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Article: Organizational analysis of elav gene and functional analysis of ELAV protein of Drosophila melanogaster and Drosophila virilis

TitleOrganizational analysis of elav gene and functional analysis of ELAV protein of Drosophila melanogaster and Drosophila virilis
Authors
Issue Date1991
Citation
Molecular And Cellular Biology, 1991, v. 11 n. 6, p. 2994-3000 How to Cite?
AbstractDrosophila virilis genomic DNA corresponding to the D. melanogaster embryonic lethal abnormal visual system (elav) locus was cloned. DNA sequence analysis of a 3.8-kb genomic piece allowed identification of (i) an open reading frame (ORF) with striking homology to the previously identified D. melanogaster ORF and (ii) conserved sequence elements of possible regulatory relevance within and flanking the second intron. Conceptual translation of the D. virilis ORF predicts a 519-amino-acid-long ribonucleoprotein consensus sequence-type protein. Similar to D. melanogaster ELAV protein, it contains three tandem RNA-binding domains and an alanine/glutamine-rich amino-terminal region. The sequence throughout the RNA-binding domains, comprising the carboxy-terminal 346 amino acids, shows an extraordinary 100% identity at the amino acid level, indicating a strong structural constraint for this functional domain. The amino-terminal region is 36 amino acids longer in D. virilis, and the conservation is 66%. In in vivo functional tests, the D. virilis ORF was indistinguishable from the D. melanogaster ORF. Furthermore, a D. melanogaster ORF encoding an ELAV protein with a 40-amino-acid deletion within the alanine/glutamine-rich region was also able to supply elav function in vivo. Thus, the divergence of the amino-terminal region of the ELAV protein reflects lowered functional constraint rather than species-specific functional specification.
Persistent Identifierhttp://hdl.handle.net/10722/147360
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYao, KMen_US
dc.contributor.authorWhite, Ken_US
dc.date.accessioned2012-05-29T06:03:10Z-
dc.date.available2012-05-29T06:03:10Z-
dc.date.issued1991en_US
dc.identifier.citationMolecular And Cellular Biology, 1991, v. 11 n. 6, p. 2994-3000en_US
dc.identifier.issn0270-7306en_US
dc.identifier.urihttp://hdl.handle.net/10722/147360-
dc.description.abstractDrosophila virilis genomic DNA corresponding to the D. melanogaster embryonic lethal abnormal visual system (elav) locus was cloned. DNA sequence analysis of a 3.8-kb genomic piece allowed identification of (i) an open reading frame (ORF) with striking homology to the previously identified D. melanogaster ORF and (ii) conserved sequence elements of possible regulatory relevance within and flanking the second intron. Conceptual translation of the D. virilis ORF predicts a 519-amino-acid-long ribonucleoprotein consensus sequence-type protein. Similar to D. melanogaster ELAV protein, it contains three tandem RNA-binding domains and an alanine/glutamine-rich amino-terminal region. The sequence throughout the RNA-binding domains, comprising the carboxy-terminal 346 amino acids, shows an extraordinary 100% identity at the amino acid level, indicating a strong structural constraint for this functional domain. The amino-terminal region is 36 amino acids longer in D. virilis, and the conservation is 66%. In in vivo functional tests, the D. virilis ORF was indistinguishable from the D. melanogaster ORF. Furthermore, a D. melanogaster ORF encoding an ELAV protein with a 40-amino-acid deletion within the alanine/glutamine-rich region was also able to supply elav function in vivo. Thus, the divergence of the amino-terminal region of the ELAV protein reflects lowered functional constraint rather than species-specific functional specification.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Biologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDna - Genetics - Isolation & Purificationen_US
dc.subject.meshDrosophila - Embryology - Geneticsen_US
dc.subject.meshDrosophila Melanogaster - Embryology - Geneticsen_US
dc.subject.meshGenes, Lethalen_US
dc.subject.meshHu Paraneoplastic Encephalomyelitis Antigensen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligonucleotide Probesen_US
dc.subject.meshOpen Reading Framesen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshRibonucleoproteins - Geneticsen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.titleOrganizational analysis of elav gene and functional analysis of ELAV protein of Drosophila melanogaster and Drosophila virilisen_US
dc.typeArticleen_US
dc.identifier.emailYao, KM:kmyao@hku.hken_US
dc.identifier.authorityYao, KM=rp00344en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1903840-
dc.identifier.scopuseid_2-s2.0-0025907944en_US
dc.identifier.volume11en_US
dc.identifier.issue6en_US
dc.identifier.spage2994en_US
dc.identifier.epage3000en_US
dc.identifier.isiWOS:A1991FM85200009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYao, KM=7403234578en_US
dc.identifier.scopusauthoridWhite, K=35569872100en_US

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