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Article: Identification of plasmin as the major contaminant in immunoglobulin preparations

TitleIdentification of plasmin as the major contaminant in immunoglobulin preparations
Authors
Lau, HKFLui, AYYWong, NS
Issue Date1986
Citation
Comparative Biochemistry And Physiology -- Part B: Biochemistry And, 1986, v. 84 n. 4, p. 507-511 How to Cite?
DOI: http://dx.doi.org/10.1016/0305-0491(86)90114-8
Abstract1. 1. A proteolytic enzyme could be isolated from rabbit serum by means of DEAE cellulose, Protein A-bound Sepharose and lysine-bound Sepharose chromatographies. 2. 2. This enzyme was found to be the major protease contaminating IgG preparations of rabbit serum. 3. 3. This enzyme was identified as plasmin because it displayed an apparent Mr of 90,000 on nonreduced SDS polyacrylamide gel electrophoresis, was able to directly lyse fibrin and the chromogenic substrate H-d-Val-Leu-Lys-p-nitroanilide, and was stable after heating at 56° for 30 min but broke down at 80°. Its Km toward the chromogenic substrate was 0.35 mM, which agreed well with the published value for plasmin. © 1986.
Persistent Identifierhttp://hdl.handle.net/10722/147316
ISSN
0305-0491
ISI Accession Number ID
WOS:A1986D844700013

 

DC FieldValueLanguage
dc.contributor.authorLau, HKFen_US
dc.contributor.authorLui, AYYen_US
dc.contributor.authorWong, NSen_US
dc.date.accessioned2012-05-29T06:02:52Z-
dc.date.available2012-05-29T06:02:52Z-
dc.date.issued1986en_US
dc.identifier.citationComparative Biochemistry And Physiology -- Part B: Biochemistry And, 1986, v. 84 n. 4, p. 507-511en_US
dc.identifier.issn0305-0491en_US
dc.identifier.urihttp://hdl.handle.net/10722/147316-
dc.description.abstract1. 1. A proteolytic enzyme could be isolated from rabbit serum by means of DEAE cellulose, Protein A-bound Sepharose and lysine-bound Sepharose chromatographies. 2. 2. This enzyme was found to be the major protease contaminating IgG preparations of rabbit serum. 3. 3. This enzyme was identified as plasmin because it displayed an apparent Mr of 90,000 on nonreduced SDS polyacrylamide gel electrophoresis, was able to directly lyse fibrin and the chromogenic substrate H-d-Val-Leu-Lys-p-nitroanilide, and was stable after heating at 56° for 30 min but broke down at 80°. Its Km toward the chromogenic substrate was 0.35 mM, which agreed well with the published value for plasmin. © 1986.en_US
dc.languageengen_US
dc.relation.ispartofComparative Biochemistry and Physiology -- Part B: Biochemistry anden_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshChromatography, Affinity - Methodsen_US
dc.subject.meshChromatography, Deae-Cellulose - Methodsen_US
dc.subject.meshElectrophoresis, Disc - Methodsen_US
dc.subject.meshFibrinolysin - Analysis - Metabolismen_US
dc.subject.meshImmunodiffusionen_US
dc.subject.meshImmunoglobulins - Isolation & Purificationen_US
dc.subject.meshKineticsen_US
dc.subject.meshRabbitsen_US
dc.titleIdentification of plasmin as the major contaminant in immunoglobulin preparationsen_US
dc.typeArticleen_US
dc.identifier.emailWong, NS:nswong@hkucc.hku.hken_US
dc.identifier.authorityWong, NS=rp00340en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0305-0491(86)90114-8-
dc.identifier.pmid2944691-
dc.identifier.scopuseid_2-s2.0-0022464102en_US
dc.identifier.volume84en_US
dc.identifier.issue4en_US
dc.identifier.spage507en_US
dc.identifier.epage511en_US
dc.identifier.isiWOS:A1986D844700013-
dc.identifier.scopusauthoridLau, HKF=24441020200en_US
dc.identifier.scopusauthoridLui, AYY=36947385000en_US
dc.identifier.scopusauthoridWong, NS=7202836641en_US
dc.identifier.issnl0305-0491-

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