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Article: A micropuncture and renal clearance study in the rat of the urinary excretion of heparin, chondroitin sulfate and metabolic breakdown products of connective tissue proteoglycans

TitleA micropuncture and renal clearance study in the rat of the urinary excretion of heparin, chondroitin sulfate and metabolic breakdown products of connective tissue proteoglycans
Authors
Issue Date1984
PublisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/
Citation
Clinical Science, 1984, v. 67 n. 2, p. 205-212 How to Cite?
AbstractCommercial heparin and acid glycosaminoglycans (AGAG) prepared from normal female human urine were tritiated catalytically in aqueous solution. Nasal septal chondroitin sulfate was tritiated by NaB3H4 reduction of the aldehydes produced by very limited periodate oxidation. The refined products were characterized by electrophoresis and biochemical analysis. The tritiated products were infused into Munich-Wistar rats, and their kidney clearances were measured and compared with that of inulin. The passage of heparin and chondroitin sulfate at the glomerulus was restricted, as shown by the ratios of their concentrations in Bowman's space fluid and plasma. Comparison with whole kidney urine/plasma ratios indicated that some tubular reabsorption also occurred. The low whole kidney clearance of AGAG from normal female human urine probably also resulted from restricted glomerular passage and tubular reabsorption. The low whole kidney clearances, primarily caused by restricted transglomerular movement, are discussed on the bases of (a) the molecular sizes and shapes of the AGAG, (b) an electrostatic barrier at the basement membrane and (c) binding of AGAG to plasma colloids, thus reducing plasma levels of free AGAG. The implications of these findings in (a) heparin therapy and (b) urinalysis of disorders of connective tissue metabolism are discussed.
Persistent Identifierhttp://hdl.handle.net/10722/147308
ISSN
2015 Impact Factor: 4.996
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShum, DKYen_US
dc.contributor.authorBaylis, Cen_US
dc.contributor.authorScott, JEen_US
dc.date.accessioned2012-05-29T06:02:50Z-
dc.date.available2012-05-29T06:02:50Z-
dc.date.issued1984en_US
dc.identifier.citationClinical Science, 1984, v. 67 n. 2, p. 205-212en_US
dc.identifier.issn0143-5221en_US
dc.identifier.urihttp://hdl.handle.net/10722/147308-
dc.description.abstractCommercial heparin and acid glycosaminoglycans (AGAG) prepared from normal female human urine were tritiated catalytically in aqueous solution. Nasal septal chondroitin sulfate was tritiated by NaB3H4 reduction of the aldehydes produced by very limited periodate oxidation. The refined products were characterized by electrophoresis and biochemical analysis. The tritiated products were infused into Munich-Wistar rats, and their kidney clearances were measured and compared with that of inulin. The passage of heparin and chondroitin sulfate at the glomerulus was restricted, as shown by the ratios of their concentrations in Bowman's space fluid and plasma. Comparison with whole kidney urine/plasma ratios indicated that some tubular reabsorption also occurred. The low whole kidney clearance of AGAG from normal female human urine probably also resulted from restricted glomerular passage and tubular reabsorption. The low whole kidney clearances, primarily caused by restricted transglomerular movement, are discussed on the bases of (a) the molecular sizes and shapes of the AGAG, (b) an electrostatic barrier at the basement membrane and (c) binding of AGAG to plasma colloids, thus reducing plasma levels of free AGAG. The implications of these findings in (a) heparin therapy and (b) urinalysis of disorders of connective tissue metabolism are discussed.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.clinsci.org/en_US
dc.relation.ispartofClinical Scienceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChondroitin - Analogs & Derivativesen_US
dc.subject.meshChondroitin Sulfates - Metabolism - Urineen_US
dc.subject.meshConnective Tissue - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshHeparin - Metabolism - Urineen_US
dc.subject.meshKidney - Metabolismen_US
dc.subject.meshKidney Glomerulus - Metabolismen_US
dc.subject.meshMetabolic Clearance Rateen_US
dc.subject.meshProteoglycans - Metabolism - Urineen_US
dc.subject.meshPuncturesen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.titleA micropuncture and renal clearance study in the rat of the urinary excretion of heparin, chondroitin sulfate and metabolic breakdown products of connective tissue proteoglycansen_US
dc.typeArticleen_US
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6430626-
dc.identifier.scopuseid_2-s2.0-0021263697en_US
dc.identifier.volume67en_US
dc.identifier.issue2en_US
dc.identifier.spage205en_US
dc.identifier.epage212en_US
dc.identifier.isiWOS:A1984TA08500008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridShum, DKY=7004824447en_US
dc.identifier.scopusauthoridBaylis, C=35568163500en_US
dc.identifier.scopusauthoridScott, JE=7407332569en_US

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