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Article: Demonstration of the presence of plasminogen activator in human small intestine

TitleDemonstration of the presence of plasminogen activator in human small intestine
Authors
Issue Date1984
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/thromres
Citation
Thrombosis Research, 1984, v. 33 n. 4, p. 389-399 How to Cite?
AbstractA cytosolic fraction of human small intestine was prepared. It contained esterase activity toward N-α-acetyl-lysine-methyl ester and amidolytic activities toward substrates S-2238, S-2288 and S-2251. In addition there was present a plasminogen activator activity which could cleave plasminogen to produce plasmin and the plasmin hydrolysed the same chromogenic substrates. Plasmin generation was also followed by a time-dependent hydrolysis of 125-I labeled plasminogen or monitored by fibrin-agar plate. The plasminogen activator was related to urinary urokinase immunologically. Anti-urokinase IgG cross-reacted with cytosolic fraction in double immunodiffusion. When the cytosolic fraction was electrophoresed in discontinuous polyacrylamide gel, two regions of hydrolytic activity toward the urokinase-specific substrate S-2444 were found. The activity of one of these regions could be completely inhibited by anti-urokinase while the other was not. The plasminogen activator was partially purified by ammonium sulfate precipitation and Concanavalin A-bound Sepharose chromatography.
Persistent Identifierhttp://hdl.handle.net/10722/147310
ISSN
2015 Impact Factor: 2.32
2015 SCImago Journal Rankings: 1.019
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, Nen_US
dc.contributor.authorLau, HKFen_US
dc.date.accessioned2012-05-29T06:02:50Z-
dc.date.available2012-05-29T06:02:50Z-
dc.date.issued1984en_US
dc.identifier.citationThrombosis Research, 1984, v. 33 n. 4, p. 389-399en_US
dc.identifier.issn0049-3848en_US
dc.identifier.urihttp://hdl.handle.net/10722/147310-
dc.description.abstractA cytosolic fraction of human small intestine was prepared. It contained esterase activity toward N-α-acetyl-lysine-methyl ester and amidolytic activities toward substrates S-2238, S-2288 and S-2251. In addition there was present a plasminogen activator activity which could cleave plasminogen to produce plasmin and the plasmin hydrolysed the same chromogenic substrates. Plasmin generation was also followed by a time-dependent hydrolysis of 125-I labeled plasminogen or monitored by fibrin-agar plate. The plasminogen activator was related to urinary urokinase immunologically. Anti-urokinase IgG cross-reacted with cytosolic fraction in double immunodiffusion. When the cytosolic fraction was electrophoresed in discontinuous polyacrylamide gel, two regions of hydrolytic activity toward the urokinase-specific substrate S-2444 were found. The activity of one of these regions could be completely inhibited by anti-urokinase while the other was not. The plasminogen activator was partially purified by ammonium sulfate precipitation and Concanavalin A-bound Sepharose chromatography.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/thromresen_US
dc.relation.ispartofThrombosis Researchen_US
dc.subject.meshAmides - Metabolismen_US
dc.subject.meshChromogenic Compounds - Metabolismen_US
dc.subject.meshCytosol - Enzymologyen_US
dc.subject.meshEsterases - Analysisen_US
dc.subject.meshFibrin - Metabolismen_US
dc.subject.meshFibrinolysin - Biosynthesis - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshIntestine, Small - Enzymologyen_US
dc.subject.meshPlasminogen Activators - Analysisen_US
dc.titleDemonstration of the presence of plasminogen activator in human small intestineen_US
dc.typeArticleen_US
dc.identifier.emailWong, N:nswong@hkucc.hku.hken_US
dc.identifier.authorityWong, N=rp00340en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6231745-
dc.identifier.scopuseid_2-s2.0-0021348205en_US
dc.identifier.volume33en_US
dc.identifier.issue4en_US
dc.identifier.spage389en_US
dc.identifier.epage399en_US
dc.identifier.isiWOS:A1984SF39500004-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWong, N=7202836641en_US
dc.identifier.scopusauthoridLau, HKF=24441020200en_US

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