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Article: Identification of the primary translation product of elastin mRNA

TitleIdentification of the primary translation product of elastin mRNA
Authors
Issue Date1981
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03008207.asp
Citation
Connective Tissue Research, 1981, v. 8 n. 3-4, p. 205-208 How to Cite?
AbstractPoly (A) RNA was prepared from matrix free cells derived from the major thoracic arteries of 17-day old chick embryos. This was translated in a messenger-dependent reticulocyte cell-free system in the presence of [35S]-methionine, [3H]-proline or [3H]-valine. The translation products were analyzed by SDS polyacrylamide gel electrophoresis and fluorography and elastin-related products were immunoprecipitated by the addition of anti-elastin sheep antiserum in the presence of proteinase inhibitors and the precipitate analyzed by the same technique. The elastin-related products were found to consist of a doublet of two closely related polypeptides (mol. wts. 70,000 and 73,000) comigrating with a tropoelastin standard. Unhydroxylated proα1 and proα2 collagen polypeptides were also found, but no elasting related product corresponding to proelastin. It is concluded that the primary translation product of elastin mRNA consists of two polypeptides between 70,000 and 73,000 mol wt which are closely related in size and immunoreactivity to tropoelastin.
Persistent Identifierhttp://hdl.handle.net/10722/147301
ISSN
2015 Impact Factor: 1.411
2015 SCImago Journal Rankings: 0.622
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheah, KSEen_US
dc.contributor.authorGrant, MEen_US
dc.contributor.authorJackson, DSen_US
dc.date.accessioned2012-05-29T06:02:47Z-
dc.date.available2012-05-29T06:02:47Z-
dc.date.issued1981en_US
dc.identifier.citationConnective Tissue Research, 1981, v. 8 n. 3-4, p. 205-208en_US
dc.identifier.issn0300-8207en_US
dc.identifier.urihttp://hdl.handle.net/10722/147301-
dc.description.abstractPoly (A) RNA was prepared from matrix free cells derived from the major thoracic arteries of 17-day old chick embryos. This was translated in a messenger-dependent reticulocyte cell-free system in the presence of [35S]-methionine, [3H]-proline or [3H]-valine. The translation products were analyzed by SDS polyacrylamide gel electrophoresis and fluorography and elastin-related products were immunoprecipitated by the addition of anti-elastin sheep antiserum in the presence of proteinase inhibitors and the precipitate analyzed by the same technique. The elastin-related products were found to consist of a doublet of two closely related polypeptides (mol. wts. 70,000 and 73,000) comigrating with a tropoelastin standard. Unhydroxylated proα1 and proα2 collagen polypeptides were also found, but no elasting related product corresponding to proelastin. It is concluded that the primary translation product of elastin mRNA consists of two polypeptides between 70,000 and 73,000 mol wt which are closely related in size and immunoreactivity to tropoelastin.en_US
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/03008207.aspen_US
dc.relation.ispartofConnective Tissue Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAorta, Thoracic - Metabolismen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshElastin - Geneticsen_US
dc.subject.meshKineticsen_US
dc.subject.meshMethionine - Metabolismen_US
dc.subject.meshPeptide Biosynthesisen_US
dc.subject.meshPoly A - Metabolismen_US
dc.subject.meshProline - Metabolismen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRna - Metabolismen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshReticulocytes - Metabolismen_US
dc.subject.meshTendons - Metabolismen_US
dc.subject.meshValine - Metabolismen_US
dc.titleIdentification of the primary translation product of elastin mRNAen_US
dc.typeArticleen_US
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_US
dc.identifier.authorityCheah, KSE=rp00342en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6167404en_US
dc.identifier.scopuseid_2-s2.0-0019466121en_US
dc.identifier.volume8en_US
dc.identifier.issue3-4en_US
dc.identifier.spage205en_US
dc.identifier.epage208en_US
dc.identifier.isiWOS:A1981LZ06400013-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridCheah, KSE=35387746200en_US
dc.identifier.scopusauthoridGrant, ME=7401439700en_US
dc.identifier.scopusauthoridJackson, DS=7404289284en_US

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