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Article: Analysis of the heterogeneity of human collagens by two-dimensional polyacrylamide-gel electrophoresis

TitleAnalysis of the heterogeneity of human collagens by two-dimensional polyacrylamide-gel electrophoresis
Authors
Issue Date1981
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 1981, v. 197 n. 2, p. 377-383 How to Cite?
AbstractThe heterogeneity of the CNBr-cleavage peptides of human types I, II, III and V collagens were studied by using two-dimensional electrophoresis combining nonequilibrium pH-gradient-gel electrophoresis and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Specific 'maps' were produced by the peptides obtained from the chains of each type of collagen, and most peptides had at least three charged forms of the same molecular weight. Specific 'maps' were also produced by the peptides of types I, III and V collagens from insoluble dermis and the peptides of types I and V collagens from decalcified bone. The α1 (I) CB7 and α1 (I) CB8 and the α2 CB4 peptides obtained from the type I collagens of these tissues contained the same number of charged components, but there was a relative increase in the more basic components in bone. Some aspects of the involvement of the α1 (I) CB6 and the α1 (III) CB9 peptides in cross-linkages were also studied. The recovery of the α1 (I) CB6 peptide from bone and dermis was decreased and the α1 (III) CB9 peptide was not detected in dermis. Additional peptides, which were probably cross-linked peptides involving the α1 (I) CB6 peptide, were also observed.
Persistent Identifierhttp://hdl.handle.net/10722/147302
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCole, WGen_US
dc.contributor.authorChan, Den_US
dc.date.accessioned2012-05-29T06:02:48Z-
dc.date.available2012-05-29T06:02:48Z-
dc.date.issued1981en_US
dc.identifier.citationBiochemical Journal, 1981, v. 197 n. 2, p. 377-383en_US
dc.identifier.issn0264-6021en_US
dc.identifier.urihttp://hdl.handle.net/10722/147302-
dc.description.abstractThe heterogeneity of the CNBr-cleavage peptides of human types I, II, III and V collagens were studied by using two-dimensional electrophoresis combining nonequilibrium pH-gradient-gel electrophoresis and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Specific 'maps' were produced by the peptides obtained from the chains of each type of collagen, and most peptides had at least three charged forms of the same molecular weight. Specific 'maps' were also produced by the peptides of types I, III and V collagens from insoluble dermis and the peptides of types I and V collagens from decalcified bone. The α1 (I) CB7 and α1 (I) CB8 and the α2 CB4 peptides obtained from the type I collagens of these tissues contained the same number of charged components, but there was a relative increase in the more basic components in bone. Some aspects of the involvement of the α1 (I) CB6 and the α1 (III) CB9 peptides in cross-linkages were also studied. The recovery of the α1 (I) CB6 peptide from bone and dermis was decreased and the α1 (III) CB9 peptide was not detected in dermis. Additional peptides, which were probably cross-linked peptides involving the α1 (I) CB6 peptide, were also observed.en_US
dc.languageengen_US
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_US
dc.relation.ispartofBiochemical Journalen_US
dc.subject.meshBone And Bones - Analysisen_US
dc.subject.meshChemical Phenomenaen_US
dc.subject.meshChemistryen_US
dc.subject.meshCollagenen_US
dc.subject.meshCyanogen Bromideen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshHumansen_US
dc.subject.meshPeptide Fragments - Analysisen_US
dc.subject.meshSkin - Analysisen_US
dc.titleAnalysis of the heterogeneity of human collagens by two-dimensional polyacrylamide-gel electrophoresisen_US
dc.typeArticleen_US
dc.identifier.emailChan, D:chand@hkucc.hku.hken_US
dc.identifier.authorityChan, D=rp00540en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7325961-
dc.identifier.scopuseid_2-s2.0-0019795388en_US
dc.identifier.volume197en_US
dc.identifier.issue2en_US
dc.identifier.spage377en_US
dc.identifier.epage383en_US
dc.identifier.isiWOS:A1981MA67700016-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridCole, WG=7201518727en_US
dc.identifier.scopusauthoridChan, D=7402216545en_US

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