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Article: Methylation and expression of human pepsinogen genes in normal tissues and their alteration in stomach cancer

TitleMethylation and expression of human pepsinogen genes in normal tissues and their alteration in stomach cancer
Authors
Issue Date1991
Citation
Japanese Journal Of Cancer Research, 1991, v. 82 n. 6, p. 686-692 How to Cite?
AbstractIn normal human tissues, pepsinogen A mRNA was expressed only in the fundic mucosa of the stomach, whereas pepsinogen C mRNA was expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. The distributions of these mRNAs were consistent with those of pepsinogens A and C in the gastroduodenal mucosa. Methylation analysis of DNAs from normal tissues with methylation-sensitive restriction enzymes, HpaII and HhaI, revealed that pepsinogen A and C genes are hypomethylated in tissues producing pepsinogens A and C, suggesting a role of DNA methylation in the regulation of the differential expression of the genes for the two human pepsinogens during normal differentiation. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. The functional significance of the altered methylation is unknown; however, these results suggest that considerable heterogeneity of the methylation patterns occurs in human stomach cancers.
Persistent Identifierhttp://hdl.handle.net/10722/147359
ISSN
2004 Impact Factor: 2.225
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorIchinose, Men_US
dc.contributor.authorMiki, Ken_US
dc.contributor.authorWong, NSen_US
dc.contributor.authorTatematsu, Men_US
dc.contributor.authorFurihata, Cen_US
dc.contributor.authorKonishi, Ten_US
dc.contributor.authorMatsushima Tanji, MMen_US
dc.contributor.authorSano, Jen_US
dc.contributor.authorKurokawa, Ken_US
dc.contributor.authorTakahashi, Ten_US
dc.contributor.authorKageyama, Ten_US
dc.contributor.authorTang, JJNen_US
dc.contributor.authorTakahashi, Ken_US
dc.date.accessioned2012-05-29T06:03:10Z-
dc.date.available2012-05-29T06:03:10Z-
dc.date.issued1991en_US
dc.identifier.citationJapanese Journal Of Cancer Research, 1991, v. 82 n. 6, p. 686-692en_US
dc.identifier.issn0910-5050en_US
dc.identifier.urihttp://hdl.handle.net/10722/147359-
dc.description.abstractIn normal human tissues, pepsinogen A mRNA was expressed only in the fundic mucosa of the stomach, whereas pepsinogen C mRNA was expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. The distributions of these mRNAs were consistent with those of pepsinogens A and C in the gastroduodenal mucosa. Methylation analysis of DNAs from normal tissues with methylation-sensitive restriction enzymes, HpaII and HhaI, revealed that pepsinogen A and C genes are hypomethylated in tissues producing pepsinogens A and C, suggesting a role of DNA methylation in the regulation of the differential expression of the genes for the two human pepsinogens during normal differentiation. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. The functional significance of the altered methylation is unknown; however, these results suggest that considerable heterogeneity of the methylation patterns occurs in human stomach cancers.en_US
dc.languageengen_US
dc.relation.ispartofJapanese Journal of Cancer Researchen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshDna - Genetics - Isolation & Purificationen_US
dc.subject.meshDna, Neoplasm - Genetics - Isolation & Purificationen_US
dc.subject.meshGastric Mucosa - Enzymologyen_US
dc.subject.meshGene Libraryen_US
dc.subject.meshHumansen_US
dc.subject.meshIntestinal Mucosa - Enzymologyen_US
dc.subject.meshIntestines - Enzymologyen_US
dc.subject.meshKidney - Enzymologyen_US
dc.subject.meshLiver - Enzymologyen_US
dc.subject.meshLung - Enzymologyen_US
dc.subject.meshMethylationen_US
dc.subject.meshMuscle, Smooth - Enzymologyen_US
dc.subject.meshPepsinogens - Geneticsen_US
dc.subject.meshRna, Messenger - Genetics - Isolation & Purificationen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshRestriction Mappingen_US
dc.subject.meshSpleen - Enzymologyen_US
dc.subject.meshStomach Neoplasms - Enzymology - Genetics - Pathologyen_US
dc.titleMethylation and expression of human pepsinogen genes in normal tissues and their alteration in stomach canceren_US
dc.typeArticleen_US
dc.identifier.emailWong, NS:nswong@hkucc.hku.hken_US
dc.identifier.authorityWong, NS=rp00340en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1906854-
dc.identifier.scopuseid_2-s2.0-0025865769en_US
dc.identifier.volume82en_US
dc.identifier.issue6en_US
dc.identifier.spage686en_US
dc.identifier.epage692en_US
dc.identifier.isiWOS:A1991FR93400011-
dc.identifier.scopusauthoridIchinose, M=7102219237en_US
dc.identifier.scopusauthoridMiki, K=7201964327en_US
dc.identifier.scopusauthoridWong, NS=7202836641en_US
dc.identifier.scopusauthoridTatematsu, M=7101635652en_US
dc.identifier.scopusauthoridFurihata, C=7005434279en_US
dc.identifier.scopusauthoridKonishi, T=35934659600en_US
dc.identifier.scopusauthoridMatsushima Tanji, MM=6504179128en_US
dc.identifier.scopusauthoridSano, J=24356967300en_US
dc.identifier.scopusauthoridKurokawa, K=35376523700en_US
dc.identifier.scopusauthoridTakahashi, T=7406460501en_US
dc.identifier.scopusauthoridKageyama, T=7101603562en_US
dc.identifier.scopusauthoridTang, JJN=7404637625en_US
dc.identifier.scopusauthoridTakahashi, K=7409420511en_US

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