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Article: Propofol pretreatment reduces ceramide production and attenuates intestinal mucosal apoptosis induced by intestinal ischemia/reperfusion in rats

TitlePropofol pretreatment reduces ceramide production and attenuates intestinal mucosal apoptosis induced by intestinal ischemia/reperfusion in rats
Authors
Issue Date2008
PublisherLippincott, Williams & Wilkins. The Journal's web site is located at http://www.anesthesia-analgesia.org
Citation
Anesthesia And Analgesia, 2008, v. 107 n. 6, p. 1884-1891 How to Cite?
AbstractBACKGROUND:: Apoptosis has been shown to be a major mode of intestinal epithelial cell death caused by intestinal ischemia/reperfusion (II/R), a condition that is associated with increased oxidative stress. Ceramide has been proposed as a messenger of apoptosis. We investigated if pretreatment with propofol, an anesthetic with antioxidant properties, could reduce ceramide production, and consequently, mucosal epithelial apoptosis induced by II/R in rats. METHODS:: Rat II/R injury was produced by clamping the superior mesenteric artery for 1 h followed by 3 h of reperfusion. Thirty rats were randomly allocated into control, injury (II/R) and propofol (pretreatment) groups (n = 10 per group). In the propofol group, propofol 50 mg/kg, a dose that has been shown to cause the loss of reflex responses to a painful stimulus while remaining sensitive to skin incision in rats, was administered intraperitoneally 30 min before inducing intestinal ischemia, while animals in control and untreated injury groups received an equal volume of intralipid. Intestinal mucosal epithelial apoptosis was detected via electron microscopy and TUNEL analysis. Lipid oxidation product malondialdehyde and the activities of superoxide dismutase were assessed by colorimetric analyses. Ceramide generation and sphingomyelinase mRNA expression in intestinal mucosa were determined by high performance thin layer chromatography and reverse transcriptase polymerase chain reaction, respectively. RESULTS:: II/R caused intestinal mucosal epithelial apoptosis and over-production of ceramide accompanied by up-regulation of sphingomyelinase mRNA expression and increases in lipid oxidation (all P < 0.01 versus control). Propofol pretreatment significantly attenuated these changes (all P < 0.01, propofol versus injury). CONCLUSION:: The findings indicate that propofol pretreatment attenuates II/R-induced intestinal epithelial apoptosis, which might be attributable to its antioxidant property modulating the ceramide pathway. Copyright © 2008 International Anesthesia Research Society.
Persistent Identifierhttp://hdl.handle.net/10722/147263
ISSN
2015 Impact Factor: 3.827
2015 SCImago Journal Rankings: 1.523
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30672021
Funding Information:

Supported, in part, by a grant from National Natural Science Foundation of China (No. 30672021, to KA.L.).

References

 

DC FieldValueLanguage
dc.contributor.authorLiu, KXen_US
dc.contributor.authorChen, SQen_US
dc.contributor.authorHuang, WQen_US
dc.contributor.authorLi, YSen_US
dc.contributor.authorIrwin, MGen_US
dc.contributor.authorXia, Zen_US
dc.date.accessioned2012-05-29T06:01:06Z-
dc.date.available2012-05-29T06:01:06Z-
dc.date.issued2008en_US
dc.identifier.citationAnesthesia And Analgesia, 2008, v. 107 n. 6, p. 1884-1891en_US
dc.identifier.issn0003-2999en_US
dc.identifier.urihttp://hdl.handle.net/10722/147263-
dc.description.abstractBACKGROUND:: Apoptosis has been shown to be a major mode of intestinal epithelial cell death caused by intestinal ischemia/reperfusion (II/R), a condition that is associated with increased oxidative stress. Ceramide has been proposed as a messenger of apoptosis. We investigated if pretreatment with propofol, an anesthetic with antioxidant properties, could reduce ceramide production, and consequently, mucosal epithelial apoptosis induced by II/R in rats. METHODS:: Rat II/R injury was produced by clamping the superior mesenteric artery for 1 h followed by 3 h of reperfusion. Thirty rats were randomly allocated into control, injury (II/R) and propofol (pretreatment) groups (n = 10 per group). In the propofol group, propofol 50 mg/kg, a dose that has been shown to cause the loss of reflex responses to a painful stimulus while remaining sensitive to skin incision in rats, was administered intraperitoneally 30 min before inducing intestinal ischemia, while animals in control and untreated injury groups received an equal volume of intralipid. Intestinal mucosal epithelial apoptosis was detected via electron microscopy and TUNEL analysis. Lipid oxidation product malondialdehyde and the activities of superoxide dismutase were assessed by colorimetric analyses. Ceramide generation and sphingomyelinase mRNA expression in intestinal mucosa were determined by high performance thin layer chromatography and reverse transcriptase polymerase chain reaction, respectively. RESULTS:: II/R caused intestinal mucosal epithelial apoptosis and over-production of ceramide accompanied by up-regulation of sphingomyelinase mRNA expression and increases in lipid oxidation (all P < 0.01 versus control). Propofol pretreatment significantly attenuated these changes (all P < 0.01, propofol versus injury). CONCLUSION:: The findings indicate that propofol pretreatment attenuates II/R-induced intestinal epithelial apoptosis, which might be attributable to its antioxidant property modulating the ceramide pathway. Copyright © 2008 International Anesthesia Research Society.en_US
dc.languageengen_US
dc.publisherLippincott, Williams & Wilkins. The Journal's web site is located at http://www.anesthesia-analgesia.orgen_US
dc.relation.ispartofAnesthesia and Analgesiaen_US
dc.subject.meshAnesthetics, Intravenous - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis - Drug Effectsen_US
dc.subject.meshCeramides - Biosynthesisen_US
dc.subject.meshIntestinal Mucosa - Metabolism - Pathology - Ultrastructureen_US
dc.subject.meshMaleen_US
dc.subject.meshMalondialdehyde - Analysisen_US
dc.subject.meshMicroscopy, Electron, Transmissionen_US
dc.subject.meshPropofol - Pharmacologyen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshReperfusion Injury - Drug Therapy - Metabolism - Pathologyen_US
dc.subject.meshSphingomyelin Phosphodiesterase - Geneticsen_US
dc.subject.meshSuperoxide Dismutase - Metabolismen_US
dc.titlePropofol pretreatment reduces ceramide production and attenuates intestinal mucosal apoptosis induced by intestinal ischemia/reperfusion in ratsen_US
dc.typeArticleen_US
dc.identifier.emailIrwin, MG:mgirwin@hku.hken_US
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_US
dc.identifier.authorityIrwin, MG=rp00390en_US
dc.identifier.authorityXia, Z=rp00532en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1213/ane.0b013e3181884bbfen_US
dc.identifier.pmid19020134en_US
dc.identifier.scopuseid_2-s2.0-58149295458en_US
dc.identifier.hkuros160821-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58149295458&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume107en_US
dc.identifier.issue6en_US
dc.identifier.spage1884en_US
dc.identifier.epage1891en_US
dc.identifier.isiWOS:000261196800021-
dc.publisher.placeUnited Statesen_US

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