US
dc.contributor.authorLittle, PFRen_US
dc.contributor.authorStubbs, Len_US
dc.date.accessioned2012-05-29T06:03:12Z-
dc.date.available2012-05-29T06:03:12Z-
dc.date.issued1991en_US
dc.identifier.citationMammalian Genome, 1991, v. 1 n. 3, p. 171-183en_US
dc.identifier.issn0938-8990en_US
dc.identifier.urihttp://hdl.handle.net/10722/147365-
dc.description.abstractType II collagen is the major extracellular matrix component of cartilage and correct expression of the α1(II) collagen gene is important for vertebrate skeletal development. In order to provide the basis for studying the control of type II collagen gene expression in embryogenesis and in mouse models of human connective tissue disease, the complete mouse Col2-a1 gene has been isolated in a single cosmid clone, cosMcol.2, and partially characterized. The gene is approximately 30 kb and is highly conserved in exon/intron structure and nucleotide and amino acid sequence (>80% homology) when compared with the human, rat, bovine and chicken equivalents. A high degree of conservation was also found in the 5′ flanking region of the rat, human and mouse α1(II) collagen genes, including the presence of several G+C and C+T rich, direct repeat motifs. The sites of transcription start, termination codon and polyadenylation have also been identified. Unlike chicken, bovine and human, where polyA attachment is at a single site, for the mouse Col2a-1 gene two polyadenylation sites are utilized. Col2a-1 has also been localized by interspecies backcross analysis to the central portion of mouse Chromosome (Chr) 15, approximately 8 centiMorgans (cM) proximal of Int-1 and 18 cM distal of Myc. Col2a-1 is therefore included in a linkage group which is conserved on human Chr 12q. © 1991 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00335/en_US
dc.relation.ispartofMammalian Genomeen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshCollagen - Geneticsen_US
dc.subject.meshCrosses, Geneticen_US
dc.subject.meshMice - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPoly A - Metabolismen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshVertebrates - Geneticsen_US
dc.titleThe mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15en_US
dc.typeArticleen_US
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_US
dc.identifier.authorityCheah, KSE=rp00342en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00351064en_US
dc.identifier.pmid1797232en_US
dc.identifier.scopuseid_2-s2.0-0026311236en_US
dc.identifier.volume1en_US
dc.identifier.issue3en_US
dc.identifier.spage171en_US
dc.identifier.epage183en_US
dc.identifier.isiWOS:A1991GE80700006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCheah, KSE=35387746200en_US
dc.identifier.scopusauthoridAu, PKC=36789243400en_US
dc.identifier.scopusauthoridLau, ET=7103086081en_US
dc.identifier.scopusauthoridLittle, PFR=7201984979en_US
dc.identifier.scopusauthoridStubbs, L=7006540193en_US

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