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Article: Transcription and translation of phloem protein (PP2) during phloem differentiation in Cucurbita maxima

TitleTranscription and translation of phloem protein (PP2) during phloem differentiation in Cucurbita maxima
Authors
KeywordsComplementary Dna Cloning
Cucurbita (Phloem Differentiation)
Lectin
Phloem Differentiation
Phloem Protein (Transcription, Translation)
Issue Date1987
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 1987, v. 170 n. 3, p. 392-399 How to Cite?
AbstractThe synthesis of a major phloem protein, PP2, was investigated by measurement of the mRNA at various stages of phloem development in Cucurbita. Quantitative assays with immuno-electrophoresis showed that the amounts of PP2 in hypocotyls of Cucurbita seedlings increased with the age of seedlings. An increase in mRNA for PP2 during the early stages of seedling growth was also observed by immunoprecipitation of the invitro translation products of hypocotyl polyadenylated RNA. There was close timing in the variations of PP2 synthesised in vivo and in the changes in amounts of translatable PP2-mRNA during the course of seedling growth. A complementary-DNA (cDNA) library to polyadenylated RNA from hypocotyls of 3-d-old Cucurbita seedlings has been constructed. Two cDNA clones, A and B, have been identified by hybrid-release translation to be complementary to the mRNA coding for PP2. The levels of total mRNA for PP2 measured with clone A were found to increase in the first 4 d of seedling growth but decreased to lower levels in older seedlings. Regulatory controls on both transcription and modification of transcripts appeared to occur during the synthesis of PP2. © 1987 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/147292
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSham, MHen_US
dc.contributor.authorNorthcote, DHen_US
dc.date.accessioned2012-05-29T06:02:44Z-
dc.date.available2012-05-29T06:02:44Z-
dc.date.issued1987en_US
dc.identifier.citationPlanta, 1987, v. 170 n. 3, p. 392-399en_US
dc.identifier.issn0032-0935en_US
dc.identifier.urihttp://hdl.handle.net/10722/147292-
dc.description.abstractThe synthesis of a major phloem protein, PP2, was investigated by measurement of the mRNA at various stages of phloem development in Cucurbita. Quantitative assays with immuno-electrophoresis showed that the amounts of PP2 in hypocotyls of Cucurbita seedlings increased with the age of seedlings. An increase in mRNA for PP2 during the early stages of seedling growth was also observed by immunoprecipitation of the invitro translation products of hypocotyl polyadenylated RNA. There was close timing in the variations of PP2 synthesised in vivo and in the changes in amounts of translatable PP2-mRNA during the course of seedling growth. A complementary-DNA (cDNA) library to polyadenylated RNA from hypocotyls of 3-d-old Cucurbita seedlings has been constructed. Two cDNA clones, A and B, have been identified by hybrid-release translation to be complementary to the mRNA coding for PP2. The levels of total mRNA for PP2 measured with clone A were found to increase in the first 4 d of seedling growth but decreased to lower levels in older seedlings. Regulatory controls on both transcription and modification of transcripts appeared to occur during the synthesis of PP2. © 1987 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_US
dc.relation.ispartofPlantaen_US
dc.subjectComplementary Dna Cloningen_US
dc.subjectCucurbita (Phloem Differentiation)en_US
dc.subjectLectinen_US
dc.subjectPhloem Differentiationen_US
dc.subjectPhloem Protein (Transcription, Translation)en_US
dc.titleTranscription and translation of phloem protein (PP2) during phloem differentiation in Cucurbita maximaen_US
dc.typeArticleen_US
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_US
dc.identifier.authoritySham, MH=rp00380en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/BF00395032en_US
dc.identifier.scopuseid_2-s2.0-0005339096en_US
dc.identifier.volume170en_US
dc.identifier.issue3en_US
dc.identifier.spage392en_US
dc.identifier.epage399en_US
dc.identifier.isiWOS:A1987G563300014-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridSham, MH=7003729109en_US
dc.identifier.scopusauthoridNorthcote, DH=7006662060en_US

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