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Article: Homologous pairing in duplex DNA regions and the formation of four-stranded paranemic joints promoted by RecA protein. Effects of GAP length and negative superhelicity

TitleHomologous pairing in duplex DNA regions and the formation of four-stranded paranemic joints promoted by RecA protein. Effects of GAP length and negative superhelicity
Authors
Issue Date1990
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1990, v. 265 n. 34, p. 21262-21268 How to Cite?
AbstractRecA protein catalyzes homologous pairing of partially single-stranded duplex DNA and fully duplex DNA to form stable joint molecules. We constructed circular duplex DNA with various defined gap lengths and studied the pairing reaction between the gapped substrate with fully double-stranded DNA. The reaction required a stoichiometric amount of RecA protein, and the optimal reaction was achieved at a ratio of 1 RecA monomer per 4 base pairs. The length of the gap, ranging from 141 to 1158 nucleotides, had little effect on the efficiency of homologous pairing. By using a circular gapped duplex DNA prepared from the chimeric phage M13Gori1, we were able to show the formation of nonintertwined or paranemic joints in duplex regions between the gapped and fully duplex molecules. The formation of such paranemic joints occurred efficiently and included nearly all of the DNA in the reaction mixture. The reaction required negative superhelicity, and pairing was greatly reduced with linear or nicked circular DNA. We conclude that one functional role of the single-stranded gap is for facilitating the binding of RecA protein to the duplex region of the gapped DNA. Once the nucleoprotein filament is formed, homologous pairing between the gapped and fully duplex DNA can take place anywhere along the length of the nucleoprotein complex.
Persistent Identifierhttp://hdl.handle.net/10722/147356
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChiu, SKen_US
dc.contributor.authorWong, BCen_US
dc.contributor.authorChow, SAen_US
dc.date.accessioned2012-05-29T06:03:08Z-
dc.date.available2012-05-29T06:03:08Z-
dc.date.issued1990en_US
dc.identifier.citationJournal Of Biological Chemistry, 1990, v. 265 n. 34, p. 21262-21268en_US
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/10722/147356-
dc.description.abstractRecA protein catalyzes homologous pairing of partially single-stranded duplex DNA and fully duplex DNA to form stable joint molecules. We constructed circular duplex DNA with various defined gap lengths and studied the pairing reaction between the gapped substrate with fully double-stranded DNA. The reaction required a stoichiometric amount of RecA protein, and the optimal reaction was achieved at a ratio of 1 RecA monomer per 4 base pairs. The length of the gap, ranging from 141 to 1158 nucleotides, had little effect on the efficiency of homologous pairing. By using a circular gapped duplex DNA prepared from the chimeric phage M13Gori1, we were able to show the formation of nonintertwined or paranemic joints in duplex regions between the gapped and fully duplex molecules. The formation of such paranemic joints occurred efficiently and included nearly all of the DNA in the reaction mixture. The reaction required negative superhelicity, and pairing was greatly reduced with linear or nicked circular DNA. We conclude that one functional role of the single-stranded gap is for facilitating the binding of RecA protein to the duplex region of the gapped DNA. Once the nucleoprotein filament is formed, homologous pairing between the gapped and fully duplex DNA can take place anywhere along the length of the nucleoprotein complex.en_US
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.subject.meshBacteriophage Phi X 174 - Metabolismen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshDna, Circular - Metabolism - Ultrastructureen_US
dc.subject.meshDna, Single-Stranded - Metabolism - Ultrastructureen_US
dc.subject.meshDna, Superhelical - Metabolismen_US
dc.subject.meshDna, Viral - Metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRec A Recombinases - Metabolism - Ultrastructureen_US
dc.subject.meshRestriction Mappingen_US
dc.titleHomologous pairing in duplex DNA regions and the formation of four-stranded paranemic joints promoted by RecA protein. Effects of GAP length and negative superhelicityen_US
dc.typeArticleen_US
dc.identifier.emailWong, BC:bcwwong@hkucc.hku.hken_US
dc.identifier.authorityWong, BC=rp00369en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2147430en_US
dc.identifier.scopuseid_2-s2.0-0025667693en_US
dc.identifier.volume265en_US
dc.identifier.issue34en_US
dc.identifier.spage21262en_US
dc.identifier.epage21268en_US
dc.identifier.isiWOS:A1990EL02000084-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCHiu, SK=7202291671en_US
dc.identifier.scopusauthoridWong, BC=35733052400en_US
dc.identifier.scopusauthoridChow, SA=7201827867en_US

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