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Article: Functional analysis of the Drosophila twist promoter reveals a dorsal-binding ventral activator region

TitleFunctional analysis of the Drosophila twist promoter reveals a dorsal-binding ventral activator region
Authors
Issue Date1991
PublisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/
Citation
Genes And Development, 1991, v. 5 n. 10, p. 1892-1901 How to Cite?
Abstracttwist is one of the earliest expressed zygotically active genes required for dorsal-ventral pattern formation in the Drosophila embryo. Genetic studies suggest that this gene is activated in the ventral part of the blastoderm by maternally expressed dorsal gene product. Using P-element-mediated germ-line transformation, we have mapped a small (260 bp) dorsal-dependent ventral activator region (VAR) in the 5'-flanking region of the twist promoter that can direct the early ventral expression of a heterologous promoter. The VAR contains binding sites for a number of proteins present in extracts of Drosophila embryos. One of these sites bears homology to known binding sites for the dorsal transcription factor and is specifically bound by bacterially expressed dorsal protein. Furthermore, a 37-bp deletion that removes the dorsal-binding sequences abolishes the ventral-specific activity of the twist promoter constructs. Our data also show that additional sequences within the VAR are required to render the dorsal-binding sites functional. Finally, reverse genetic and biochemical data suggest that the transcription factor, encoded by the zeste gene may help control the overall level, if not the pattern, of twist expression.
Persistent Identifierhttp://hdl.handle.net/10722/147363
ISSN
2015 Impact Factor: 10.042
2015 SCImago Journal Rankings: 11.812
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPan, Den_US
dc.contributor.authorHuang, JDen_US
dc.contributor.authorCourey, AJen_US
dc.date.accessioned2012-05-29T06:03:11Z-
dc.date.available2012-05-29T06:03:11Z-
dc.date.issued1991en_US
dc.identifier.citationGenes And Development, 1991, v. 5 n. 10, p. 1892-1901en_US
dc.identifier.issn0890-9369en_US
dc.identifier.urihttp://hdl.handle.net/10722/147363-
dc.description.abstracttwist is one of the earliest expressed zygotically active genes required for dorsal-ventral pattern formation in the Drosophila embryo. Genetic studies suggest that this gene is activated in the ventral part of the blastoderm by maternally expressed dorsal gene product. Using P-element-mediated germ-line transformation, we have mapped a small (260 bp) dorsal-dependent ventral activator region (VAR) in the 5'-flanking region of the twist promoter that can direct the early ventral expression of a heterologous promoter. The VAR contains binding sites for a number of proteins present in extracts of Drosophila embryos. One of these sites bears homology to known binding sites for the dorsal transcription factor and is specifically bound by bacterially expressed dorsal protein. Furthermore, a 37-bp deletion that removes the dorsal-binding sequences abolishes the ventral-specific activity of the twist promoter constructs. Our data also show that additional sequences within the VAR are required to render the dorsal-binding sites functional. Finally, reverse genetic and biochemical data suggest that the transcription factor, encoded by the zeste gene may help control the overall level, if not the pattern, of twist expression.en_US
dc.languageengen_US
dc.publisherCold Spring Harbor Laboratory Press. The Journal's web site is located at http://genesdev.cshlp.org/en_US
dc.relation.ispartofGenes and Developmenten_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshChromosome Deletionen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshDna Transposable Elementsen_US
dc.subject.meshDeoxyribonuclease Ien_US
dc.subject.meshDrosophila - Embryology - Geneticsen_US
dc.subject.meshDrosophila Proteinsen_US
dc.subject.meshEmbryo, Nonmammalian - Physiologyen_US
dc.subject.meshEmbryonic And Fetal Developmenten_US
dc.subject.meshGene Expressionen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutagenesis, Insertionalen_US
dc.subject.meshNuclear Proteins - Genetics - Metabolismen_US
dc.subject.meshPhosphoproteinsen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshTranscription Factors - Genetics - Metabolismen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleFunctional analysis of the Drosophila twist promoter reveals a dorsal-binding ventral activator regionen_US
dc.typeArticleen_US
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_US
dc.identifier.authorityHuang, JD=rp00451en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1655573-
dc.identifier.scopuseid_2-s2.0-0026072242en_US
dc.identifier.volume5en_US
dc.identifier.issue10en_US
dc.identifier.spage1892en_US
dc.identifier.epage1901en_US
dc.identifier.isiWOS:A1991GJ65400016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPan, D=7202084898en_US
dc.identifier.scopusauthoridHuang, JD=8108660600en_US
dc.identifier.scopusauthoridCourey, AJ=7003350876en_US

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